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Full-Text Articles in Biomedical Engineering and Bioengineering
Rational Pathway Engineering Of Type I Fatty Acid Synthase Allows The Biosynthesis Of Triacetic Acid Lactone From D-Glucose In Vivo, Wenjuan Zha, Zengyi Shao, John W. Frost, Huimin Zhao
Rational Pathway Engineering Of Type I Fatty Acid Synthase Allows The Biosynthesis Of Triacetic Acid Lactone From D-Glucose In Vivo, Wenjuan Zha, Zengyi Shao, John W. Frost, Huimin Zhao
Zengyi Shao
Metabolic pathway engineering is a powerful tool to synthesize structurally diverse and complex chemicals via genetic manipulation of multistep catalytic systems involved in cell metabolism. Here, we report the rational design of a fatty acid biosynthetic pathway, Brevibacterium ammoniagenes fatty acid synthase B (FAS-B), that allows the microbial synthesis of triacetic acid lactone (TAL) from an inexpensive feedstock, D-glucose. TAL can be chemically converted to phloroglucinol, which is a core structure for the synthesis of various high value bioactive compounds and energetic compounds such as 1,3,5-triamino-2,4,6-trinitrobenzene (TATB). Synthesis of phloroglucinol from d-glucose using this combined biological and chemical synthesis may …
Spatial Analysis Of 3′ Phosphoinositide Signaling In Living Fibroblasts: Ii. Parameter Estimates For Individual Cells From Experiments, Ian C. Schneider, Jason M. Haugh
Spatial Analysis Of 3′ Phosphoinositide Signaling In Living Fibroblasts: Ii. Parameter Estimates For Individual Cells From Experiments, Ian C. Schneider, Jason M. Haugh
Ian C. Schneider
Fibroblast migration is directed by gradients of platelet-derived growth factor (PDGF) during wound healing. As in other chemotactic systems, it has been shown recently that localized stimulation of intracellular phosphoinositide (PI) 3-kinase activity and production of 3′ PI lipids in the plasma membrane are important events in the signaling of spatially biased motility processes. In turn, 3′ PI localization depends on the effective diffusion coefficient, D, and turnover rate constant, k, of these lipids. Here we present a systematic and direct comparison of mathematical model calculations and experimental measurements to estimate the values of the effective 3′ PI diffusion coefficient, …