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Biomedical Engineering and Bioengineering Commons™
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- Keyword
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- Arteriogenesis (2)
- Collateral circulation (2)
- Peripheral arterial occlusive disease (2)
- Automated (1)
- Autostainer (1)
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- Blood Flow (1)
- CO2 control (1)
- Cell imaging (1)
- Cell incubator (1)
- Charged Particles (1)
- Diabetes (1)
- Diagnostics (1)
- Diffuse Layer (1)
- Double Layer (1)
- EPTFE (1)
- Electrophoresis (1)
- Fluorescence (1)
- Fluorescent (1)
- H-Filter (1)
- Histology (1)
- Imaging (1)
- Impaired vasodilation (1)
- Ischemia (1)
- Isoelectric Focusing (1)
- Laser speckle (1)
- Laser speckle contrast imaging (1)
- Live cell imaging (1)
- Mechanoadaptation (1)
- Microfluidic (1)
- Microfluidics (1)
Articles 1 - 10 of 10
Full-Text Articles in Biomedical Engineering and Bioengineering
Live Cell Imaging Gas Control, Elsa M. Bean, Brady C. Berg, Abby A. Jens
Live Cell Imaging Gas Control, Elsa M. Bean, Brady C. Berg, Abby A. Jens
Biomedical Engineering
The following document outlines the entire process for designing and manufacturing a Live Cell Imaging Gas Control subsystem in tandem with the Heated Stage subsystem to make a CO2 and temperature-controlled chamber for use with the Olympus IX73P2F Microscope. It highlights the current literature and patents surrounding live cell imaging and touches on the existing live cell imaging setups currently being used by other entities. Following this, it defines the scope of the project, key customer requirements, and engineering specifications to ensure the design meets all the needs of its users. This document includes project planning steps to establish …
Automated Microscope Stage, Corin Nishimoto, Alison Flesch, Theo Anastos
Automated Microscope Stage, Corin Nishimoto, Alison Flesch, Theo Anastos
Biomedical Engineering
This document seeks to describe the background information, customer requirements, design specifications, indications for use, selected materials, proposed budget, prototypes, final design, manufacturing processes, and testing methods regarding the CellOptimizer automated microscope stage product.
Measuring Blood Flow In The Skeletal Muscle Microcirculation Using Laser Speckle Flowmetry, Kenneth Howard Gouin Iii
Measuring Blood Flow In The Skeletal Muscle Microcirculation Using Laser Speckle Flowmetry, Kenneth Howard Gouin Iii
Biomedical Engineering
The presence of a native collateral circulation, which serves as a natural bypass for blood flow around an occlusion, improves prognosis for patients with ischemic diseases, such as peripheral arterial occlusive disease (PAOD). However, not all patients have a native collateral circulation, and animal models suggest a genetic basis for this variability. In mice, such as the BALB/c, that lack native arteriolar collaterals, neocollateral formation from capillaries that connect two arterial trees can occur after arterial occlusion, resulting in reperfusion of the ischemic watershed. Immature arterialized collateral capillaries (ACCs) at 7 days post arterial occlusion do not vasodilate in response …
Smooth Muscle Cell Organization In The Stem Region Of The Gracilis Collateral Circulation, Amanda Krall
Smooth Muscle Cell Organization In The Stem Region Of The Gracilis Collateral Circulation, Amanda Krall
Biomedical Engineering
Many patients who suffer from the ischemic Peripheral Arterial Occlusive Disease (PAOD) experience intermittent claudication, which can be attributed to impaired vasodilation. Collateral vessels are the primary site of resistance to blood flow downstream; therefore maximizing vasodilation in collaterals is crucial for efficient circulation. Collaterals function as natural bypasses around the occluded arteries and the increase in flow into these vessels causes them to outwardly remodel into conduit vessels. However, functional vasodilation in the stem region of collateral vessels is impaired at day 7 following femoral ligation, which can be attributed to smooth muscle cell malfunction. However, the increase in …
A Microfluidic Pretreatment Isoelectric Focusing Dc-Field Assisted H-Filter For The Separation Of Charged Particles, Cameron Sean Carroll
A Microfluidic Pretreatment Isoelectric Focusing Dc-Field Assisted H-Filter For The Separation Of Charged Particles, Cameron Sean Carroll
Biomedical Engineering
Microfluidic devices are small portable diagnostic chips that allow for analysis of a biologic sample at relatively low cost. This makes them ideal for settings where a hospital is unavailable. The microfluidic pretreatment isoelectric focusing DC-field assisted H-filter is a device that allows for the separation of differently charged particles in a biologic sample. It does this by employing the concepts of diffusion, electrophoresis, and isoelectric focusing. This is perfect for isolation of a desired analyte or separation of waste from a sample in order to achieve better diagnostic results. The device was first modeled mathematically and visually using Comsol. …
Development Of A Blood Flow Measurement Protocol Using Particle Image Velocimetry, Jennifer Chi-Ying Go
Development Of A Blood Flow Measurement Protocol Using Particle Image Velocimetry, Jennifer Chi-Ying Go
Biomedical Engineering
Peripheral Arterial Occlusive Disease (PAOD) is an acquired inflammatory disease where a peripheral artery becomes occluded due to the buildup of atherosclerotic plaques. In patients that possess collateral arteries, an occlusion can lead to shear induced outward remodeling, arteriogenesis, of these collaterals, partially restoring blood flow. However, newly remodeled collaterals exhibit reduced functional vasodilation, which may impair normal activity, such as ambulation. To model chronic ischemia and arteriogenesis in collaterals, a femoral artery ligation in a murine hindlimb is commonly performed. Previous efforts by our group involved measurements of collateral artery diameter to assess the impact of arteriogenesis on functional …
Testing And Validation Of An Autostainer, Nicholas Hanne
Testing And Validation Of An Autostainer, Nicholas Hanne
Biomedical Engineering
Histology performed by Cal Poly students for research projects and labs are currently performed by hand using a dipping technique. This technique is time consuming, expensive, imprecise, and often unrepeatable. Central Coast Pathology donated a Dako Autostainer to the Biomedical Engineering Department that can perform immunohistological stains of up to 48 samples simultaneously using tightly controlled reagent volumes and incubation times. Protocols are programmed into the machine and can be repeated under the same conditions every time with minimal preparation and exposure to potentially dangerous reagents. The autostainer was repaired and validated by comparing PECAM & BBI stains performed on …
Development Of An In-Vitro Hyperglycemic Tissue Engineered Blood Vessel Mimic, Brian C. Wong
Development Of An In-Vitro Hyperglycemic Tissue Engineered Blood Vessel Mimic, Brian C. Wong
Biomedical Engineering
No abstract provided.
Characterization And Analysis Techniques Of A Dynamic In Vitro Blood-Brain Barrier Model, Ryan Everett Woodhouse
Characterization And Analysis Techniques Of A Dynamic In Vitro Blood-Brain Barrier Model, Ryan Everett Woodhouse
Biomedical Engineering
The blood-brain barrier (BBB) is responsible for maintaining the sensitive environment required by the brain. Although the BBB is necessary for proper functioning of the brain, it acts as an obstacle for doctors attempting to treat neurological disease. For a drug to act upon the brain, it must first pass through the discriminating BBB. For this reason, much research has been performed in recent years in order to create an in vitro model of the BBB on which drugs targeted for the central nervous system may be tested. The main goal of this project is to create an in vitro …
Can Spr Be Used To Interrogate The Double Layer Of Nacl, Brandon Kawula
Can Spr Be Used To Interrogate The Double Layer Of Nacl, Brandon Kawula
Biomedical Engineering
Solutions of reagent grade salt .1, .01, .001, .0001, .00001M were run through a SPR to see if SPR had enough resolution to detect the formation of the electric double layer. We found that NaCl did in fact return a detectable signal. The signal shape seems to depend on concentration. While more study may need to be done to get definite information it looks like SPR can interrogate the double layer.