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2019

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Old Dominion University

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Full-Text Articles in Engineering

Ldakm-Eiot: Lightweight Device Authentication And Key Management Mechanism For Edge-Based Iot Deployment, Mohammad Wazid, Ashok Kumar Das, Sachin Shetty, Joel J. P. C. Rodrigues, Youngho Park Dec 2019

Ldakm-Eiot: Lightweight Device Authentication And Key Management Mechanism For Edge-Based Iot Deployment, Mohammad Wazid, Ashok Kumar Das, Sachin Shetty, Joel J. P. C. Rodrigues, Youngho Park

VMASC Publications

In recent years, edge computing has emerged as a new concept in the computing paradigm that empowers several future technologies, such as 5G, vehicle-to-vehicle communications, and the Internet of Things (IoT), by providing cloud computing facilities, as well as services to the end users. However, open communication among the entities in an edge based IoT environment makes it vulnerable to various potential attacks that are executed by an adversary. Device authentication is one of the prominent techniques in security that permits an IoT device to authenticate mutually with a cloud server with the help of an edge node. If authentication …


A Governance Perspective For System-Of-Systems, Polinpapilinho F. Katina, Charles B. Keating, James A. Bobo, Tyrone S. Toland Dec 2019

A Governance Perspective For System-Of-Systems, Polinpapilinho F. Katina, Charles B. Keating, James A. Bobo, Tyrone S. Toland

Engineering Management & Systems Engineering Faculty Publications

The operating landscape of 21st century systems is characteristically ambiguous, emergent, and uncertain. These characteristics affect the capacity and performance of engineered systems/enterprises. In response, there are increasing calls for multidisciplinary approaches capable of confronting increasingly ambiguous, emergent, and uncertain systems. System of Systems Engineering (SoSE) is an example of such an approach. A key aspect of SoSE is the coordination and the integration of systems to enable ‘system-of-systems’ capabilities greater than the sum of the capabilities of the constituent systems. However, there is a lack of qualitative studies exploring how coordination and integration are achieved. The objective of this …


Iomt Malware Detection Approaches: Analysis And Research Challenges, Mohammad Wazid, Ashok Kumar Das, Joel J.P.C. Rodrigues, Sachin Shetty, Youngho Park Dec 2019

Iomt Malware Detection Approaches: Analysis And Research Challenges, Mohammad Wazid, Ashok Kumar Das, Joel J.P.C. Rodrigues, Sachin Shetty, Youngho Park

VMASC Publications

The advancement in Information and Communications Technology (ICT) has changed the entire paradigm of computing. Because of such advancement, we have new types of computing and communication environments, for example, Internet of Things (IoT) that is a collection of smart IoT devices. The Internet of Medical Things (IoMT) is a specific type of IoT communication environment which deals with communication through the smart healthcare (medical) devices. Though IoT communication environment facilitates and supports our day-to-day activities, but at the same time it has also certain drawbacks as it suffers from several security and privacy issues, such as replay, man-in-the-middle, impersonation, …


Electropermeabilization Does Not Correlate With Plasma Membrane Lipid Oxidation, Olga Michel, Andrei G. Pakhomov, Maura Casciola, Jolanta Saczko, Julita Kulbacka, Olga N. Pakhomova Dec 2019

Electropermeabilization Does Not Correlate With Plasma Membrane Lipid Oxidation, Olga Michel, Andrei G. Pakhomov, Maura Casciola, Jolanta Saczko, Julita Kulbacka, Olga N. Pakhomova

Bioelectrics Publications

The permeabilized condition of the cell membrane after electroporation can last minutes but the underlying mechanisms remain elusive. Previous studies suggest that lipid peroxidation could be responsible for the lasting leaky state of the membrane. The present study aims to link oxidation within the plasma membrane of live cells to permeabilization by electric pulses. We have introduced a method for the detection of oxidation by ratiometric fluorescence measurements of BODIPY-C11 dye using total internal reflection fluorescence (TIRF) microscopy, limiting the signal to the cell membrane. CHO-K1 cells were cultured on glass coverslips coated with an electroconductive indium tin oxide (ITO) …


Defining Boat Wake Impacts On Shoreline Stability Toward Management And Policy Solutions, Donna Marie Bilkovic, Molly M. Mitchell, Jennifer Davis, Julie Herman, Elizabeth Andrews, Angela King, Pamela Mason, Navid Tahvildari, Jana Davis, Rachel L. Dixon Dec 2019

Defining Boat Wake Impacts On Shoreline Stability Toward Management And Policy Solutions, Donna Marie Bilkovic, Molly M. Mitchell, Jennifer Davis, Julie Herman, Elizabeth Andrews, Angela King, Pamela Mason, Navid Tahvildari, Jana Davis, Rachel L. Dixon

Civil & Environmental Engineering Faculty Publications

Coastal economies are often supported by activities that rely on commercial or recreational vessels to move people or goods, such as shipping, transportation, cruising, and fishing. Unintentionally, frequent or intense vessel traffic can contribute to erosion of coastlines; this can be particularly evident in sheltered systems where shoreline erosion should be minimal in the absence of boat waves. We reviewed the state of the science of known effects of boat waves on shoreline stability, examined data on erosion, turbidity, and shoreline armoring patterns for evidence of a response to boat waves in Chesapeake Bay, and reviewed existing management and policy …


Estimating Cognitive Workload In An Interactive Virtual Reality Environment Using Eeg, Christoph Tremmel, Christain Herff, Tetsuya Sato, Krzysztof Rechowicz, Yusuke Yamani, Dean J. Krusienski Nov 2019

Estimating Cognitive Workload In An Interactive Virtual Reality Environment Using Eeg, Christoph Tremmel, Christain Herff, Tetsuya Sato, Krzysztof Rechowicz, Yusuke Yamani, Dean J. Krusienski

Electrical & Computer Engineering Faculty Publications

With the recent surge of affordable, high-performance virtual reality (VR) headsets, there is unlimited potential for applications ranging from education, to training, to entertainment, to fitness and beyond. As these interfaces continue to evolve, passive user-state monitoring can play a key role in expanding the immersive VR experience, and tracking activity for user well-being. By recording physiological signals such as the electroencephalogram (EEG) during use of a VR device, the user's interactions in the virtual environment could be adapted in real-time based on the user's cognitive state. Current VR headsets provide a logical, convenient, and unobtrusive framework for mounting EEG …


Electroosmotic Flow Of Viscoelastic Fluid In A Nanochannel Connecting Two Reservoirs, Lanju Mei, Shizhi Qian Nov 2019

Electroosmotic Flow Of Viscoelastic Fluid In A Nanochannel Connecting Two Reservoirs, Lanju Mei, Shizhi Qian

Mechanical & Aerospace Engineering Faculty Publications

Electroosmotic flow (EOF) of viscoelastic fluid with Linear Phan-Thien–Tanner (LPTT) constitutive model in a nanochannel connecting two reservoirs is numerically studied. For the first time, the influence of viscoelasticity on the EOF and the ionic conductance in the micro-nanofluidic interconnect system, with consideration of the electrical double layers (EDLs), is investigated. Regardless of the bulk salt concentration, significant enhancement of the flow rate is observed for viscoelastic fluid compared to the Newtonian fluid, due to the shear thinning effect. An increase in the ionic conductance of the nanochannel occurs for the viscoelastic fluid. The enhancement of the ionic conductance is …


Label-Free Microrna Optical Biosensors, Meimei Lai, Gymama Slaughter Nov 2019

Label-Free Microrna Optical Biosensors, Meimei Lai, Gymama Slaughter

Bioelectrics Publications

MicroRNAs (miRNAs) play crucial roles in regulating gene expression. Many studies show that miRNAs have been linked to almost all kinds of disease. In addition, miRNAs are well preserved in a variety of specimens, thereby making them ideal biomarkers for biosensing applications when compared to traditional protein biomarkers. Conventional biosensors for miRNA require fluorescent labeling, which is complicated, time-consuming, laborious, costly, and exhibits low sensitivity. The detection of miRNA remains a big challenge due to their intrinsic properties such as small sizes, low abundance, and high sequence similarity. A label-free biosensor can simplify the assay and enable the direct detection …


Odu Researchers' Flood Adaptation Study Honored At International Conference, News @ Odu Oct 2019

Odu Researchers' Flood Adaptation Study Honored At International Conference, News @ Odu

News Items

No abstract provided.


Organizational Criteria To Assess Adaptive Capacity In Complex Systems, Abdulrahman Alfaqiri Oct 2019

Organizational Criteria To Assess Adaptive Capacity In Complex Systems, Abdulrahman Alfaqiri

October 11, 2019: Measuring and Evaluating Resilience

No abstract provided.


Paper-Based Flexible Electrode Using Chemically-Modified Graphene And Functionalized Multiwalled Carbon Nanotube Composites For Electrophysiological Signal Sensing, Md Faruk Hossain, Jae Sang Heo, John Nelson, Insoo Kim Oct 2019

Paper-Based Flexible Electrode Using Chemically-Modified Graphene And Functionalized Multiwalled Carbon Nanotube Composites For Electrophysiological Signal Sensing, Md Faruk Hossain, Jae Sang Heo, John Nelson, Insoo Kim

Bioelectrics Publications

Flexible paper-based physiological sensor electrodes were developed using chemically-modified graphene (CG) and carboxylic-functionalized multiwalled carbon nanotube composites (f@MWCNTs). A solvothermal process with additional treatment was conducted to synthesize CG and f@MWCNTs to make CG-f@MWCNT composites. The composite was sonicated in an appropriate solvent to make a uniform suspension, and then it was drop cast on a nylon membrane in a vacuum filter. A number of batches (0%~35% f@MWCNTs) were prepared to investigate the performance of the physical characteristics. The 25% f@MWCNT-loaded composite showed the best adhesion on the paper substrate. The surface topography and chemical bonding of the proposed CG-f@MWCNT …


Dynamic Response Modeling Of High-Speed Planing Craft With Enforced Acceleration, Gene Hou, Brain Johnson, Jonathan Degroff, Steven Trenor, Jennifer Michaeli Oct 2019

Dynamic Response Modeling Of High-Speed Planing Craft With Enforced Acceleration, Gene Hou, Brain Johnson, Jonathan Degroff, Steven Trenor, Jennifer Michaeli

Mechanical & Aerospace Engineering Faculty Publications

An approach is investigated in this study for structural dynamic analysis of a high-speed planing hull, in which the pointwise acceleration data collected from sea trials are enforced as base excitation. The paper first performed the full boat analysis of an 11-meter high speed craft for a period of one wave impact selected from each of nine seakeeping runs. The sea trial acceleration data collected from 11 accelerometers placed close to the centerline and the keel are enforced as input, while those from 3 accelerometers placed around the pilot cabin are selected for validation. The substructure dynamic analysis of the …


A Low-Cost Soft Robotic Hand Exoskeleton For Use In Therapy Of Limited Hand–Motor Function, Grant Rudd, Liam Daly, Vukica Jovanovic, Filip Cukov Sep 2019

A Low-Cost Soft Robotic Hand Exoskeleton For Use In Therapy Of Limited Hand–Motor Function, Grant Rudd, Liam Daly, Vukica Jovanovic, Filip Cukov

Engineering Technology Faculty Publications

We present the design and validation of a low-cost, customizable and 3D-printed anthropomorphic soft robotic hand exoskeleton for rehabilitation of hand injuries using remotely administered physical therapy regimens. The design builds upon previous work done on cable actuated exoskeleton designs by implementing the same kinematic functionality, but with the focus shifted to ease of assembly and cost effectiveness as to allow patients and physicians to manufacture and assemble the hardware necessary to implement treatment. The exoskeleton was constructed solely from 3D-printed and widely available of-the-shelf components. Control of the actuators was realized using an Arduino microcontroller, with a custom-designed shield …


Deposition Of Nb₃Sn Films By Multilayer Sequential Sputtering For Srf Cavity Application, Md. N. Sayeed, Hani E. Elsayed-Ali, U. Pudasaini, G.V. Eremeev, C.E. Reece, M. Burton, A.M. Valente-Feliciano Jul 2019

Deposition Of Nb₃Sn Films By Multilayer Sequential Sputtering For Srf Cavity Application, Md. N. Sayeed, Hani E. Elsayed-Ali, U. Pudasaini, G.V. Eremeev, C.E. Reece, M. Burton, A.M. Valente-Feliciano

Electrical & Computer Engineering Faculty Publications

Nb3Sn is considered as an alternative of Nb for SRF accelerator cavity application due to its potential to obtain higher quality factors and higher accelerating gradients at a higher operating temperature. Magnetron sputtering is one of the effective techniques that can be used to fabricate Nb3Sn on SRF cavity surface. We report on the surface properties of Nb3Sn films fabricated by sputtering multiple layers of Nb and Sn on sapphire and niobium substrates followed by annealing at 950°C for 3 h. The crystal structure, film microstructure, composition and surface roughness were characterized by X-ray …


Learning In Informal Environments Through Engineering Activities Through The Partnership With The Girl Scouts, Vukica M. Jovanovic, Otilia Popescu, Carol Considine, Karina Arcaute, Krishnanand Kaipa, Stephanie G. Adams Jun 2019

Learning In Informal Environments Through Engineering Activities Through The Partnership With The Girl Scouts, Vukica M. Jovanovic, Otilia Popescu, Carol Considine, Karina Arcaute, Krishnanand Kaipa, Stephanie G. Adams

Engineering Technology Faculty Publications

More affordable and portable robots have enabled easier access for outreach activities to happen in different environments. However, exposure to robotics often relies on seeing robots in action, such as industrial robotics and robots that are used for research purposes. Old Dominion University’s College of Engineering and Technology in Norfolk, Virginia recently signed a partnership agreement with the Girl Scouts of Colonial Coast as one of the focused outreach strategies that target the female population. Various events are held on campus in the Hampton Roads residential area located in the southeastern United States, which has a population of around 2 …


Impact Of Bioinspired Robots On Veterans Pursuing Stem Degrees, Otilia Popescu, Vukica M. Jovanovic, Krishnanand Kaipa, Karina Arcaute, Anthony W. Dean Jun 2019

Impact Of Bioinspired Robots On Veterans Pursuing Stem Degrees, Otilia Popescu, Vukica M. Jovanovic, Krishnanand Kaipa, Karina Arcaute, Anthony W. Dean

Engineering Technology Faculty Publications

The gap in the area of advanced manufacturing skilled workforce and the efforts in guiding veterans towards STEM careers are merged in the NSF funded project presented in this paper. While most of the products and STEM educational programs focused on a maker concept that are currently available are specifically designed for young population, at various K-12 grade levels, to increase their interest in STEM and engineering careers in particular, there is a limited availability of such programs to address adult population. The study presented in this paper focuses on developing and implementing a series of workshops for veterans, using …


Teaching Introduction To Welding In Undergraduate And Graduate Engineering Technology Programs, Hamid Eisazadeh, Milan Rakita, Mona Torabizadeh, Vukica Jovanovic Jun 2019

Teaching Introduction To Welding In Undergraduate And Graduate Engineering Technology Programs, Hamid Eisazadeh, Milan Rakita, Mona Torabizadeh, Vukica Jovanovic

Engineering Technology Faculty Publications

Recent trends in the industry have led to an increased need for engineers with welding training. Not many universities in the U.S.A. offer welding courses at undergraduate level. Engineers that do not receive education about this complex process, sometimes they make a very costly and dangerous mistake. More profound understanding of the welding process that expands beyond the basic comprehension of technology use is needed for the advanced manufacturing sector. Welding related courses, as it involves the complex interplay of the four states of matter (solid, liquid, gas and plasma) as governed by the laws of physics, need topics such …


Modulation Of Biological Responses To 2 Ns Electrical Stimuli By Field Reversal, Esin B. Sözer, P. Thomas Vernier Jun 2019

Modulation Of Biological Responses To 2 Ns Electrical Stimuli By Field Reversal, Esin B. Sözer, P. Thomas Vernier

Bioelectrics Publications

Nanosecond bipolar pulse cancellation, a recently discovered Phenomenon, is modulation of the effects of a unipolar electric pulse exposure by a second pulse of opposite polarity. This attenuation of biological response by reversal of the electric field direction has been reported with pulse durations from 60 ns to 900 ns for a wide range of endpoints, and it is not observed with conventional electroporation pulses of much longer duration (> 100 mu s) where pulses are additive regardless of polarity. The most plausible proposed mechanisms involve the field-driven migration of ions to and from the membrane interface (accelerated membrane discharge). …


Effects Of Pulse Width On He Plasma Jets In Contact With Water Evaluated By Oh(A-X) Emission And Ohaq Production, Shutong Song, Esin B. Sözer, Chunqi Jiang Jun 2019

Effects Of Pulse Width On He Plasma Jets In Contact With Water Evaluated By Oh(A-X) Emission And Ohaq Production, Shutong Song, Esin B. Sözer, Chunqi Jiang

Bioelectrics Publications

Nanosecond pulsed helium plasma jets impinging on water produce hydroxyl radicals both in gas- and liquid-phase. In this study, the effects of pulse width on a repetitively pulsed plasma jet in contact with water are evaluated via OH(A-X) emission and OHaq production in water for various pulse widths ranging from 200 to 5000 ns. The maximal energy efficiency of OH(A-X) emission is obtained for pulse widths of 600-800 ns whereas the maximal efficiency of OHaq production is at 200 ns. Temporally-resolved emission spectroscopy shows that more than 40% of OH(A-X) emission is produced during the first 200 ns …


Impact Of Http Cookie Violations In Web Archives, Sawood Alam, Michele C. Weigle, Michael L. Nelson Jun 2019

Impact Of Http Cookie Violations In Web Archives, Sawood Alam, Michele C. Weigle, Michael L. Nelson

Computer Science Faculty Publications

Certain HTTP Cookies on certain sites can be a source of content bias in archival crawls. Accommodating Cookies at crawl time, but not utilizing them at replay time may cause cookie violations, resulting in defaced composite mementos that never existed on the live web. To address these issues, we propose that crawlers store Cookies with short expiration time and archival replay systems account for values in the Vary header along with URIs.


Next-Generation Rainfall Idf Curves For The Virginian Drainage Area Of Chesapeake Bay, Xixi Wang, Xiaomin Yang, Zhaoyi Cai Jun 2019

Next-Generation Rainfall Idf Curves For The Virginian Drainage Area Of Chesapeake Bay, Xixi Wang, Xiaomin Yang, Zhaoyi Cai

Civil & Environmental Engineering Faculty Publications

Probability-based intensity-duration-frequency IDF curves are needed but currently lacking for Department of Defense DoD to construct and manage its infrastructure in changing climate. The objectives of this project were to 1 develop an innovative approach for considering rainfall non-stationarity in developing such IDF curves and 2 apply this approach to the state of Virginia. In this regard, the observed data on 15-min rainfall at 57 gauges and the precipitations projected by twelve pairs of Regional Climate Model RCM and Global Circulation Model GCM were used. For a given gauge or watershed, in terms of fitting the empirical exceedance probabilities, a …


5th/6th Graders And Preservice Teachers Explore Engineering And Coding In A Combined After-School Technology Club/Educational Technology Course, Jennifer Kidd, Sam Sacks, Krishnanand Kaipa, Kelly Rippard Mar 2019

5th/6th Graders And Preservice Teachers Explore Engineering And Coding In A Combined After-School Technology Club/Educational Technology Course, Jennifer Kidd, Sam Sacks, Krishnanand Kaipa, Kelly Rippard

Teaching & Learning Faculty Publications

Elementary schools are increasingly encouraged to adopt STEM education efforts that include engineering and programming, yet pre-service teachers (PSTs) are not traditionally prepared to teach these subjects. This pilot study describes an innovative preparation experience designed to help PSTs gain experience and confidence in these areas. As part of an undergraduate educational technology course, PSTs led an after-school technology club at a local public school. PSTs enrolled in the course worked alongside fifth and sixth grade students on a series of collaborative design challenges that culminated in the creation of animal-inspired robots. The “WoW Club”, as it came to be …


Framework For Improving Complex System Performance, Charles B. Keating, Polinpapilinho F. Katina, Ra'ed Jaradat, Joseph M. Bradley, Richard Hodge Jan 2019

Framework For Improving Complex System Performance, Charles B. Keating, Polinpapilinho F. Katina, Ra'ed Jaradat, Joseph M. Bradley, Richard Hodge

Engineering Management & Systems Engineering Faculty Publications

This paper introduces a framework for improvement of complex system performance. Complex systems are besieged with conditions marked by increasing uncertainty, emergence, and ambiguity. Additionally, demands for increased productivity, resource efficiencies, and performance improvement make new approaches paramount for modern systems engineers. In response, a framework to improve complex system performance is developed. Following an introduction, the paper pursues four objectives: (1) introduction of Complex System Governance (CSG) as a foundation to describe essential system functions, (2) suggest system `pathologies' as an explanation for deep system performance issues, (3) exploration of system performance improvement as a function of `requisite variety' …


A Fully-Flexible Solution-Processed Autonomous Glucose Indicator, Jonathan D. Yuen, Ankit Baingane, Qumrul Hasan, Lisa C. Shriver-Lake, Scott A. Walper, Daniel Zabetakis, Joyce C. Breger, David A. Stenger, Gymama Slaughter Jan 2019

A Fully-Flexible Solution-Processed Autonomous Glucose Indicator, Jonathan D. Yuen, Ankit Baingane, Qumrul Hasan, Lisa C. Shriver-Lake, Scott A. Walper, Daniel Zabetakis, Joyce C. Breger, David A. Stenger, Gymama Slaughter

Bioelectrics Publications

We present the first demonstration of a fully-flexible, self-powered glucose indicator system that synergizes two flexible electronic technologies: a flexible self-powering unit in the form of a biofuel cell, with a flexible electronic device - a circuit-board decal fabricated with biocompatible microbial nanocellulose. Our proof-of-concept device, comprising an enzymatic glucose fuel cell, glucose sensor and a LED indicator, does not require additional electronic equipment for detection or verification; and the entire structure collapses into a microns-thin, self-adhering, single-centimeter-square decal, weighing less than 40 mg. The flexible glucose indicator system continuously operates a light emitting diode (LED) through a capacitive charge/discharge …


Effect Of Cold Atmospheric Plasma Treatment On The Metabolites Of Human Leukemia Cells, Dehui Xu, Ning Ning, Yujing Xu, Bingchuan Wang, Qingjie Cui, Zhijie Liu, Xiaohua Wang, Dingxin Liu, Hailan Chen, Michael G. Kong Jan 2019

Effect Of Cold Atmospheric Plasma Treatment On The Metabolites Of Human Leukemia Cells, Dehui Xu, Ning Ning, Yujing Xu, Bingchuan Wang, Qingjie Cui, Zhijie Liu, Xiaohua Wang, Dingxin Liu, Hailan Chen, Michael G. Kong

Bioelectrics Publications

Background

Acute myeloid leukemia (AML) is a typically fatal malignancy and new drug and treatment need to be developed for a better survival outcome. Cold atmospheric plasma (CAP) is a novel technology, which has been widely applied in biomedicine, especially in various of cancer treatment. However, the changes in cell metabolism after CAP treatment of leukemia cells have been rarely studied.

Methods

In this study, we investigated the metabolite profiling of plasma treatment on leukemia cells based on Gas Chromatography Tandem Time-of-Flight Mass Spectrometry (GC-TOFMS). Simultaneously, we conducted a series of bioinformatics analysis of metabolites and metabolic pathways with significant …


Nanosecond Pulsed Electric Signals Can Affect Electrostatic Environment Of Protiens Below The Threshold Of Conformational Effects: The Case Study Of Sod1 With A Molecular Simulation Study, Elena Della Valle, Paolo Marracino, Olga Pakhomova, Micaela Liberti, Francesca Apollonio Jan 2019

Nanosecond Pulsed Electric Signals Can Affect Electrostatic Environment Of Protiens Below The Threshold Of Conformational Effects: The Case Study Of Sod1 With A Molecular Simulation Study, Elena Della Valle, Paolo Marracino, Olga Pakhomova, Micaela Liberti, Francesca Apollonio

Bioelectrics Publications

Electric fields can be a powerful tool to interact with enzymes or proteins, with an intriguing perspective to allow protein manipulation. Recently, researchers have focused the interest on intracellular enzyme modifications triggered by the application of nanosecond pulsed electric fields. These findings were also supported by theoretical predictions from molecular dynamics simulations focussing on significant variations in protein secondary structures. In this work, a theoretical study utilizing molecular dynamics simulations is proposed to explore effects of electric fields of high intensity and very short nanosecond duration applied to the superoxide dismutase (Cu/Zn-SOD or SOD-1), an important enzyme involved in the …


Mechanisms And Immunogenicity Of Nspef-Induced Cell Death In B16f10 Melanoma Tumors, Alessandra Rossi, Olga N. Pakhomova, Andrei G. Pakhomov, Samantha Weygandt, Anna A. Bulysheva, Len E. Murray, Peter A. Mollica, Claudia Muratori Jan 2019

Mechanisms And Immunogenicity Of Nspef-Induced Cell Death In B16f10 Melanoma Tumors, Alessandra Rossi, Olga N. Pakhomova, Andrei G. Pakhomov, Samantha Weygandt, Anna A. Bulysheva, Len E. Murray, Peter A. Mollica, Claudia Muratori

Bioelectrics Publications

Accumulating data indicates that some cancer treatments can restore anticancer immunosurveillance through the induction of tumor immunogenic cell death (ICD). Nanosecond pulsed electric fields (nsPEF) have been shown to efficiently ablate melanoma tumors. In this study we investigated the mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors. Our data show that in vitro nsPEF (20-200, 200-ns pulses, 7 kV/cm, 2 Hz) caused a rapid dose-dependent cell death which was not accompanied by caspase activation or PARP cleavage. The lack of nsPEF-induced apoptosis was confirmed in vivo in B16F10 tumors. NsPEF also failed to trigger ICD-linked responses such …


Nanosecond Pulsed Electric Fields Induce Endoplasmic Reticulum Stress Accompanied By Immunogenic Cell Death In Murine Models Of Lymphoma And Colorectal Cancer, Alessandra Rossi, Olga N. Pakhomova, Peter A. Mollica, Maura Casciola, Uma Mangalanathan, Andrei G. Pakhomov, Claudia Muratori Jan 2019

Nanosecond Pulsed Electric Fields Induce Endoplasmic Reticulum Stress Accompanied By Immunogenic Cell Death In Murine Models Of Lymphoma And Colorectal Cancer, Alessandra Rossi, Olga N. Pakhomova, Peter A. Mollica, Maura Casciola, Uma Mangalanathan, Andrei G. Pakhomov, Claudia Muratori

Bioelectrics Publications

Depending on the initiating stimulus, cancer cell death can be immunogenic or non-immunogenic. Inducers of immunogenic cell death (ICD) rely on endoplasmic reticulum (ER) stress for the trafficking of danger signals such as calreticulin (CRT) and ATP. We found that nanosecond pulsed electric fields (nsPEF), an emerging new modality for tumor ablation, cause the activation of the ER-resident stress sensor PERK in both CT-26 colon carcinoma and EL-4 lymphoma cells. PERK activation correlates with sustained CRT exposure on the cell plasma membrane and apoptosis induction in both nsPEF-treated cell lines. Our results show that, in CT-26 cells, the activity of …


Charge Storage In Wo³ Polymorphs And Their Application As Supercapacitor Electrode Material, Vaibhav Lokhande, Abhishek Lokhande, Gon Namkoong, Jin Hyeok Kim, Taeksoo Ji Jan 2019

Charge Storage In Wo³ Polymorphs And Their Application As Supercapacitor Electrode Material, Vaibhav Lokhande, Abhishek Lokhande, Gon Namkoong, Jin Hyeok Kim, Taeksoo Ji

Electrical & Computer Engineering Faculty Publications

Tungsten oxide is a versatile material with different applications. It has many polymorphs with varying performance in energy storage application. We report simple and facile way to synthesize four phases of tungsten oxide from same precursor materials only by changing the pH and temperature values. Monoclinic, hexagonal, orthorhombic and tetragonal phase obtained, were analyzed and tested for supercapacitor application. The electrochemical analysis of four phases indicates that the hexagonal phase is best-suited electrode material for supercapacitor. The hexagonal phase exhibits higher specific capacitance (377.5 Fg-1 at 2 mVs-1), higher surface capacitive contribution (75%), better stability and rate …


Microstructural Evaluation Of Aluminium Alloy A365 T6 In Machining Operation, Bankole I. Oladapo, S. Abolfazl Zahedi, Francis T. Omigbodun, Edwin A. Oshin, Victor A. Adebiyi, Olaoluwa B. Malachi Jan 2019

Microstructural Evaluation Of Aluminium Alloy A365 T6 In Machining Operation, Bankole I. Oladapo, S. Abolfazl Zahedi, Francis T. Omigbodun, Edwin A. Oshin, Victor A. Adebiyi, Olaoluwa B. Malachi

Electrical & Computer Engineering Faculty Publications

The optimum cutting parameters such as cutting depth, feed rate, cutting speed and magnitude of the cutting force for A356 T6 was determined concerning the microstructural detail of the material. Novel test analyses were carried out, which include mechanical evaluation of the materials for density, glass transition temperature, tensile and compression stress, frequency analysis and optimisation as well as the functional analytic behaviour of the samples. The further analytical structure of the particle was performed, evaluating the surface luminance structure and the profile structure. The cross-sectional filter profile of the sample was extracted, and analyses of Firestone curve for the …