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Full-Text Articles in Engineering

Vitamin A Deficiency Impairs The Immune Response To Intranasal Vaccination And Rsv Infection In Neonatal Calves, Jodi L. Mcgill, Sean M. Kelly, Mariana Guerra-Maupome, Emma Winkley, Jamie Henningson, Balaji Narasimhan, Randy E. Sacco Oct 2019

Vitamin A Deficiency Impairs The Immune Response To Intranasal Vaccination And Rsv Infection In Neonatal Calves, Jodi L. Mcgill, Sean M. Kelly, Mariana Guerra-Maupome, Emma Winkley, Jamie Henningson, Balaji Narasimhan, Randy E. Sacco

Chemical and Biological Engineering Publications

Respiratory syncytial virus (RSV) infection is a leading cause of severe acute lower respiratory tract infection in infants and children worldwide. Vitamin A deficiency (VAD) is one of the most prevalent nutrition-related health problems in the world and is a significant risk factor in the development of severe respiratory infections in infants and young children. Bovine RSV (BRSV) is a primary cause of lower respiratory tract disease in young cattle. The calf model of BRSV infection is useful to understand the immune response to human RSV infection. We have previously developed an amphiphilic polyanhydride nanoparticle (NP)-based vaccine (i.e ...


Quantifying Vehicle Control From Physiology In Type 1 Diabetes, Pranamesh Chakraborty, Jennifer Merickel, Viraj Shah, Anuj Sharma, Chinmay Hegde, Cyrus Desouza, Andjela Drincic, Pujitha Gunaratne, Matthew Rizzo Oct 2019

Quantifying Vehicle Control From Physiology In Type 1 Diabetes, Pranamesh Chakraborty, Jennifer Merickel, Viraj Shah, Anuj Sharma, Chinmay Hegde, Cyrus Desouza, Andjela Drincic, Pujitha Gunaratne, Matthew Rizzo

Civil, Construction and Environmental Engineering Publications

Objective: Our goal is to measure real-world effects of at-risk driver physiology on safety-critical tasks like driving by monitoring driver behavior and physiology in real-time. Drivers with type 1 diabetes (T1D) have an elevated crash risk that is linked to abnormal blood glucose, particularly hypoglycemia. We tested the hypotheses that (1) T1D drivers would have overall impaired vehicle control behavior relative to control drivers without diabetes, (2) At-risk patterns of vehicle control in T1D drivers would be linked to at-risk, in-vehicle physiology, and (3) T1D drivers would show impaired vehicle control with more recent hypoglycemia prior to driving.

Methods: Drivers ...


At The Intersection Of Industry, Academia, And Government: How Do We Facilitate Productive Precision Livestock Farming In Practice?, Brett C. Ramirez, Hongwei Xin, Patrick G. Halbur, Donald H. Beermann, Stephanie L. Hansen, Daniel C. L. Linhares, Joshua M. Peschel, Christopher J. Rademacher, James M. Reecy, Jason W. Ross, Timothy A. Shepherd, James E. Koltes Aug 2019

At The Intersection Of Industry, Academia, And Government: How Do We Facilitate Productive Precision Livestock Farming In Practice?, Brett C. Ramirez, Hongwei Xin, Patrick G. Halbur, Donald H. Beermann, Stephanie L. Hansen, Daniel C. L. Linhares, Joshua M. Peschel, Christopher J. Rademacher, James M. Reecy, Jason W. Ross, Timothy A. Shepherd, James E. Koltes

Veterinary Diagnostic and Production Animal Medicine Publications

This commentary is a comprehensive synthesis of ideas generated from a workshop, hosted by Iowa State University, encompassing precision livestock farming (PLF) research and applications for industry–academia. The goal of this workshop was to demonstrate existing institution research and strategically propel further PLF development and industry adoption. Six key thematic areas were identified from participant discussion: sensors and algorithms, implementation, economic feasibility, data, rural and societal impacts, and education and training. These themes were used to focus discussion on identifying the new knowledge needed to drive implementation and examine current and future challenges of implementing PLF. At the convergence ...


Zinc-Chelating Mechanism Of Sea Cucumber (Stichopus Japonicus)-Derived Synthetic Peptides, Xiaoyang Liu, Zixu Wang, Fawen Yin, Yuxin Liu, Ningbo Qin, Yoshimasa Nakamura, Fereidoon Shahidi, Chenxu Yu, Dayong Zhou, Beiwei Zhu Jul 2019

Zinc-Chelating Mechanism Of Sea Cucumber (Stichopus Japonicus)-Derived Synthetic Peptides, Xiaoyang Liu, Zixu Wang, Fawen Yin, Yuxin Liu, Ningbo Qin, Yoshimasa Nakamura, Fereidoon Shahidi, Chenxu Yu, Dayong Zhou, Beiwei Zhu

Agricultural and Biosystems Engineering Publications

In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively ...


Phlorotannins From Undaria Pinnatifida Sporophyll: Extraction, Antioxidant, And Anti-Inflammatory Activities, Xiufang Dong, Ying Bai, Zhe Xu, Yixin Shi, Yihan Sun, Srinivas Janaswamy, Chenxu Yu, Hang Qi Jul 2019

Phlorotannins From Undaria Pinnatifida Sporophyll: Extraction, Antioxidant, And Anti-Inflammatory Activities, Xiufang Dong, Ying Bai, Zhe Xu, Yixin Shi, Yihan Sun, Srinivas Janaswamy, Chenxu Yu, Hang Qi

Agricultural and Biosystems Engineering Publications

Undaria pinnatifida sporophyll (U. pinnatifida) is a major byproduct of U. pinnatifida (a brown algae) processing. Its phenolic constituents, phlorotannins, are of special interest due to their intrinsic ability to precipitate proteins. Herein, a high-temperature extraction procedure was used to isolate these biologically active compounds. The heating temperature, heating time, and extraction solvent (ethanol) concentration were examined with response surface analysis to determine the optimal conditions to achieve the maximum extraction rate. The analysis revealed the optimal conditions to be: 170 °C of heating temperature, 5.2 h of heating time, and 52% ethanol concentration for a yield of 10 ...


Pentaerythritol-Based Lipid A Bolsters The Antitumor Efficacy Of A Polyanhydride Particle-Based Cancer Vaccine, Emad I. Wafa, Sean M. Geary, Kathleen A. Ross, Jonathan T. Goodman, Balaji Narasimhan, Aliasger K. Salem Jul 2019

Pentaerythritol-Based Lipid A Bolsters The Antitumor Efficacy Of A Polyanhydride Particle-Based Cancer Vaccine, Emad I. Wafa, Sean M. Geary, Kathleen A. Ross, Jonathan T. Goodman, Balaji Narasimhan, Aliasger K. Salem

Chemical and Biological Engineering Publications

The primary objective of this study was to enhance the antitumor efficacy of a model cancer vaccine through co-delivery of pentaerythritol lipid A (PELA), an immunological adjuvant, and a model tumor antigen, ovalbumin (OVA), separately loaded into polyanhydride particles (PA). In vitro experiments showed that encapsulation of PELA into PA (PA-PELA) significantly enhanced its stimulatory capacity on dendritic cells as evidenced by increased levels of the cell surface costimulatory molecules, CD80/CD86. In vivo experiments showed that PA-PELA, in combination with OVA-loaded PA (PA-OVA), significantly expanded the OVA-specific CD8+ T lymphocyte population compared to PA-OVA alone. Furthermore, serum OVA-specific antibody ...


Quantification Of Site Layout And Filter Characteristics On Primary Filter Airflow Reduction On Commercial Swine Sites In Iowa, Benjamin Smith, Steven Hoff, Jay Harmon, Daniel Andersen, Jeffrey Zimmerman, John Stinn Jun 2019

Quantification Of Site Layout And Filter Characteristics On Primary Filter Airflow Reduction On Commercial Swine Sites In Iowa, Benjamin Smith, Steven Hoff, Jay Harmon, Daniel Andersen, Jeffrey Zimmerman, John Stinn

Agricultural and Biosystems Engineering Publications

Fresh air intake filtration is used on commercial swine breeding-gestation-farrowing farms to reduce the frequency of airborne infectious agents. For swine producers, porcine reproductive and respiratory syndrome virus (PRRSV), influenza A virus and Mycoplasma hyopneumoniae are considered the most economically challenging airborne pathogens. Reduced frequency of disease outbreaks has been attributed to retrofitting existing systems with filtration. Economic analysis of operating costs includes energy use, maintenance and replacement of filters. Filter replacement, the largest operational cost, is dependent on filter lifespan. However, limited data is available on filter lifespan and the rate of airflow reduction during the high dust-loading periods ...


Computational Cardiovascular Flow Analysis With The Variational Multiscale Methods, Kenji Takizawa, Yuri Bazilevs, Tayfun E. Tezduyar, Ming-Chen Hsu Jun 2019

Computational Cardiovascular Flow Analysis With The Variational Multiscale Methods, Kenji Takizawa, Yuri Bazilevs, Tayfun E. Tezduyar, Ming-Chen Hsu

Mechanical Engineering Publications

Computational cardiovascular flow analysis can provide valuable information to medical doctors in a wide range of patientspecific cases, including cerebral aneurysms, aortas and heart valves. The computational challenges faced in this class of flow analyses also have a wide range. They include unsteady flows, complex cardiovascular geometries, moving boundaries and interfaces, such as the motion of the heart valve leaflets, contact between moving solid surfaces, such as the contact between the leaflets, and the fluid–structure interaction between the blood and the cardiovascular structure. Many of these challenges have been or are being addressed by the Space–Time Variational Multiscale ...


Vitamin C And B3 As New Biomaterials To Alter Intestinal Stem Cells, Yijun Qi, Jo Lohman, Kaitlin M. Bratlie, Nathan Peroutka-Bigus, Bryan Bellaire, Michael Wannemeuhler, Kyoung-Jin Yoon, Terrence A. Barrett, Qun Wang May 2019

Vitamin C And B3 As New Biomaterials To Alter Intestinal Stem Cells, Yijun Qi, Jo Lohman, Kaitlin M. Bratlie, Nathan Peroutka-Bigus, Bryan Bellaire, Michael Wannemeuhler, Kyoung-Jin Yoon, Terrence A. Barrett, Qun Wang

Materials Science and Engineering Publications

Vitamin C (ascorbic acid) and vitamin B3 (niacin) have been extensively studied since the 20th century. In the area of stem cell biology, vitamin C has shown its direct impact towards homeostasis and epigenetic changes.1 Vitamin B3 aids in maintaining healthy intestinal homeostasis and reducing gut inflammation by participating in the rapamycin signaling pathway.2 In this study, vitamin C and vitamin B3 (600 and 1200 μg/ml) have been explored as potential new biomaterials to study their effects on four types of intestinal stem cells which are isolated from mice bearing different microbiota. We observed that C3H ASF ...


Investigation Of Characteristic Rippling Topology Produced During Uhmwpe Sliding, Kevin G. Plumlee, Christian J. Schwartz Apr 2019

Investigation Of Characteristic Rippling Topology Produced During Uhmwpe Sliding, Kevin G. Plumlee, Christian J. Schwartz

Mechanical Engineering Publications

Ultra-high molecular weight polyethylene (UHMWPE) remains the sole polymer bearing material for use in orthopedic implants and its behavior has been studied extensively. Various techniques, such as crosslinking, have been employed to reduce the total wear volume. However, little is known about the actual wear mechanisms that contribute to the removal of debris from the bulk. A nearly universal observation of the wear surfaces after multidirectional sliding of UHMWPE is the presence of regularly-spaced parallel ripples whose wavelength closely matches debris size. In spite of the fact that this phenomenon has been observed for decades in both retrieved implants as ...


Effects Of Litter Floor Access And Inclusion Of Experienced Hens In Aviary Housing On Floor Eggs, Litter Condition, Air Quality, And Hen Welfare, Jofran L. Oliveira, Hongwei Xin, Lilong Chai, Suzanne T. Millman Apr 2019

Effects Of Litter Floor Access And Inclusion Of Experienced Hens In Aviary Housing On Floor Eggs, Litter Condition, Air Quality, And Hen Welfare, Jofran L. Oliveira, Hongwei Xin, Lilong Chai, Suzanne T. Millman

Agricultural and Biosystems Engineering Publications

To better understand how relevant intensive systems’ characteristics simultaneously affect the performance and welfare of broiler chickens, a meta-analysis of recent literature was carried out. The study determined the effects of gender, genetics, experimental initial age (EIA, d), stocking density (SD; kg/m2), group size (GS; n), bedding material (yes/no), duration of photoperiod (DP; h), divided scotoperiod (yes/no), feeding phases (1/2/3/>3), environmental control (EC; yes/no), environmental enrichment (yes/no), use of vaccines and other medications (yes/no), experimental duration (d), and relevant 2-way interactions on average daily gain (g/d), average daily feed intake ...


Amphiphilic Polyanhydride-Based Recombinant Muc4Β-Nanovaccine Activates Dendritic Cells, Kasturi Banerjee, Shailendra K. Gautam, Prakash Kshirsagar, Kathleen A. Ross, Gaelle Spagnol, Paul Sorgen, Michael J. Wannemeuhler, Balaji Narasimhan, Joyce C. Solheim, Sushil Kumar, Surinder K. Batra, Maneesh Jain Mar 2019

Amphiphilic Polyanhydride-Based Recombinant Muc4Β-Nanovaccine Activates Dendritic Cells, Kasturi Banerjee, Shailendra K. Gautam, Prakash Kshirsagar, Kathleen A. Ross, Gaelle Spagnol, Paul Sorgen, Michael J. Wannemeuhler, Balaji Narasimhan, Joyce C. Solheim, Sushil Kumar, Surinder K. Batra, Maneesh Jain

Chemical and Biological Engineering Publications

Mucin 4 (MUC4) is a high molecular weight glycoprotein that is differentially overexpressed in pancreatic cancer (PC), functionally contributes to disease progression, and correlates with poor survival. Further, due to its aberrant glycosylation and extensive splicing, MUC4 is a potential target for cancer immunotherapy. Our previous studies have demonstrated the utility of amphiphilic polyanhydride nanoparticles as a useful platform for the development of protein-based prophylactic and therapeutic vaccines. In the present study, we encapsulated purified recombinant human MUC4-beta (MUC4β) protein in polyanhydride (20:80 CPTEG:CPH) nanoparticles (MUC4β-nanovaccine) and evaluated its ability to activate dendritic cells and induce adaptive immunity ...


Pentablock Copolymer Micelle Nanoadjuvants Enhance Cytosolic Delivery Of Antigen And Improve Vaccine Efficacy While Inducing Low Inflammation, Sujata Senapati, Ross J. Darling, Darren Loh, Ian C. Schneider, Michael J. Wannemeuhler, Balaji Narasimhan, Surya K. Mallapragada Feb 2019

Pentablock Copolymer Micelle Nanoadjuvants Enhance Cytosolic Delivery Of Antigen And Improve Vaccine Efficacy While Inducing Low Inflammation, Sujata Senapati, Ross J. Darling, Darren Loh, Ian C. Schneider, Michael J. Wannemeuhler, Balaji Narasimhan, Surya K. Mallapragada

Chemistry Publications

As the focus has shifted from traditional killed or live, attenuated vaccines towards subunit vaccines, improvements in vaccine safety have been confronted with low immunogenicity of protein antigens. This issue has been addressed by synthesizing and designing a wide variety of antigen carriers and adjuvants, such as Toll-like receptor agonists (e.g., MPLA, CpG). Studies have focused on optimizing adjuvants for improved cellular trafficking, cytosolic availability, and improved antigen presentation. In this work, we describe the design of novel amphiphilic pentablock copolymer (PBC) adjuvants that exhibit high biocompatibility and reversible pH- and temperature-sensitive micelle formation. We demonstrate improved humoral immunity ...


Data Evidencing Slow Anaerobic Digestion In Emergency Treatment And Disposal Of Infectious Animal Carcasses, Jacek A. Koziel, Heekwon Ahn, Thomas D. Glanville, Timothy S. Frana, J. (Hans) Van Leeuwen, Lam T. Nguyen Feb 2019

Data Evidencing Slow Anaerobic Digestion In Emergency Treatment And Disposal Of Infectious Animal Carcasses, Jacek A. Koziel, Heekwon Ahn, Thomas D. Glanville, Timothy S. Frana, J. (Hans) Van Leeuwen, Lam T. Nguyen

Agricultural and Biosystems Engineering Publications

Burial of infectious and potentially infectious livestock and poultry animals is the most common response to an emergency situation. The data set summarizes 22-week-long experiment that simulates the environment found within conventional burial trenches for emergency disposal of animal carcasses, worldwide, sometimes with a topical application of quicklime as it is required in the Republic of Korea. This data set shows the rarely presented evidence of the extremely slow decay of animal carcasses. Besides visual evidence of no visible breakdown of carcass material, i.e., carcass (or carcass quarters and coarse cuts) still resembled the initial material at the end ...


Use Of Flexible Sensor To Characterize Biomechanics Of Canine Skin, Austin R. J. Downey, Jin Yan, Eric M. Zellner, Karl H. Kraus, Iris V. Rivero, Simon Laflamme Jan 2019

Use Of Flexible Sensor To Characterize Biomechanics Of Canine Skin, Austin R. J. Downey, Jin Yan, Eric M. Zellner, Karl H. Kraus, Iris V. Rivero, Simon Laflamme

Civil, Construction and Environmental Engineering Publications

Background: Suture materials and techniques are frequently evaluated in ex vivo studies by comparing tensile strengths. However, the direct measurement techniques to obtain the tensile forces in canine skin are not available, and, therefore, the conditions suture lines undergo is unknown. A soft elastomeric capacitor is used to monitor deformation in the skin over time by sensing strain. This sensor was applied to a sample of canine skin to evaluate its capacity to sense strain in the sample while loaded in a dynamic material testing machine. The measured strain of the sensor was compared with the strain measured by the ...


Vaccine Distribution Strategies Against Polio: An Analysis Of Turkey Scenario, Elif Elçin Günay, Kijung Park, Sena Aydoğan, Gül E. Okudan Kremer Jan 2019

Vaccine Distribution Strategies Against Polio: An Analysis Of Turkey Scenario, Elif Elçin Günay, Kijung Park, Sena Aydoğan, Gül E. Okudan Kremer

Industrial and Manufacturing Systems Engineering Conference Proceedings and Posters

As a result of the ongoing Syrian civil war, almost 3 million refugees moved to Turkey since 2011 because of security reasons. However, the government operated refugee camps have been largely inadequate to accommodate this huge number of refugees. Therefore, almost 91% of the Syrian refugees live out of government-operated camps. According to a Turkish Disaster and Emergency Management Agency (AFAD) report, 45.4% of the children under 5 years old who live out of camps are not vaccinated against polio. This presents a serious health threat to the local population and the whole region. In order to deal with ...


Design And Synthesis Of Multivalent Α-1,2-Trimannose-Linked Bioerodible Microparticles For Applications In Immune Response Studies Of Leishmania Major Infection, Chelsea L. Rintelmann, Tara Grinnage-Pulley, Kathleen Ross, Daniel E. K. Kabotso, Angela Toepp, Anne Cowell, Christine Petersen, Balaji Narasimhan, Nicola Pohl Jan 2019

Design And Synthesis Of Multivalent Α-1,2-Trimannose-Linked Bioerodible Microparticles For Applications In Immune Response Studies Of Leishmania Major Infection, Chelsea L. Rintelmann, Tara Grinnage-Pulley, Kathleen Ross, Daniel E. K. Kabotso, Angela Toepp, Anne Cowell, Christine Petersen, Balaji Narasimhan, Nicola Pohl

Chemical and Biological Engineering Publications

Leishmaniasis, a neglected tropical disease, currently infects approximately 12 million people worldwide with 1 to 2 million new cases each year in predominantly underdeveloped countries. The treatment of the disease is severely underdeveloped due to the ability of the Leishmania pathogen to evade and abate immune responses. In an effort to develop anti-leishmaniasis vaccines and adjuvants, novel carbohydrate-based probes were made to study the mechanisms of immune modulation. In this study, a new bioerodible polyanhydride microparticle was designed and conjugated with a glycodendrimer molecular probe. This molecular probe incorporates a pathogen-like multivalent display of α-1,2-trimannose, for which a more ...


Factors Affecting Pre-Filter Loading And Strategies To Manage Excessive Loading, Benjamin C. Smith, Steven J. Hoff, Jay D. Harmon, Daniel S. Andersen, Jeffrey J. Zimmerman, John P. Stinn Jan 2019

Factors Affecting Pre-Filter Loading And Strategies To Manage Excessive Loading, Benjamin C. Smith, Steven J. Hoff, Jay D. Harmon, Daniel S. Andersen, Jeffrey J. Zimmerman, John P. Stinn

Agricultural and Biosystems Engineering Conference Proceedings and Presentations

Ventilation air filtration is becoming a popular method to control airborne transmission of diseases on commercial sow farms. For example, air filtration can reduce the frequency of airborne outbreaks of porcine reproductive and respiratory syndrome virus (PRRSV). The capital investment is justified on the basis of diseases losses avoided, but better data on filter lifespan is needed to give producers a more complete understanding of all costs related to air filtration. In Objective 1 a 6-month (May-to-November) intensive study was done on commercial sow farms to determine factors affecting pre-filter lifespan. In Objective 2, a study into methods to prolong ...


Interleukin-1 Alpha Increases Anti-Tumor Efficacy Of Cetuximab In Head And Neck Squamous Cell Carcinoma, Madelyn Espinosa-Cotton, Samuel N. Rodman Iii, Kathleen A. Ross, Isaac J. Jensen, Kenley Sangodeyi-Miller, Ayana J. Mclaren, Rachel A. Dahl, Katherine N. Gibson-Corley, Adam T. Koch, Yang-Xin Fu, Vladimir P. Badovinac, Douglas Laux, Balaji Narasimhan, Andrean L. Simons Jan 2019

Interleukin-1 Alpha Increases Anti-Tumor Efficacy Of Cetuximab In Head And Neck Squamous Cell Carcinoma, Madelyn Espinosa-Cotton, Samuel N. Rodman Iii, Kathleen A. Ross, Isaac J. Jensen, Kenley Sangodeyi-Miller, Ayana J. Mclaren, Rachel A. Dahl, Katherine N. Gibson-Corley, Adam T. Koch, Yang-Xin Fu, Vladimir P. Badovinac, Douglas Laux, Balaji Narasimhan, Andrean L. Simons

Chemical and Biological Engineering Publications

Background: Despite the high prevalence of epidermal growth factor receptor (EGFR) overexpression in head and neck squamous cell carcinomas (HNSCCs), incorporation of the EGFR inhibitor cetuximab into the clinical management of HNSCC has not led to significant changes in long-term survival outcomes. Therefore, the identification of novel therapeutic approaches to enhance the clinical efficacy of cetuximab could lead to improved long-term survival for HNSCC patients. Our previous work suggests that EGFR inhibition activates the interleukin-1 (IL-1) pathway via tumor release of IL-1 alpha (IL-1α), although the clinical implications of activating this pathway are unclear in the context of cetuximab therapy ...


Dynamics Of Bed Bug Infestations And Control Under Disclosure Policies, Sherrie Xie, Alison L. Hill, Chris R. Rehmann, Michael Z. Levy Jan 2019

Dynamics Of Bed Bug Infestations And Control Under Disclosure Policies, Sherrie Xie, Alison L. Hill, Chris R. Rehmann, Michael Z. Levy

Civil, Construction and Environmental Engineering Publications

Bed bugs have reemerged in the United States and worldwide over recent decades, presenting a major challenge to both public health practitioners and housing authorities. A number of municipalities have proposed or initiated policies to stem the bed bug epidemic, but little guidance is available to evaluate them. One contentious policy is disclosure, whereby landlords are obligated to notify potential tenants of current or prior bed bug infestations. Aimed to protect tenants from leasing an infested rental unit, disclosure also creates a kind of quarantine, partially and temporarily removing infested units from the market. Here, we develop a mathematical model ...


Cancer Immunoimaging With Smart Nanoparticles, Yu-Chuan Ou, Xiaona Wen, Rizia Bardhan Jan 2019

Cancer Immunoimaging With Smart Nanoparticles, Yu-Chuan Ou, Xiaona Wen, Rizia Bardhan

Chemical and Biological Engineering Publications

Dynamic immunoimaging in vivo is crucial in patient-tailored immunotherapies to identify patients who will benefit from immunotherapies, monitor therapeutic efficacy post treatment, and determine alternative strategies for nonresponders. Nanoparticles have played a major role in the immunotherapy landscape. In this review, we summarize recent findings in immunoimaging where smart nanoparticles target, detect, stimulate, and deliver therapeutic dose in vivo. Nanoparticles interfaced with an immunoimaging toolbox enable the use of multiple modalities and achieve depth-resolved whole-body tracking of immunomarkers with high accuracy both before and after treatment. We highlight how functional nanoparticles track T cells, dendritic cells (DCs), tumor-associated macrophages (TAMs ...


Single Dose Combination Nanovaccine Provides Protection Against Influenza A Virus In Young And Aged Mice, Kathleen A. Ross, Sujata Senapati, Jessica Alley, Ross Darling, Jonathan Goodman, Matthew Jefferson, Metin Uz, Baoqing Guo, Kyoung-Jin Yoon, David Verhoeven, Marian Kohut, Surya Mallapragada, Michael Wannemuehler, Balaji Narasimhan Jan 2019

Single Dose Combination Nanovaccine Provides Protection Against Influenza A Virus In Young And Aged Mice, Kathleen A. Ross, Sujata Senapati, Jessica Alley, Ross Darling, Jonathan Goodman, Matthew Jefferson, Metin Uz, Baoqing Guo, Kyoung-Jin Yoon, David Verhoeven, Marian Kohut, Surya Mallapragada, Michael Wannemuehler, Balaji Narasimhan

Chemical and Biological Engineering Publications

Immunosenescence poses a formidable challenge in designing effective influenza vaccines for aging populations. While approved vaccines against influenza viruses exist, their efficacy in older adults is significantly decreased due to the diminished capabilities of innate and adaptive immune responses. In this work, the ability of a combination nanovaccine containing both recombinant hemagglutinin and nucleoprotein to provide protection against seasonal influenza virus infection was examined in young and aged mice. Vaccine formulations combining two nanoadjuvants, polyanhydride nanoparticles and pentablock copolymer micelles, were shown to enhance protection against challenge compared to each adjuvant alone in young mice. Nanoparticles were shown to enhance ...