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Full-Text Articles in Engineering
PdmsStar-Peg Hydrogels Prepared Via Solvent-Induced Phase Separation (Sips) And Their Potential Utility As Tissue Engineering Scaffolds, B. M. Bailey, R. Fei, Dany J. Munoz Pinto, M. S. Hahn, M. A. Grunlan
PdmsStar-Peg Hydrogels Prepared Via Solvent-Induced Phase Separation (Sips) And Their Potential Utility As Tissue Engineering Scaffolds, B. M. Bailey, R. Fei, Dany J. Munoz Pinto, M. S. Hahn, M. A. Grunlan
Dany J. Munoz Pinto
Inorganic-organic hydrogels based on methacrylated star polydimethylsiloxane (PDMSstar-MA) and diacrylated poly(ethylene glycol) (PEG-DA) macromers were prepared via solvent-induced phase separation (SIPS). The macromers were combined in a dichloromethane precursor solution and sequentially photopolymerized, dried and hydrated. The chemical and physical properties of the hydrogels were further tailored by varying the number average molecular weight (Mn) of PEG-DA (Mn = 3.4k and 6k g mol-1) as well as the weight percent ratio of PDMSstar-MA (Mn = 7k g mol-1) to PEG-DA from 0:100 to 20:80. Compared to analogous hydrogels …
Collagen-Mimetic Hydrogels Promote Human Endothelial Cell Adhesion, Migration And Phenotypic Maturation, Dany J. Munoz Pinto, V. R. Guiza-Arguello, S. M. Becerra-Bayona, J. Erndt-Marino, S. Samavedi, S. Malmut, B. Russell, M. Höök, M. S. Hahn
Collagen-Mimetic Hydrogels Promote Human Endothelial Cell Adhesion, Migration And Phenotypic Maturation, Dany J. Munoz Pinto, V. R. Guiza-Arguello, S. M. Becerra-Bayona, J. Erndt-Marino, S. Samavedi, S. Malmut, B. Russell, M. Höök, M. S. Hahn
Dany J. Munoz Pinto
This work evaluates the response of human aortic endothelial cells (HAECs) to thromboresistant collagen-mimetic hydrogel coatings toward improving the biocompatibility of existing "off-the-shelf" small-caliber vascular grafts. Specifically, bioactive hydrogels-previously shown to support α1/α2 integrin-mediated cell adhesion but to resist platelet activation-were fabricated by combining poly(ethylene glycol) (PEG) with a 120 kDa, triple-helical collagen-mimetic protein (Scl2-2) containing the GFPGER adhesion sequence. Analysis of HAECs seeded onto the resulting PEG-Scl2-2 hydrogels demonstrated that HAEC adhesion increased with increasing Scl2-2 concentration, while HAEC migration rate decreased over this same concentration range. In addition, evaluation of HAEC phenotype at confluence indicated significant differences in …
Characterization Of Sequential Collagen-Poly(Ethylene Glycol) Diacrylate Interpenetrating Networks And Initial Assessment Of Their Potential For Vascular Tissue Engineering, Dany J. Munoz Pinto, Andrea C. Jimenez-Vergara, T. P. Gharat, M. S. Hahn
Characterization Of Sequential Collagen-Poly(Ethylene Glycol) Diacrylate Interpenetrating Networks And Initial Assessment Of Their Potential For Vascular Tissue Engineering, Dany J. Munoz Pinto, Andrea C. Jimenez-Vergara, T. P. Gharat, M. S. Hahn
Dany J. Munoz Pinto
Collagen hydrogels have been widely investigated as scaffolds for vascular tissue engineering due in part to the capacity of collagen to promote robust cell adhesion and elongation. However, collagen hydrogels display relatively low stiffness and strength, are thrombogenic, and are highly susceptible to cell-mediated contraction. In the current work, we develop and characterize a sequentially-formed interpenetrating network (IPN) that retains the benefits of collagen, but which displays enhanced mechanical stiffness and strength, improved thromboresistance, high physical stability and resistance to contraction. In this strategy, we first form a collagen hydrogel, infuse this hydrogel with poly(ethylene glycol) diacrylate (PEGDA), and subsequently …