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Full-Text Articles in Engineering

Tailoring Cell-Material Interactions Via Poly(Acrylic Acid) Brushes To Enhance Nonviral Substrate-Mediated Gene Delivery, Amy Mantz Jul 2019

Tailoring Cell-Material Interactions Via Poly(Acrylic Acid) Brushes To Enhance Nonviral Substrate-Mediated Gene Delivery, Amy Mantz

Biological Systems Engineering--Dissertations, Theses, and Student Research

Nonviral gene delivery modifies gene expression by transferring exogenous genetic material into cells and tissues, typically through a bolus of complexes formed by electrostatic interactions between cationic lipid or polymer vectors with negatively charged nucleic acids (e.g. DNA). Although nonviral gene delivery is safer, more cost-effective, and more flexible compared to viral systems, nonviral transfection suffers from low efficiency due to extracellular and intracellular barriers. Much research has focused on tuning physiochemical properties of the complexing vectors to improve transfection, yet the cell-material interface may prove a better platform to immobilize DNA complexes for substrate-mediated delivery (SMD) and modulate ...


Nucleic Acid Delivery To Mesenchymal Stem Cells: A Review Of Nonviral Methods And Applications, Andrew Hamann, Albert Nguyen, Angela K. Pannier Jan 2019

Nucleic Acid Delivery To Mesenchymal Stem Cells: A Review Of Nonviral Methods And Applications, Andrew Hamann, Albert Nguyen, Angela K. Pannier

Biological Systems Engineering: Papers and Publications

Background: Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated and expanded from many tissues, and are being investigated for use in cell therapies. Though MSC therapies have demonstrated some success, none have been FDA approved for clinical use. MSCs lose stemness ex vivo, decreasing therapeutic potential, and face additional barriers in vivo, decreasing therapeutic efficacy. Culture optimization and genetic modification of MSCs can overcome these barriers. Viral transduction is efficient, but limited by safety concerns related to mutagenicity of integrating viral vectors and potential immunogenicity of viral antigens. Nonviral delivery methods are safer, though limited by ...


Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery On Titanium Substrates Modified With Rgd-Functionalized Poly(Acrylic Acid) Brushes, Amy Mantz, Alice Rosenthal, Eric Farris, Tyler Kozisek, Eva Bittrich, Saghar Nazari, Eva Schubert, Mathias Schubert, Manfred Stamm, Petra Uhlmann, Angela K. Pannier Jan 2019

Free Polyethylenimine Enhances Substrate-Mediated Gene Delivery On Titanium Substrates Modified With Rgd-Functionalized Poly(Acrylic Acid) Brushes, Amy Mantz, Alice Rosenthal, Eric Farris, Tyler Kozisek, Eva Bittrich, Saghar Nazari, Eva Schubert, Mathias Schubert, Manfred Stamm, Petra Uhlmann, Angela K. Pannier

Biological Systems Engineering: Papers and Publications

Substrate mediated gene delivery (SMD) is a method of immobilizing DNA complexes to a substrate via covalent attachment or nonspecific adsorption, which allows for increased transgene expression with less DNA compared to traditional bolus delivery. It may also increase cells receptivity to transfection via cell-material interactions. Substrate modifications with poly(acrylic) acid (PAA) brushes may improve SMD by enhancing substrate interactions with DNA complexes via tailored surface chemistry and increasing cellular adhesion via moieties covalently bound to the brushes. Previously, we described a simple method to graft PAA brushes to Ti and further demonstrated conjugation of cell adhesion peptides (i ...