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Biomaterial Substrate Modifications That Influence Cell-Material Interactions To Prime Cellular Responses To Nonviral Gene Delivery, Amy Mantz, Angela K. Pannier
Biomaterial Substrate Modifications That Influence Cell-Material Interactions To Prime Cellular Responses To Nonviral Gene Delivery, Amy Mantz, Angela K. Pannier
Biological Systems Engineering: Papers and Publications
Gene delivery is the transfer of exogenous genetic material into somatic cells to modify their gene expression, with applications including tissue engineering, regenerative medicine, sensors and diagnostics, and gene therapy. Viral vectors are considered the most effective system to deliver nucleic acids, yet safety concerns and many other disadvantages have resulted in investigations into an alternative option, i.e. nonviral gene delivery. Chemical nonviral gene delivery is typically accomplished by electrostatically complexing cationic lipids or polymers with negatively charged nucleic acids. Unfortunately, nonviral gene delivery suffers from low efficiency due to barriers that impede transfection success, including intracellular processes such as …
Nucleic Acid Delivery To Mesenchymal Stem Cells: A Review Of Nonviral Methods And Applications, Andrew Hamann, Albert Nguyen, Angela K. Pannier
Nucleic Acid Delivery To Mesenchymal Stem Cells: A Review Of Nonviral Methods And Applications, Andrew Hamann, Albert Nguyen, Angela K. Pannier
Biological Systems Engineering: Papers and Publications
Background: Mesenchymal stem cells (MSCs) are multipotent stem cells that can be isolated and expanded from many tissues, and are being investigated for use in cell therapies. Though MSC therapies have demonstrated some success, none have been FDA approved for clinical use. MSCs lose stemness ex vivo, decreasing therapeutic potential, and face additional barriers in vivo, decreasing therapeutic efficacy. Culture optimization and genetic modification of MSCs can overcome these barriers. Viral transduction is efficient, but limited by safety concerns related to mutagenicity of integrating viral vectors and potential immunogenicity of viral antigens. Nonviral delivery methods are safer, though limited …