Open Access. Powered by Scholars. Published by Universities.®

Engineering Commons

Open Access. Powered by Scholars. Published by Universities.®

2019

University of Arkansas, Fayetteville

Discipline
Keyword
Publication
Publication Type

Articles 31 - 60 of 184

Full-Text Articles in Engineering

Evaluation And Analysis Of Null Convention Logic Circuits, John Davis Brady Dec 2019

Evaluation And Analysis Of Null Convention Logic Circuits, John Davis Brady

Graduate Theses and Dissertations

Integrated circuit (IC) designers face many challenges in utilizing state-of-the-art technology nodes, such as the increased effects of process variation on timing analysis and heterogeneous multi-die architectures that span across multiple technologies while simultaneously increasing performance and decreasing power consumption. These challenges provide opportunity for utilization of asynchronous design paradigms due to their inherent flexibility and robustness.

While NULL Convention Logic (NCL) has been implemented in a variety of applications, current literature does not fully encompass the intricacies of NCL power performance across a variety of applications, technology nodes, circuit scale, and voltage scaling, thereby preventing further adoption and utilization …


Image-Driven Automated End-To-End Testing For Mobile Applications, Caleb Fritz Dec 2019

Image-Driven Automated End-To-End Testing For Mobile Applications, Caleb Fritz

Computer Science and Computer Engineering Undergraduate Honors Theses

The increasing complexity and demand of software systems and the greater availability of test automation software is quickly rendering manual end-to-end (E2E) testing techniques for mobile platforms obsolete. This research seeks to explore the potential increase in automated test efficacy and maintainability through the use of computer vision algorithms when applied with Appium, a leading cross-platform mobile test automation framework. A testing framework written in a Node.js environment was created to support the development of E2E test scripts that examine and report the functional capabilities of a mobile test app. The test framework provides a suite of functions that connect …


Silver-Based Microbial Check Valve For Spacecraft Potable Water Systems, Tanner Burns Dec 2019

Silver-Based Microbial Check Valve For Spacecraft Potable Water Systems, Tanner Burns

Chemical Engineering Undergraduate Honors Theses

As human space exploration increases, the development of a more efficient potable water treatment system suited for spacecraft becomes crucial. This Waste-management Education Research Consortium (WERC) challenge was designed to explore the viability of microbial control through the utilization of silver ions as a biocide for possible integration into the Tranquility Node 3 water purification system aboard the International Space Station (ISS). Current systems using iodine risk causing hyperthyroidism from overexposure; however, silver can be safely ingested without this side effect. After researching silver delivery methods including electrochemical ion production, controlled release, or a combination of the two, our team …


Hvac System Energy Audit For Leverett Elementary School, Connor Smalling Dec 2019

Hvac System Energy Audit For Leverett Elementary School, Connor Smalling

Biological and Agricultural Engineering Undergraduate Honors Theses

Leverett Elementary School is located in Fayetteville, AR. The school needs significant upgrades to its infrastructure. The Fayetteville Public School District has voted to pursue an Energy Services Performance Contract (ESPC) in order to finance the desired upgrades to Leverett Elementary, among other schools in the district.

The scope of this thesis was to perform an energy audit on the existing heating, ventilation, and air conditioning (HVAC) system. By using an energy modeling software, eQuest, the building and the existing base system were modeled to determine utility consumption. Three different HVAC system alternatives were analyzed against the base system by …


Drivers Of On-Farm Performance Of Irrigation Water Management Practices: Empirical Evidence From Eastern Arkansas, Jacob Askey Dec 2019

Drivers Of On-Farm Performance Of Irrigation Water Management Practices: Empirical Evidence From Eastern Arkansas, Jacob Askey

Biological and Agricultural Engineering Undergraduate Honors Theses

Unsustainable agricultural practices are putting a strain on freshwater supplies in many parts of the world. More efficient irrigation techniques are going to be critical to feed a growing population. Data from the 2016 Arkansas Irrigation Survey was used to assess the adoption of three Irrigation Water Management (IWM) practices: multiple inlet rice irrigation, tailwater recovery, and storage reservoirs. Results indicate that these practices do not always lead to reported pumping time reductions, a proxy for water use savings. Large variations in pumping time reduction are observed across producers for all three practices. A Tobit model was used to examine …


Optical Spectrsocopy Of Murine Breast Tumor To Distinguish Indolent From Aggressive Disease, Joel Rodriguez Troncoso Dec 2019

Optical Spectrsocopy Of Murine Breast Tumor To Distinguish Indolent From Aggressive Disease, Joel Rodriguez Troncoso

Biomedical Engineering Undergraduate Honors Theses

Breast cancer accounts for 30% of all cancer. Metastasis is the primary cause of death among breast cancer patients. Additionally, current molecular profiling methods such as Oncotype DX, which are expensive and not widely available at all clinical facilities, only determine the risk of recurrence after treatment. Therefore, there are no current method capable of identifying metastatic patients in advance.As a result, there is an unmet clinical need to develop a cost-effective prognostic to differentiate between indolent and aggressive breast tumors. In this study, we implemented diffuse reflectance spectroscopy (DRS) system to evaluate functional changes in tumor xenografts originated from …


Ultrasonic Wave Propagation In Copper/Graphene Metal Matrix Composites, Casey Lindbloom Dec 2019

Ultrasonic Wave Propagation In Copper/Graphene Metal Matrix Composites, Casey Lindbloom

Mechanical Engineering Undergraduate Honors Theses

Emerging metallic composite materials implanted with graphene sheets are showing immense promise, with benefits being observed with regards to mechanical, thermal, and electrical material properties. This research aims to investigate the effects on ultrasonic wave propagation in Copper/Graphene Metal Matrix Composites (Cu/Gr MMCs) with varying graphene arrangements inspired from nacre and bone nanoscale material distributions. To accomplish this, the molecular dynamics (MD) method is utilized to simulate nanoscale wave propagation on a set of Cu/Gr MMCs with differing graphene arrangements and volume percentages ranging up to 4.56%. The computational model results are then analyzed to determine the variation in energy …


Fractional Order State Feedback Control For Improved Lateral Stability Of Semi-Autonomous Commercial Heavy Vehicles, Rasheed Mazen Abdulkader Dec 2019

Fractional Order State Feedback Control For Improved Lateral Stability Of Semi-Autonomous Commercial Heavy Vehicles, Rasheed Mazen Abdulkader

Graduate Theses and Dissertations

With the growing development of autonomous and semi-autonomous large commercial heavy vehicles, the lateral stability control of articulated vehicles have caught the attention of researchers recently. Active vehicle front steering (AFS) can enhance the handling performance and stability of articulated vehicles for an emergency highway maneuver scenario. However, with large vehicles such tractor-trailers, the system becomes more complex to control and there is an increased occurrence of instabilities. This research investigates a new control scheme based on fractional calculus as a technique that ensures lateral stability of articulated large heavy vehicles during evasive highway maneuvering scenarios. The control method is …


Nematode Populations As Affected By Residue And Water Management In A Long-Term Wheat-Soybean Double Crop In Eastern Arkansas, Lucia Emperatriz Escalante Ortiz Dec 2019

Nematode Populations As Affected By Residue And Water Management In A Long-Term Wheat-Soybean Double Crop In Eastern Arkansas, Lucia Emperatriz Escalante Ortiz

Graduate Theses and Dissertations

Soybeans (Glycine max) are one of the major row crops in the United States, particularly in Arkansas. Soybean cyst nematode (SCN, Heterodera glycines) and southern root-knot nematode (RKN, Meloidogyne incognita) are two of the most damaging pests that cause major economic losses in soybeans. Little is known concerning the effects of common and alternative agronomic practices on nematodes in fields with nematode population densities below threshold levels. Therefore, the objective of this study was to evaluate the effects of the combination of tillage (conventional tillage and no-tillage), irrigation (irrigated and non-irrigated), wheat (Triticum aestivum) residue burning (burned and no burned), …


Synthesis Of Cellulose Nanocrystal-Gold Nanoparticle Hybrid System For Surface Plasmon-Enhanced Property, Mahshid Iraniparast Dec 2019

Synthesis Of Cellulose Nanocrystal-Gold Nanoparticle Hybrid System For Surface Plasmon-Enhanced Property, Mahshid Iraniparast

Graduate Theses and Dissertations

Gold nanoparticles (AuNPs) have been brought to the forefront of various applications, ranging from theranostics, to organic photovoltaics, to biosensing owing to their localized surface plasmon resonance (LSPR) property. However, this property needs to be improved in order to allow for high sensitivity and quantitative detection of biomolecules. Hybrids of AuNPs with low-dielectric cellulose nanocrystal (CNCs) would yield enhancement of the LSPR property, which is driven by the confinement of electron oscillation at their interfaces. This study proposed a seed-mediated growth method to synthesize hybrids of CNCs-AuNPs. Sulfate groups on the surface of CNCs served as the sites for the …


Studies Of Initial Growth Of Gan On Inn, Alaa Alnami Dec 2019

Studies Of Initial Growth Of Gan On Inn, Alaa Alnami

Graduate Theses and Dissertations

III-nitride materials have recently attracted much attention for applications in both the microelectronics and optoelectronics. For optoelectronic devices, III-nitride materials with tunable energy band gaps can be used as the active region of devices to enhance the absorption or emission. A such material is indium nitride (InN), which along with gallium nitride (GaN) and aluminum nitride (AlN) embody the very real promise of forming the basis of a broad spectrum, a high efficiency solar cell. One of the remaining complications in incorporating InN into a solar cell design is the effects of the high temperature growth of the GaN crystal …


Development Of Forward Osmosis Based Separations: A Novel Approach In Membrane Technology, Yu-Hsuan Chiao Dec 2019

Development Of Forward Osmosis Based Separations: A Novel Approach In Membrane Technology, Yu-Hsuan Chiao

Graduate Theses and Dissertations

Tackling the worldwide severe water shortage, the membrane technology is considered to be the most efficient approach and hence, used widely as a cost-effective sustainable solution. “Forward osmosis (FO)” has been the major attention in recent time. FO uses osmotic pressure as the driving force to draw the water passing the membrane and achieve the desired separation performance. In general, it is considered to be a process with tremendous potential to resolve the present-day water shortage with extremely low energy consumption. However, the challenges of membrane and draw solution regeneration associated with FO processes must be conquered prior to their …


Axial Compressor Based On Plastic Additive Manufacturing, Chris Phu Dec 2019

Axial Compressor Based On Plastic Additive Manufacturing, Chris Phu

Mechanical Engineering Undergraduate Honors Theses

Turbomolecular pumps designed to function in very low pressures tend to be too prohibitively expensive for student researchers. On the other hand, while conventional pumps are affordable, they can’t function in extreme low pressures. An additively manufactured axial compressor however is inexpensive to manufacturer and only needs to be build up enough pressure for a conventional pump to function. After many design iterations, a final iteration that is near vacuum chamber ready has been 3D printed and tested for spin functionally. The designed axial compressor is easy to assemble and very modular. Conclusions from each of the design iterations is …


Optimized Production And Evaluation Of Cellulose Nanocrystals Derived From Pre-Extracted Kraft Pulp Of Different Wood Species, Gurshagan Kandhola Dec 2019

Optimized Production And Evaluation Of Cellulose Nanocrystals Derived From Pre-Extracted Kraft Pulp Of Different Wood Species, Gurshagan Kandhola

Graduate Theses and Dissertations

Production of nanocellulose from a variety of naturally abundant, locally available and industrially significant wood species provides an opportunity for diversifying the portfolio of traditional pulp and paper industries. The U.S. has a prolific forest products industry with a well-established infrastructure that could be utilized for optimized and customized production of cellulose nanomaterials. However, to achieve that, it is important to a) understand how biorefining strategies for complete fractionation of biomass affect the downstream processing of pulp into nanocellulose, b) maximize the yields of cellulose nanocrystals and nanofibers (CNCs and CNFs) from pretreated raw materials, and c) evaluate if the …


Theoretical Investigations Of The Electronic, Magnetic, And Thermoelectric Properties Of Transition-Metal Based Compounds, Haleoot Edaan Raad Dec 2019

Theoretical Investigations Of The Electronic, Magnetic, And Thermoelectric Properties Of Transition-Metal Based Compounds, Haleoot Edaan Raad

Graduate Theses and Dissertations

The electronic, magnetic, and thermoelectric properties of transition-metal based compounds were investigated by using the density functional theory and Boltzmann transport formalism. It was found that the Co-based Heusler compounds and InSe monochalcogenide are among the materials that may be used for future thermoelectric devices. Furthermore, the investigation showed that the quaternary Heusler compounds, such as, CoFeYGe, where Y is Ti or Cr, are half-metallic ferromagnetic materials with full electron spin polarization. The lattice thermal conductivity (κL) was found to decrease for these alloys as the temperature increases. The present investigation indicated that the phonon optical modes have a major …


Microextrusion 3d Printing Of Optical Waveguides And Microheaters, Edidiong Nseowo Udofia Aug 2019

Microextrusion 3d Printing Of Optical Waveguides And Microheaters, Edidiong Nseowo Udofia

Graduate Theses and Dissertations

The drive for smaller and more compact devices presents several challenges in materials and fabrication strategies. Although photolithography is a well-developed method for creating microdevices, the disparate requirements in fabrication strategies, material choices, equipment and process complexities have limited its applications. Microextrusion printing (μEP) provides a promising alternative for microfabrication. Compared to the traditional techniques, the attractions lie in the wide range of printable material choice, greater design freedom, fewer processing steps, lower cost for customized production, and the plurality of compatible substrates. However, while extrusion-based 3D printing processes have been successfully applied at the macroscale, this seeming simplicity belies …


The Development Of Initial Asphalt Mixture Acceptance Criteria Using The Scb Test And The I-Fit Test, Nathan Delman Parnell Aug 2019

The Development Of Initial Asphalt Mixture Acceptance Criteria Using The Scb Test And The I-Fit Test, Nathan Delman Parnell

Graduate Theses and Dissertations

Cracking has become the primary asphalt pavement distress in Arkansas. Arkansas along with other states have implemented a test to measure rutting resistance. This paper presents research to implement a cracking resistance test to further modify the Superpave volumetric asphalt mixture design method in Arkansas. The Illinois Flexibility Index Test (I-FIT) was the test chosen to analyze for this research. Two aging methods were tested, a short term oven aging (AASHTO R30 ST) and a long term oven aging (NCAT) method. Six asphalt mixtures were selected to be tested. The mixtures testes were recreated from Arkansas Department of Transportation (ARDOT) …


Toolpath Planning Methodology For Multi-Gantry Fused Filament Fabrication 3d Printing, Hieu Trung Bui Aug 2019

Toolpath Planning Methodology For Multi-Gantry Fused Filament Fabrication 3d Printing, Hieu Trung Bui

Graduate Theses and Dissertations

Additive manufacturing (AM) has revolutionized the way industries manufacture and prototype products. Fused filament fabrication (FFF) is one of the most popular processes in AM as it is inexpensive, requires low maintenance, and has high material utilization. However, the biggest drawback that prevents FFF printing from being widely implemented in large-scale production is the cycle time. The most practical approach is to allow multiple collaborating printheads to work simultaneously on different parts of the same object. However, little research has been introduced to support the aforementioned approach. Hence a new toolpath planning methodology is proposed in this paper. The objectives …


Five-Level Flying Capacitor Converter Used As A Static Compensator For Current Unbalances In Three-Phase Distribution Systems, Rafael Franceschi Aug 2019

Five-Level Flying Capacitor Converter Used As A Static Compensator For Current Unbalances In Three-Phase Distribution Systems, Rafael Franceschi

Graduate Theses and Dissertations

This thesis presents and evaluates a solution for unbalanced current loading in three-phase distribution systems. The proposed solution uses the flying capacitor multilevel converter as its main topology for an application known as Unbalanced Current Static Compensator. The fundamental theory, controller design and prototype construction will be presented along with the experimental results. The Unbalanced Current Static Compensator main objective is the balancing of the up-stream currents from the installation point to eliminate the negative- and zero-sequence currents originated by unbalanced single-phase loads.

Three separate single-phase flying capacitor converters are controlled independently using a d-q rotating reference frame algorithm to …


Modification And Optimization Of Conducting Polymer-Modified, Redox-Magnetohydrodynamics (R-Mhd) Pumping For Enhanced And Sustained Microfluidics Applications, Md Foysal Zahid Khan Aug 2019

Modification And Optimization Of Conducting Polymer-Modified, Redox-Magnetohydrodynamics (R-Mhd) Pumping For Enhanced And Sustained Microfluidics Applications, Md Foysal Zahid Khan

Graduate Theses and Dissertations

In this work, a novel microfluidic pumping approach, redox-magnetohydrodynamics (R-MHD) has improved by materials and device optimization to use in lab-on-a-chip applications. In R-MHD, magnetic flux (B) and ionic current density (j) interacts to generate body force (FB) in between active electrodes, according to the equation FB = j×B. This unique fluid pumping approach is scalable, tunable, generates flat flow profile, and does not require any channels or valves. Pumping performance, such as speed scales with the ionic current density (j) and duration depends on the total charge (Q). The ionic current density (j) results from the conversion of electronic …


Development Of A Multimode Instrument For Remote Measurements Of Unsaturated Soil Properties, Sean Elliott Salazar Aug 2019

Development Of A Multimode Instrument For Remote Measurements Of Unsaturated Soil Properties, Sean Elliott Salazar

Graduate Theses and Dissertations

The hydromechanical behavior of soil is governed by parameters that include the moisture content, soil matric potential, texture, and the mineralogical composition of the soil. Remote characterization of these and other key properties of the soil offers advantages over conventional in situ or laboratory-based measurements: information may be acquired rapidly over large, or inaccessible areas; samples do not need to be collected; and the measurements are non-destructive. A field-deployable, ground-based remote sensor, designated the Soil Observation Laser Absorption Spectrometer (SOLAS), was developed to infer parameters of bare soils and other natural surfaces over intermediate (100 m) and long (1,000 m) …


Switching Trajectory Control For High Voltage Silicon Carbide Power Devices With Novel Active Gate Drivers, Shuang Zhao Aug 2019

Switching Trajectory Control For High Voltage Silicon Carbide Power Devices With Novel Active Gate Drivers, Shuang Zhao

Graduate Theses and Dissertations

The penetration of silicon carbide (SiC) semiconductor devices is increasing in the power industry due to their lower parasitics, higher blocking voltage, and higher thermal conductivity over their silicon (Si) counterparts. Applications of high voltage SiC power devices, generally 10 kV or higher, can significantly reduce the amount of the cascaded levels of converters in the distributed system, simplify the system by reducing the number of the semiconductor devices, and increase the system reliability.

However, the gate drivers for high voltage SiC devices are not available on the market. Also, the characteristics of the third generation 10 kV SiC MOSFETs …


Growth Of Indium Nitride Quantum Dots By Molecular Beam Epitaxy, Steven P. Minor Aug 2019

Growth Of Indium Nitride Quantum Dots By Molecular Beam Epitaxy, Steven P. Minor

Graduate Theses and Dissertations

Over the last decade, the evolution of the global consciousness in response to decreasing environmental conditions from global warming and pollution has led to an outcry for finding new alternative/clean methods for harvesting energy and determining ways to minimize energy consumption. III-nitride materials are of interest for optoelectronic and electronic device applications such as high efficiency solar cells, solid state lighting (LEDs), and blue laser (Blu-ray Technology) applications. The wide range of direct band gaps covered by its alloys (0.7eV-6.2eV) best illustrates the versatility of III-nitride materials. This wide range has enabled applications extending from the ultraviolet to the near …


Investigation Of The Effect Of Age On Regenerative Outcomes Following Treatment Of Volumetric Muscle Loss Injuries, John Taehwan Kim Aug 2019

Investigation Of The Effect Of Age On Regenerative Outcomes Following Treatment Of Volumetric Muscle Loss Injuries, John Taehwan Kim

Graduate Theses and Dissertations

Volumetric muscle loss (VML) is a traumatic injury in skeletal muscle resulting in the bulk loss of more than 20% of the muscle’s volume. Included in the bulk loss of muscle is the skeletal muscle niche comprised of nerve bundles, vasculature, local progenitor cells, basal lamina, and muscle fibers, overwhelming innate repair mechanisms. The hallmark of VML injury is the excessive accumulation of non-contractile, fibrotic tissue and permanent functional deficits. Though predominant in the younger demographic, the elderly population is also captured within VML injuries. There are many factors that change with aging in skeletal muscle that may further hinder …


Polarization Properties Of Airy And Ince-Gaussian Laser Beams, Sean Michael Nomoto Aug 2019

Polarization Properties Of Airy And Ince-Gaussian Laser Beams, Sean Michael Nomoto

Graduate Theses and Dissertations

The description of polarization states of laser light as linear, circular polarization within the paraxial scalar wave approximation is adequate for most applications. However, this description falls short when considering laser light as an electromagnetic wave satisfying Maxwell's equations. An electric field with a constant unit vector for direction of the field and a space dependent complex scalar amplitude in the paraxial wave approximation does not satisfy Maxwell equations which, in general, requires all three Cartesian components of electric and magnetic fields associated for a nonzero laser beam to be nonzero.

Physical observation of passing a linearly polarized laser through …


A Simulation Tool For Evaluating The Environmental Impacts Of Management Scenarios For Modern Broiler Production Systems, Martin Andrew Christie Aug 2019

A Simulation Tool For Evaluating The Environmental Impacts Of Management Scenarios For Modern Broiler Production Systems, Martin Andrew Christie

Graduate Theses and Dissertations

The purpose of this work is to provide a simulation tool that allows broiler production practitioners and researchers to simulate the effects of farm design and management practices on resource consumption and environmental impacts. This tool allows the user to design unique farms and simulates on farm processes required to raise broiler chicks to a marketable age. The use can input data such as farm location, broiler breed, flock size, ration type, barn dimensions, and climate control equipment specifications. The algorithms used to simulate broiler breed specific feed intake, broiler weight gain, and other on farm processes such as heating, …


A Study Of Protein And Peptide-Directed Nanoparticle Synthesis For Catalytic Materials, Abdollah Mosleh Aug 2019

A Study Of Protein And Peptide-Directed Nanoparticle Synthesis For Catalytic Materials, Abdollah Mosleh

Graduate Theses and Dissertations

Nanoparticles have received much attentions due to their unique properties that makes them suitable candidates for a broad range of applications. As the size of particles decreases, their surface area-to-volume ratio would increase which is the main cause of much attention. In addition to the size, their morphologies and compositions may also play important roles for defining unique properties. Nanoparticle synthesis include both bottom-up and top-down strategies. To control the process of inorganic nanoparticles synthesis one could follow the bottom-up approach to have atom-level control over their compositions, morphologies, phases, and sizes which is the subject of this work. Due …


Displacement Derived Energy Levels For Harvard Miniature Compaction, Ana Laura Errigo, Julia Loshelder Aug 2019

Displacement Derived Energy Levels For Harvard Miniature Compaction, Ana Laura Errigo, Julia Loshelder

Civil Engineering Undergraduate Honors Theses

Previously, the amount of energy provided by the Harvard Miniature tamper was adjusted by changing the number of blows per lift or the number of lifts or was not adjusted at all. However, this research was conducted to investigate the effect of adjusting the displacement of the spring (increased or decreased) to change the potential energy of the spring and therefore change the compacive effort with a specific type of soil. Thus, the objective was to match the compaction energies using the Harvard miniature method with the compaction energies obtained using the Proctor method. Specifically, the goal was to show …


Development Of A Ground-Based Aerial-Tracking Instrument For Open-Path Spectroscopy To Monitor Atmospheric Constituents, Haden Hodges Aug 2019

Development Of A Ground-Based Aerial-Tracking Instrument For Open-Path Spectroscopy To Monitor Atmospheric Constituents, Haden Hodges

Civil Engineering Undergraduate Honors Theses

A ground-based aerial-tracking instrument, known as the Ground Tracker, designed to provide spectral data to quantify greenhouse gases is under development. The Ground Tracker includes an Optical System including a high power rifle scope, video camera, and spectrometer used to locate an active light source from the Emitter, and collect spectral data by utilizing an actuating mirror. The implementation of this instrument could be made low cost by utilizing existing weather balloon infrastructure to allow the Emitter to be placed into the lower stratosphere. The recovery of the emitter will be possible by tracking the GPS coordinates. Weather balloon instrument …


Generation Of A Ccl2 Knockout Using Crispr/Cas9 And Lipid Mediated Transfection In Ct-26 Murine Colon Carcinoma Cells, Emma Sullivan Aug 2019

Generation Of A Ccl2 Knockout Using Crispr/Cas9 And Lipid Mediated Transfection In Ct-26 Murine Colon Carcinoma Cells, Emma Sullivan

Biomedical Engineering Undergraduate Honors Theses

CCL2 is an inflammatory mediator that is released by tumor cells to activate and direct immune cell species, especially macrophages, to inflammatory sites within the body. The goal of this project was to successfully generate knockout the CCL2 ligand gene using a CRISPR/Cas9 complex delivered via lipid mediated transfection. The sgRNA and Cas9 mRNA were introduced into the cells via lipid-mediated transfection. The cells were incubated for 4 days, before being analyzed using PCR and gel electrophoresis. We expected to see one band on the first gel and two bands on the second gel. Two bands appeared on the first …