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Full-Text Articles in Engineering

The Effect Of Heterobifunctional Crosslinkers On Hema Hydrogel Modulus And Toughness, Elizabeth M. Boazak, Vaughn K. Greene Jr., Debra T. Auguste May 2019

The Effect Of Heterobifunctional Crosslinkers On Hema Hydrogel Modulus And Toughness, Elizabeth M. Boazak, Vaughn K. Greene Jr., Debra T. Auguste

Publications and Research

The use of hydrogels in load bearing applications is often limited by insufficient toughness. 2-Hydroxyethyl methacrylate (HEMA) based hydrogels are appealing for translational work, as they are affordable and the use of HEMA is FDA approved. Furthermore, HEMA is photopolymerizable, providing spatiotemporal control over mechanical properties. We evaluated the ability of vinyl methacrylate (VM), allyl methacrylate (AM), and 3-(Acryloyloxy)-2-hydroxypropyl methacrylate (AHPM) to tune hydrogel toughness and Young’s modulus. The crosslinkers were selected due to their heterobifunctionality (vinyl and methacrylate) and similar size and structure to EGDMA, which was shown previously to increase toughness as compared to longer ...


Modification Of Lipid Microenvironments On Solid Support Structures For Use In Transmembrane Protein Assays, William J. Houlihan Jan 2019

Modification Of Lipid Microenvironments On Solid Support Structures For Use In Transmembrane Protein Assays, William J. Houlihan

Dissertations and Theses

Gamma-Secretase (γ-secretase) is a transmembrane protease of increasing interest, which has been shown to have significant connections to both cancer and Alzheimer’s disease. γ-secretase cleaves both Notch-1, a transmembrane signaling protein, and Amyloid precursor protein (APP), a transmembrane protein whose cleavage may result in the formation of β-amyloid plaques in the brain. Notch-1 and APP are widely studied proteins that have substantial impacts on the development and proliferation of cancer and Alzheimer’s disease, respectively. Notch-1 partakes in the signaling of apoptosis in damaged and mutated cells, thus its cleavage by γ-secretase within the plasma membrane has ramifications on ...


Controlled Migration Of Retinal Progenitor Cells Within Electro-Chemotactic Fields, Shawn Mishra Jan 2019

Controlled Migration Of Retinal Progenitor Cells Within Electro-Chemotactic Fields, Shawn Mishra

Dissertations and Theses

Vision loss in retinal degenerative diseases is overwhelmingly attributed to damage and death of retinal photoreceptor cells. Studies in mouse retina have suggested that transplantation of isolated post-natal or stem cell-derived retinal progenitor cells (RPCs) to replace apoptotic or damaged photoreceptors may be a novel approach to restore vision. Thus far, outcomes project that the amount of restored visual response depends upon the migration of transplanted cells from insertion in the sub-retinal space to the outer nuclear layer (ONL). However, transplantation efficiency is exceedingly low – ~5% cells transplanted enter the retina – directly limiting the efficacy of the treatments. Additionally, the ...