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- Scanning electron microscopy (5)
- Biomaterials (3)
- Biocompatibility (2)
- Bone resorption (2)
- Calcitriol treatment (2)
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- Extracellular matrix (2)
- Hydroxyapatite (2)
- Microstructure (2)
- A-lactalbumin (1)
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- Backscattered electron imaging (1)
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- Bioceramics (1)
- Biodegradable polymer (1)
- Biodegradation (1)
- Biofilms (1)
- Biomaterial (1)
- Biomineralization (1)
- Biphasic calcium phosphate ceramic (1)
- Biphasic effects (1)
- Blood vessel substitute (1)
- Bone cement (1)
- Bone fluoride content (1)
- Bone formation (1)
- Bone histomorphometry (1)
- Bone mineral (1)
Articles 1 - 23 of 23
Full-Text Articles in Engineering
Scanning Electron Microscopy Studies Of Ceramic Layers And Interfacial Regions For Calcium Phosphate-Coated Titanium Dental Implants, W. A. Brantley, E. Tufecki, J. C. Mitchell, D. W. Foreman, E. A. Mcglumphy
Scanning Electron Microscopy Studies Of Ceramic Layers And Interfacial Regions For Calcium Phosphate-Coated Titanium Dental Implants, W. A. Brantley, E. Tufecki, J. C. Mitchell, D. W. Foreman, E. A. Mcglumphy
Cells and Materials
The surface and cross-section characteristics of the plasma-sprayed calcium phosphate coatings, along with the microstructures and elemental compositions near the titanium alloy interface, were investigated by scanning electron microscopy for two different dental implants of proprietary compositions: Integra!® (Calcitek) and BioVent ® (Dentsply). Elemental concentrations (Ca, P, Ti, AI, and V) near the interfaces were obtained by X-ray energy-dispersive spectroscopy. Coating surfaces exhibited a splat deposition topography of greatly differing microstructural scale for the two implants, along with other features characteristic of the plasma-spraying process; cross-sections of the coatings revealed minimal porosity. Some interdiffusion of principally titanium and calcium was …
In Vitro Characterization Of Transforming Growth Factor-Ss1-Loaded Composites Of Biodegradable Polymer And Mesenchymal Cells, S. B. Nicoll, A. E. Denker, R. S. Tuan
In Vitro Characterization Of Transforming Growth Factor-Ss1-Loaded Composites Of Biodegradable Polymer And Mesenchymal Cells, S. B. Nicoll, A. E. Denker, R. S. Tuan
Cells and Materials
A porous, biodegradable polymer composite was fabricated from poly(L-lactic acid) (PLLA) using a solvent- casting particulate-leaching technique, and loaded with recombinant human transforming growth factor ß-1 (TGF-ß1). Tissue culture polystyrene (TCPS) and PLLA disks loaded with bovine serum albumin (BSA) served as controls. Polymer specimens were seeded at high cell density with C3H10T1/2 cells, a mouse embryonic cell line capable of differentiating into various connective tissue cell types. The polymer matrices degraded slowly in physiological saline over four weeks, with a sustained yet sporadic release of active TGF-ß1; a more controlled release of TGF-ß1 was observed in serum-containing culture medium …
Three-Dimensional Morphology And Platelet Adhesion On Pyrolytic Carbon Heart Valve Materials, S. L. Goodman, K. S. Tweden, R. M. Albrecht
Three-Dimensional Morphology And Platelet Adhesion On Pyrolytic Carbon Heart Valve Materials, S. L. Goodman, K. S. Tweden, R. M. Albrecht
Cells and Materials
Low-temperature isotropic pyrolytic carbon (LTIC) is the preferred material for mechanical heart valve prosthetics due to its durability and good thromboresistance, although thromboembolic complications remain a significant clinical problem. LTlC morphology has been previously studied using scanning (SEM) and transmission electron microscopy (TEM), and scanning tunneling microscopy (STM). However, these microscopies have limitations with imaging rough surfaces. In this study, LTIC valve leaflets from CarboMedics, Inc. and St. Jude Medical, Inc. were prepared and polished exactly as used in clinical prosthetics, and examined at magnifications up to macromolecular resolution using stereo-pair low-voltage SEM (LV -SEM). LV -SEM reveals that LTIC …
Adsorption Of Bovine Serum Albumin Onto Octacalcium Phosphate And Its Hydrolyzates, O. Suzuki, H. Yagishita, M. Yamazaki, T. Aoba
Adsorption Of Bovine Serum Albumin Onto Octacalcium Phosphate And Its Hydrolyzates, O. Suzuki, H. Yagishita, M. Yamazaki, T. Aoba
Cells and Materials
Octacalcium phosphate (OCP) has been advocated to be a precursor of biomineral s. Tn this report , we aimed at investigating OCP hydrolysis and the interaction of OCP and its hydrolyzates (apatitic products) with bovine serum albumin (BSA). A batch of synthetic OCP was allowed to hydrolyze either (a) in deionized water at 70°C or (b) in Tris buffer (pH 7.4) containing 2 ppm fluoride at 37°C. OCP hydrolysis was completed within reasonable experimental periods: after 48 hours at 70°C and after 10 days at 37°C in the presence of fluoride. The adsorption isotherms of BSA onto the original OCP …
Post-Operative Carbonate-Apatite Formation In Peo/Pbt Copolymers (Polyactive®), A. M. Radder, J. E. Davies, R. N. S. Sodhi, S. A. T. Van Der Meer, J. G. C. Wolke, C. A. Van Blitterswijk
Post-Operative Carbonate-Apatite Formation In Peo/Pbt Copolymers (Polyactive®), A. M. Radder, J. E. Davies, R. N. S. Sodhi, S. A. T. Van Der Meer, J. G. C. Wolke, C. A. Van Blitterswijk
Cells and Materials
Previous studies have stressed the importance of calcification of the polyethylene oxide (PEO) I polybutylene terephthalate (PBT) copolymer surface by establishing a direct relation with the occurrence of bone-bonding. Here, we characterized the morphology as well as the composition of this post-operative reaction product in PEOIPBT copolymers. X-ray · photoelectron spectroscopical results demonstrated the ability of PEOIPBT copolymers to rapidly adsorb calcium ions from fluids. After subcutaneous implantation in rats, it was shown that polymer calcification comprised plate-shaped crystals, whereas an electron-dense layer was frequently encountered at the interface. Cells with a characteristic morphology were directly apposed to abundantly calcified …
Bone Absorptiometry In Metabolic Bone Disease: Baseline Values And Long-Term Treatment With Calcitriol (Post-Menopausal Osteoporosis Versus Osteomalacia), A. Caniggia, B. Frediani
Bone Absorptiometry In Metabolic Bone Disease: Baseline Values And Long-Term Treatment With Calcitriol (Post-Menopausal Osteoporosis Versus Osteomalacia), A. Caniggia, B. Frediani
Cells and Materials
Total body bone absorptiometry reveals low mineral density in both postmenopausal osteoporosis and osteomalacia patients. The method was used to investigate the effect of calcitriol administration on patients suffering from one of these conditions. In osteomalacia, the administration of calcitriol resulted in a dramatic improvement in bone mineral density (sometimes up to 50% in 12 months), indicating the rapid mineralization of previously uncalcified bone tissue as a result of the normalization of the Ca x P product. In osteoporosis a similar treatment was seen to halt the progressive decrease in bone mineral levels and sometimes resulted in minor increases in …
Resorption Of Natural Calcium Carbonate By Avian Osteoclasts In Vitro, G. Guillemin, S. J. Hunter, C. V. Gay
Resorption Of Natural Calcium Carbonate By Avian Osteoclasts In Vitro, G. Guillemin, S. J. Hunter, C. V. Gay
Cells and Materials
Osteoclasts isolated from the endosteum of 2.5 to 3-week chick tibia were cultured on glass coverslips or natural CaC03 (Tridacna) wafers for 2 and 4 days. The cells were exposed to the pH-dependent dye, acridine orange, and fluorescence was measured by a light microscope photometer. Fluorescence intensity values were higher in cells adherent to Tridacna wafers than in those incubated on glass after 2 and 4 days of culture (three and two-fold, respectively). Moreover, osteoclasts on Tridacna wafers were more flattened and were found to produce resorption pits. Acid production by osteoclasts cultured on Tridacna wafers was stimulated …
Cell Adhesion To Crystal Surfaces: A Model For Initial Stages In The Attachment Of Cells To Solid Substrates, D. Hanein, B. Geiger, L. Addadi
Cell Adhesion To Crystal Surfaces: A Model For Initial Stages In The Attachment Of Cells To Solid Substrates, D. Hanein, B. Geiger, L. Addadi
Cells and Materials
This study addresses the mechanism of the chirally-restricted, ROD-independent adhesion of A6 epithelial cells to the {011} faces of calcium {R,R)-tartrate tetrahydrate crystals. The extensive and rapid adhesion of the cells to these surfaces, in the presence or absence of serum proteins, is distinctly different from the extracellular matrix-mediated adhesion to conventional tissue culture surfaces or to the {101} faces of the same crystals. The differences are manifested by insensitivity to ATP depletion, to disruption of microfilaments and microtubules and even to formaldehyde fixation of the cells. Furthermore, trypsin pretreatment does not affect cell attachment to the {011} faces, nor …
Room Temperature Aging Of Pd-Cu-Ga Dental Alloys, William A. Brantley, Zhuo Cai, Qiang Wu, Alan B. Carr, John C. Mitchell
Room Temperature Aging Of Pd-Cu-Ga Dental Alloys, William A. Brantley, Zhuo Cai, Qiang Wu, Alan B. Carr, John C. Mitchell
Cells and Materials
Specimens of three Pd-Cu-Ga dental alloys cast five years ago and subsequently stored at room temperature were reexamined and observed to have lower amounts of the eutectic constituents in the near-surface region than originally present, along with other microstructural changes. This previously unreported room temperature aging behavior of these alloys is attributed to the presence of high-diffusivity paths in the non-equilibrium ascast eutectic structures and to the essential role of the surface for the vacancy diffusion mechanism. These results may have important clinical significance for the ill vivo corrosion resistance and long-term biocompatibility of the Pd-Cu-Ga alloys.
Adsorption Of Proteins At Solid-Liquid Interfaces, Willem Norde
Adsorption Of Proteins At Solid-Liquid Interfaces, Willem Norde
Cells and Materials
This paper concisely reviews the general principles underlying protein adsorption from aqueous solution onto a solid surface. The discussion includes the various stages of the adsorption process, i. e., transport of the protein molecules towards the surface, the absorbed amount under equilibrium conditions, desorption and re-adsorption. Among the interactions that determine the overall protein adsorption process (1 ) redistribution of charged groups in the interfacial layer, (2) changes in the hydration of the sorbent and the protein surface, and (3) structural rearrangements in the protein molecule play major roles. Special attention is given to the relation between the structural stability …
Hip Prosthesis And Bone Mineral Absorptiometry: Effect Of Short-Term Administration Of Calcitriol, Mario Caniggia, Bruno Frediani, Leonardo Osti
Hip Prosthesis And Bone Mineral Absorptiometry: Effect Of Short-Term Administration Of Calcitriol, Mario Caniggia, Bruno Frediani, Leonardo Osti
Cells and Materials
In this double blind study, seven out of fourteen patients who had received a cementless hydroxyapatitecovered hip prosthesis were given calcitriol (1 p.g daily), and seven an inert placebo for a period of four months. At the end of this period, total body bone densitometry (TBBD) and absorptiometric measurements in the regions of interest (ROIs) close to the prosthesis and in the opposite femur revealed that the TBBD values had decreased in the patients receiving placebo and increased in those receiving calcitriol. Furthermore, bone mineral density in the ROis had increased on the non-operated side of the calcitriol-treated patients (although …
Backscattered Electron Imaging To Enhance Microstructural Contrast In Poly(Methyl Methacrylate) Bone Cement Fracture Analysis, L. D. T. Topoleski, P. Rutledge, X. Lu
Backscattered Electron Imaging To Enhance Microstructural Contrast In Poly(Methyl Methacrylate) Bone Cement Fracture Analysis, L. D. T. Topoleski, P. Rutledge, X. Lu
Cells and Materials
Poly(methyl methacrylate) (PMMA) bone cement is used as a grout to secure joint replacement prostheses into bone. It has a distinct microstructure made up of: prepolymerized beads, an interbead matrix polymer, a radiopacifier {barium sulphate (BaS04) or zirconium dioxide (Zr02)}, and pores or voids; the radiopacifier is found only in the interbead matrix of the cured bone cement. The mechanism of slow or fatigue crack growth appears to be initial micro-cracking through the interbead matrix, followed by coalescence of the microcracks to form a continuous crack. Thus, distinguishing the interbead matrix from the pre-polymerized beads is important for investigating fatigue …
Reliability Of Human Fresh And Frozen Gingiva Explant Culture In Assessing Dental Materials Cytocompatibility, R. Warocquier-Clerout, G. C. Hachom-Nitcheu, M. F. Sigot-Luizard
Reliability Of Human Fresh And Frozen Gingiva Explant Culture In Assessing Dental Materials Cytocompatibility, R. Warocquier-Clerout, G. C. Hachom-Nitcheu, M. F. Sigot-Luizard
Cells and Materials
An explant culture of human gingival epithelium has been set up in order to provide a valuable test for evaluating the cytocompatibility of dental material s. In an attempt to supply a bank of gingiva ex plants, frozen and freshly excised specimens were cultured in parallel. Optical and scanning electron microscopy showed an early release of cuboidal cells forming a dense layer around the explants. Afterwards, cultures evolved differently . Spread cells grew and migrated more rap idly in fresh than in frozen explant cultures but their adhesion to substratum increased earlier in frozen ones. Epithelial phenotype of cells had …
Biodegradation Of Synthetic Biphasic Calcium Phosphate And Biological Calcified Substratum By Cells Of Hemopoietic Origin, A. Soueidan, O. I. Gan, J. M. Bouler, F. Gouin, G. Daculsi
Biodegradation Of Synthetic Biphasic Calcium Phosphate And Biological Calcified Substratum By Cells Of Hemopoietic Origin, A. Soueidan, O. I. Gan, J. M. Bouler, F. Gouin, G. Daculsi
Cells and Materials
Different types of osteoclastic cells (authentic osteoclasit from human giant cell tumor and bone marrow of newborn rats; newly-formed osteoclasts from adult rat bone marrow), giant multinucleated cells and macrophages were studied for their effect on synthetic and natural mineralized substrata. Biphasic calcium phosphate ceramic consisted of hydroxyapatite and beta tricalcium phosphate was chosen for in vitro experiments, and dentine served as a positive control for cell resorbing activity . Our results show the limited capacity of authentic and newly-formed osteoclasts to resorb synthetic ceramic as compared to that of natural substrata. In vitro cell-mediated biodegradation included also modifications of …
A Morphological Assessment Of Bovine Chondrocytes Cultured On Poly(Ethyl Methacrylate)/Tetrahydrofurfuryl Methacrylate, R. M. Sawtell, M. V. Kayser, S. Downes
A Morphological Assessment Of Bovine Chondrocytes Cultured On Poly(Ethyl Methacrylate)/Tetrahydrofurfuryl Methacrylate, R. M. Sawtell, M. V. Kayser, S. Downes
Cells and Materials
A heterocyclic methacrylate polymer system, PEMA/THFMA, has shown potential as a biomaterial for cartilage repair in a rabbit model and has properties making it suitable for use clinically. The ability of the polymer system, PEMA/THFMA, and a related system, PEMA/HEMA, to support chondrocytes in vitro was assessed by scanning electron microscopy. Chondrocytes adhered to the surface of the PEMA/THFMA by day one, having a rounded morphology and clustered appearance opposed to the Thermanox® control, where the cells had spread out and become fibroblastic in appearance. The chondrocytes divided rapidly on the PEMA/ THFMA system by day three and had completely …
Synthetic Hydrogel As An Artificial Vitreous Body. A One-Year Animal Study Of Its Effects On The Retina, T. V. Chirila, I. J. Constable, Y. Hong, S. Vijayasekaran, M. F. Humphrey, P. D. Dalton, S. G. Tahija, M. A. L. Maley, M. J. H. Cuypers, C. Sharp, S. R. Moore, M. J. Davies
Synthetic Hydrogel As An Artificial Vitreous Body. A One-Year Animal Study Of Its Effects On The Retina, T. V. Chirila, I. J. Constable, Y. Hong, S. Vijayasekaran, M. F. Humphrey, P. D. Dalton, S. G. Tahija, M. A. L. Maley, M. J. H. Cuypers, C. Sharp, S. R. Moore, M. J. Davies
Cells and Materials
A hydrogel with a high water content was assessed in vitro and in vivo as a possible vitreous substitute. From a large series of polymers produced by the aqueous polymerization of methyl acrylamidoglycolate methyl ether (MAGME), a gel synthesized in 80% water was selected for an animal study. The gel was injected intravitreally into rabbit eyes and followed clinically by ophthalmoscopy, tonometry, and fundus photography. The gel was clinically well tolerated, but after 6 months ophthalmoscopy revealed progressive pallor of the optic nerve head. The eyes were enucleated one year after injection of polymer. Histopathological examination by light microscopy of …
Co-Culture Of Endothelial Cells And Smooth Muscle Cells In A Flow Environment: An Improved Culture Model Of The Vascular Wall?, Thierry Ziegler, Keith A. Robinson, R. Wayne Alexander, Robert M. Nerem
Co-Culture Of Endothelial Cells And Smooth Muscle Cells In A Flow Environment: An Improved Culture Model Of The Vascular Wall?, Thierry Ziegler, Keith A. Robinson, R. Wayne Alexander, Robert M. Nerem
Cells and Materials
Numerous studies have demonstrated that the neighboring smooth muscle cells (SMC) influence the morphology, cytoskeleton and growth of co-cultured endothelial cells (EC). Also, flow-induced laminar shear stress has been shown to induce cell elongation, F-actin reorganization and growth inhibition in cultured EC. We investigated the effect of neighboring SMC and collagen matrix on the response of EC to shear stress. The co-culture system was made by growing porcine aortic SMC in a gel of collagen type I and then seeding porcine aortic EC (P AEC) on the top surface. Then the co-culture was exposed to steady, laminar shear stress of …
Natural History Of Bone Response To Hydroxyapatite-Coated Hip Prostheses Implanted In Humans, P. Frayssinet, D. Hardy, J. S. Hanker, B. L. Giammara
Natural History Of Bone Response To Hydroxyapatite-Coated Hip Prostheses Implanted In Humans, P. Frayssinet, D. Hardy, J. S. Hanker, B. L. Giammara
Cells and Materials
A series of 15 autopsied femurs containing hydroxyapatite- coated (HA-coated) prostheses was analysed histologically. Their implantation time ranged from 5 days up to 3 years. The coating thickness of some prostheses and the percentage of the coating in contact with bone at different levels were evaluated using an image analysis device. After the newly formed bone tissue had became mature, several bone morphotypes were identified at the coating contact. From the proximal to the distal part of the prosthesis, bone morphotype was denser and the percentage of the coating surface in contact with bone increased. Several stages in the prosthesis …
Biomaterials Associated Infections: The Scale Of The Problem, David J. Stickler, Robert J. C. Mclean
Biomaterials Associated Infections: The Scale Of The Problem, David J. Stickler, Robert J. C. Mclean
Cells and Materials
The biomaterials used in the manufacture of implanted prosthetic devices profoundly impair the host's ability to opsonise and phagocytose invading microbes. As a result, while these devices generally provide effective relief from painful, crippling and life-threatening disorders, they can also induce vulnerability to infection in the recipients. The surfaces of the implants are susceptible to colonisation by microbial biofilms. The cells in the biofilm.s are further protected against opsonophagocytosis and are also resistant to antibacterials. Device associated infections thus tend to be refractile to antibiotic therapy and in many cases the device has to be removed before the infection will …
Some Observations On The Structure Of Encrusting Biofilms Of Proteus Mirabilis On Urethral Catheters, C. Winters, D. J. Stickler, N. S. Howe, T. J. Williams, N. Wilkinson, C. J. Buckley
Some Observations On The Structure Of Encrusting Biofilms Of Proteus Mirabilis On Urethral Catheters, C. Winters, D. J. Stickler, N. S. Howe, T. J. Williams, N. Wilkinson, C. J. Buckley
Cells and Materials
A physical model of the bladder has been used to study the process of urinary catheter encrustation. Techniques have been devised for the preparation of sections through these encrustations in situ on the luminal surfaces of catheters and for mapping the distribution of calcium and magnesium in the biofilms. Transmission electron microscopy on these sections showed struvite-like crystals lying in the matrix and in direct contact with the catheter surface. Calcium phosphate ("bioapatite") was distributed throughout the film and many of these amorphous particles appear to have cells at their cores. Freeze-substituted sections of biofilms also showed electron dense materials …
Some Biological And Physical Properties Of Laser Deposited Hydroxyapatite Based Films, Tatjana Dostalova, Lucie Himmlova, Miroslav Jelinek, Jirina Bartova
Some Biological And Physical Properties Of Laser Deposited Hydroxyapatite Based Films, Tatjana Dostalova, Lucie Himmlova, Miroslav Jelinek, Jirina Bartova
Cells and Materials
The preliminary results of biological and physical tests of hydroxyapatite thin films deposited on dental implants by a new technology with the KrF excimer laser ablation method were evaluated. Biological and physical properties were studied and analyzed by the lymphocyte proliferation test and scanning electron microscopy (SEM), X-ray analysis, Rutherford backscattering analysis (RBS) and particle induced X-ray emission (PIXE) methods. Ten bioceramic films from 45 samples had very good physical and biological properties. Creation of hydroxyapatite thin films with laser ablation can have a positive effect on adhesion of the film and protection for corrosion.
Biphasic Sodium Fluoride Effects On Bone And Bone Mineral: A Review, P. -T. Cheng, S. M. Bader, M. D. Grynpas
Biphasic Sodium Fluoride Effects On Bone And Bone Mineral: A Review, P. -T. Cheng, S. M. Bader, M. D. Grynpas
Cells and Materials
This paper reviews the clinical and experimental findings on the effects of sodium fluoride (NaF) on human and animal bone. NaF has been shown to cause a significant increase in axial skeletal bone mass. However, there is concern that the new bone may not provide the desired increase in bone strength. Yet, NaF remains the most commonly used agent capable of stimulating bone formation in most patients (30% non-responders). But whether NaF reduces vertebral fracture rate (VFR) remains controversial. For a given treatment duration, the effect of F on bone quality appears to depend on dose: there is a marked …
Optical Bone Chambers As Tools For Studying The Bone-Implant Interface: A Review, Howard Winet
Optical Bone Chambers As Tools For Studying The Bone-Implant Interface: A Review, Howard Winet
Cells and Materials
Bone chamber intravital microscopy combines the control volume of in vitro models and the chemical complexity of in vivo models to optimize the study of bone physiology in situ. As an optical tool it provides a window to dynamic events at the tissue level of magnification. In particular, it allows measures of microvascular events: (1) in space by magnifying local perfused vasculature and microcirculation at any instant, and (2) in time by providing the same volume of tissue for weekly viewing of an evolving process such as bone healing. This quartz-bearing titanium screw has revealed a consistent order for …