Open Access. Powered by Scholars. Published by Universities.®

Engineering Commons

Open Access. Powered by Scholars. Published by Universities.®

Theses/Dissertations

Chemical engineering

Retrospective Theses and Dissertations

Plant Sciences

Publication Year

Articles 1 - 3 of 3

Full-Text Articles in Engineering

Carbon-13 Metabolic Flux Analysis Of Soybean Central Carbon Metabolism: Response To Temperature And Genotype Effects , Vidya Vaancheeswaran Iyer Jan 2006

Carbon-13 Metabolic Flux Analysis Of Soybean Central Carbon Metabolism: Response To Temperature And Genotype Effects , Vidya Vaancheeswaran Iyer

Retrospective Theses and Dissertations

Soybeans are a major source of high quality protein, edible oil, food products, and also several industrial non food applications such as paper coating, textiles and plastics. It is difficult to develop soybeans with high protein and high oil, however, due to the inverse relationship between these two components. Consequently, increasing seed protein concentration without adversely affecting the yield and oil content has been difficult to achieve. It has been demonstrated that the temperature and genotype of soybeans influence the protein or lipid composition considerably. However, the interactions between various pathways responsible for the changes in the composition are unknown ...


Flux Analysis In Central Carbon Metabolism In Plants: 13c Nmr Experiments And Analysis , Ganesh Sriram Jan 2004

Flux Analysis In Central Carbon Metabolism In Plants: 13c Nmr Experiments And Analysis , Ganesh Sriram

Retrospective Theses and Dissertations

Metabolic flux analysis is crucial in metabolic engineering. This research concentrated on improvements in 13C labeling-based flux analysis, a powerful flux quantification method, particularly oriented toward application to plants. Furthermore, systemic 13C flux analyses were performed on two model plant systems: Glycine max (soybean) embryos, and Catharanthus roseus hairy roots.;The concepts 'bond integrity', 'bondomer' and the algorithm 'Boolean function mapping' were introduced, to facilitate efficient flux evaluation from carbon bond labeling experiments, and easier flux identifiability analysis.;13C labeling experiments were performed on developing soybean (Glycine max) embryos and C. roseus hairy roots. A computer program, NMR2Flux, was developed ...


Tnt Phytotransformation In Native And Genetically Modified Species: Significance Of Aromatic Hydroxylamines In The Metabolic Pathway , Murali Subramanian Jan 2004

Tnt Phytotransformation In Native And Genetically Modified Species: Significance Of Aromatic Hydroxylamines In The Metabolic Pathway , Murali Subramanian

Retrospective Theses and Dissertations

The presence and role of hydroxylamines in TNT transformation were ascertained in two axenic (microbe-free) hydroponic plant systems through high TNT feeding experiments and the use of different analytical detection schemes. Hydroxylamines were formed within 10 hours of TNT addition, and usually disappeared within 20 hours, displaying a high turnover rate due to their instability and high reactivity. Hydroxylamines were shown to add biomolecules to their functional group and thereby directly form conjugates. The 4-isomer of the hydroxylamine was detected in very high levels (up to 30% of initial TNT added) in a transgenic tobacco system that had a bacterial ...