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Full-Text Articles in Engineering

Engineering Polyanhydride Microspheres For The Stabilization And Controlled Release Of Proteins , Amy Sywassink Determan Jan 2006

Engineering Polyanhydride Microspheres For The Stabilization And Controlled Release Of Proteins , Amy Sywassink Determan

Retrospective Theses and Dissertations

This work investigates the use of polyanhydride microspheres as drug delivery carriers for therapeutic proteins. The polymers investigated are poly(sebacic anhydride) and opolymers of poly[1,6-bis(p-carboxyphenoxy)hexane] (poly(CPH)) and poly(SA), 20:80 (CPH:SA), 50:50 (CPH:SA), and 80:20 (CPH:SA). The model protein bovine serum albumin (BSA) was encapsulated in poly(SA), 20:80 (CPH:SA), 50:50 (CPH:SA), and 80:20 (CPH:SA) microspheres to determine the feasibility of using polyanhydrides as protein carriers. Poly(SA) and 20:80 (CPH:SA) were found to stabilize the encapsulated BSA and were ...


Multicomponent Drying Of Semicrystalline Polymers , Sim-Siong Wong Jan 2006

Multicomponent Drying Of Semicrystalline Polymers , Sim-Siong Wong

Retrospective Theses and Dissertations

The focus of this research is to gain a better understanding of multicomponent drying behavior of semicrystalline polymers. A combination of experimental and theoretical approaches was used in this work. A mathematical model involving free-volume theory and solvent-induced crystallization kinetics as the backbone was developed to examine the complex relationship between polymers and solvents during drying. External conditions such as temperature, external mass transfer rate and film shrinkage were considered. A poly(vinyl alcohol)/water/methanol test system was chosen. The parametric studies of the model show that it can be adapted for wide range of initial and operating conditions ...


Computational Fluid Dynamics Simulation Of Fluidized Bed Polymerization Reactors , Rong Fan Jan 2006

Computational Fluid Dynamics Simulation Of Fluidized Bed Polymerization Reactors , Rong Fan

Retrospective Theses and Dissertations

In this research, a CFD algorithm for simulation of fluidized bed polymerization reactors is described. In order to properly model the evolution of a polydisperse solid phase, population balance equation (PBE) must be solved along with other transport equations. A novel approach---DQMOM is applied to polydisperse fluidized bed to simulate particle aggregation and breakage in the reactors. Two different aggregation and breakage kernels are tested and the performance of the DQMOM approximation with different numbers of nodes are compared. Results show that the approach is very effective in modeling solid segregation and elutriation and in tracking the evolution of the ...


Computational Methods In The Study Of Carbohydrates And Carbohydrate-Active Enzymes , Anthony David Hill Jan 2006

Computational Methods In The Study Of Carbohydrates And Carbohydrate-Active Enzymes , Anthony David Hill

Retrospective Theses and Dissertations

Carbohydrates are important molecules in energy storage and cellular recognition. New methods are used and discussed to capture important structural details of created by the protein/carbohydrate interactions. In particular, the thermodynamics of carbohydrate binding are explored, and a new model to accurately calculate the free energy of binding is developed. Additionally, a new method of describing the pucker of carbohydrate ring with three coordinates is discussed. These methods are then applied to Family 1 Glycoside Hydrolases.


Carbon-13 Metabolic Flux Analysis Of Soybean Central Carbon Metabolism: Response To Temperature And Genotype Effects , Vidya Vaancheeswaran Iyer Jan 2006

Carbon-13 Metabolic Flux Analysis Of Soybean Central Carbon Metabolism: Response To Temperature And Genotype Effects , Vidya Vaancheeswaran Iyer

Retrospective Theses and Dissertations

Soybeans are a major source of high quality protein, edible oil, food products, and also several industrial non food applications such as paper coating, textiles and plastics. It is difficult to develop soybeans with high protein and high oil, however, due to the inverse relationship between these two components. Consequently, increasing seed protein concentration without adversely affecting the yield and oil content has been difficult to achieve. It has been demonstrated that the temperature and genotype of soybeans influence the protein or lipid composition considerably. However, the interactions between various pathways responsible for the changes in the composition are unknown ...


A Comprehensive Dynamic Model For High-Pressure Tubular Low-Density Polyethylene (Ldpe) Reactors , Mothivel B. Mummudi Boopathy Jan 2006

A Comprehensive Dynamic Model For High-Pressure Tubular Low-Density Polyethylene (Ldpe) Reactors , Mothivel B. Mummudi Boopathy

Retrospective Theses and Dissertations

Low-density polyethylene (LDPE) is a versatile polymer which is widely used both in domestic and industrial applications. About 50% of commercial LDPE production around the world is carried out by free-radical polymerization initiated by organic peroxides in high-pressure tubular reactors. The free-radical polymerization is highly sensitive to even small changes in process conditions. As a result, the extreme pressures and temperatures in the high-pressure tubular LDPE reactor (HPTLR) present operational challenges to the plant engineer. A reliable model of the HPTLR will prove to be a valuable tool for the plant engineer in making decisions about reactor operations;In this ...


Directed Growth And Selective Differentiation Of Neural Progenitor Cells Using A Synergistic Combination Of Topographical And Soluble Cues , Jennifer Behr Recknor Jan 2006

Directed Growth And Selective Differentiation Of Neural Progenitor Cells Using A Synergistic Combination Of Topographical And Soluble Cues , Jennifer Behr Recknor

Retrospective Theses and Dissertations

The purpose of this study is to develop strategies to facilitate nerve regeneration using a synergistic combination of guidance cues. We are investigating the cellular mechanisms of development using adult rat hippocampal progenitor cells (AHPCs) and have demonstrated that manipulating a combination of physical, chemical and biological cues can lead to oriented astrocyte and neural progenitor cell outgrowth and can influence progenitor cell differentiation. To provide physical guidance, micropatterned polystyrene (PS) substrates were fabricated and chemically modified with laminin. Astrocytes or AHPCs cultured on these substrates align along the grooves of the patterned surface extending highly elongated processes. To further ...


Recovery Of Monosaccharides Via Catalytic Hydrolysis , Jason Alan Bootsma Jan 2006

Recovery Of Monosaccharides Via Catalytic Hydrolysis , Jason Alan Bootsma

Retrospective Theses and Dissertations

This research has provided a fundamental analysis of the use of novel separation and catalyst techniques for saccharification of lignocellulosic tissues. Separation techniques focused on providing a feedstock that was more amenable to hydrolysis. Catalyst development focused on solid acid materials that would provide processing advantages, cost reductions, and waste reduction. A mechanical separation technique was used on corn stover to separate the parent material into two streams with improved processing characteristics. The desire was to produce a material that was easier to hydrolyze in addition to producing a material with enhanced fiber character. The separation technique was effective; however ...


Recovery And Purification Of Recombinant Protein From Transgenic Corn By Aqueous Two-Phase Partitioning , Zhengrong Gu Jan 2006

Recovery And Purification Of Recombinant Protein From Transgenic Corn By Aqueous Two-Phase Partitioning , Zhengrong Gu

Retrospective Theses and Dissertations

Aqueous two-phase (ATP) partitioning was paired in a variety of ways to use of transgenic corn for protein expression. Systems were developed to selectively partition corn proteins from classes of potential recombinant proteins as well as the specific case of dog gastric lipase. More fundamentally, ATP partitioning was used to expand 2D gel electrophoretic characterization of protein size and pI to the third dimension of hydrophobicity with the usefulness of the method illustrated for corn extracts;ATP partitioning in polyethylene glycol (PEG)-salt systems separates proteins primarily according to their surface hydrophobicity differences. The average hydrophobicity of pH 7 extracts ...


Vibration-Induced Mobilization Of Trapped Non-Aqueous Phase Liquids In Porous Media , Wenqing Li Jan 2006

Vibration-Induced Mobilization Of Trapped Non-Aqueous Phase Liquids In Porous Media , Wenqing Li

Retrospective Theses and Dissertations

Acoustic wave stimulation, such as vibration-induced mobilization, is a promising enhancement approach to remove trapped NAPLs (Non-Aqueous Phase Liquids) usually encountered in multiphase flows through porous media, especially the remediation of underground water contamination and incomplete petroleum recovery from oil reservoirs, with advantages of high efficiency, low cost and environmental safety relative to traditional mobilization methods;According to a simple hypothesized capillary-physics mechanism, specific predictions can be deduced that vibration will be the most effective in mobilizing trapped non-aqueous phase liquids with a comparative higher acceleration amplitude and lower vibration frequency;Quasi-two-dimensional glass micro-model experiments were carried out and it ...


Synthesis And Characterization Of Stimuli Responsive Block Copolymers, Self-Assembly Behavior And Applications , Michael Duane Determan Jan 2006

Synthesis And Characterization Of Stimuli Responsive Block Copolymers, Self-Assembly Behavior And Applications , Michael Duane Determan

Retrospective Theses and Dissertations

Water-soluble stimuli responsive block copolymers are a rapidly emerging class of materials with great potential in biomedical and technological applications. In this work a novel class of pentablock copolymers are synthesized via atom transfer radical polymerization techniques and their stimuli-responsive self-assembly properties are characterized. Aqueous solutions of these materials are observed to form micelles and hydrogels in response to changes in both temperature and pH. Cryogenic transmission electron microscopy (cryo-TEM) and small angle neutron and X-ray scattering (SANS and SAXS) techniques are used to investigate the nanoscale structures formed by these pentablock copolymers in solution. The gel structure and mechanical ...


Synthesis And Characterization Of Nanostructured Aluminas , Weihua Deng Jan 2005

Synthesis And Characterization Of Nanostructured Aluminas , Weihua Deng

Retrospective Theses and Dissertations

In the work, mesoporous aluminas were produced by nonionic supramolecular templating and characterized to determine the stability of their physical structure during extended calcination at elevated temperatures. The effect of synthesis temperature on the physical structure of these materials and the resulting thermal evolution from synthesized aluminum hydroxide to transitional alumina was studied using thermogravimetric analysis. A high resolution multiple quantum magic angle spinning NMR spectroscopy method was used to determine quantitatively the evolution of the aluminum coordination during the thermal processing. The mesoporous alumina exhibited high stability upon prolonged heating, which is essential for their future applications in catalytic ...


Mechanistic Insight Into The Production Of Ethylene Glycol And Propylene Glycol From Biorenewable Resources , Daniel George Lahr Jan 2005

Mechanistic Insight Into The Production Of Ethylene Glycol And Propylene Glycol From Biorenewable Resources , Daniel George Lahr

Retrospective Theses and Dissertations

The production of ethylene glycol and propylene glycol from biorenewable resources, i.e. sorbitol, has been parametrically examined numerous times. However, the current selectivity is too low to be economically feasible. In order to develop improved catalyst systems, a better understanding of the reaction mechanism is required. Sorbitol, due to its many functional groups is quite complex. Thus, in order to begin understanding of this system, analysis of the products is required. The study of the degradation of the product glycols and reaction of glycerol under hydrogenolysis conditions gives foundational insight. The effects of pH, competitive adsorption, and product degradation ...


Force Computations In Automated Docking , Chandrika Mulakala Jan 2005

Force Computations In Automated Docking , Chandrika Mulakala

Retrospective Theses and Dissertations

Automated docking refers to the problem of computing the optimal complementary fit of two molecules---a macromolecular receptor such as DNA or protein and a small molecule of interest (ligand). AutoDock is a docking software that computes the receptor-ligand binding energy to rank docked ligands. In this work, AutoDock's grid-based method for energy evaluation was exploited to complement the binding energies with computed forces on docked ligand atoms. These forces and energies helped to provide insights into enzyme-substrate interaction mechanisms of three different enzymes--- Hypocrea jecorina Cel7A, a cellobiohydrolase, Fusarium oxysporum Cel7B, an endoglucanase, and Saccharomyces cerevisiae alpha-1,2-mannosidase. Cel7A ...


Continuous-Time Block-Oriented Nonlinear Modeling With Complex Input Noise Structure , Dongmei Zhai Jan 2005

Continuous-Time Block-Oriented Nonlinear Modeling With Complex Input Noise Structure , Dongmei Zhai

Retrospective Theses and Dissertations

The continuous-time closed-form algorithms to sinusoidal input changes are proposed and presented for single-input, single-output (SISO) Hammerstein and Wiener systems with the first-order, second-order, and second-order plus lead dynamics. By simulation on theoretical Hammerstein and Wiener systems, the predicted responses agree exactly with the true process values. They depend on only the most recent input change. The algorithms to SISO Hammerstein and Wiener systems can be conveniently extended to the multiple-input, multiple-output (MIMO) systems as shown by the two-input, two-output examples and demonstrated by the simulated seven-input, five-output continuous stirred tank reactor (CSTR). The predictions and the simulated theoretical responses ...


Design Of Catalytic Domains Within Organosulfonic Acid-Functionalized Mesoporous Silica , Isa Kipkoech Mbaraka Jan 2005

Design Of Catalytic Domains Within Organosulfonic Acid-Functionalized Mesoporous Silica , Isa Kipkoech Mbaraka

Retrospective Theses and Dissertations

Organic-inorganic hybrid mesoporous silicas were synthesized via nonionic supramolecular assembly using the co-condensation synthesis technique with the intention of understanding the catalytic domain as well as developing strategies to control the catalytic functionalities at the molecular level. Esterification of free fatty acids in natural oils and fats was used to probe the catalytic activity of the synthesized acid-modified mesoporous catalysts. Application of solid acid catalysts in such reaction systems can possibly simplify the manufacturing process, reducing production costs, as well as eliminate environmental hazards posed by toxic wastes generated in homogeneous catalyzed reactions;The catalytic performance of the functionalized mesoporous ...


The Role Of Reduction In The Deactivation Of Potassium-Promoted Iron Oxide Dehydrogenation Catalysts , Sipho C. Ndlela Jan 2004

The Role Of Reduction In The Deactivation Of Potassium-Promoted Iron Oxide Dehydrogenation Catalysts , Sipho C. Ndlela

Retrospective Theses and Dissertations

One of the largest commercial application for potassium promoted iron oxide catalyst (K-Fe2O3) in petrochemical industry is in the dehydrogenation of ethylbenzene (EB) to styrene (ST). It is generally accepted that the active sites on the K-Fe2O3 catalyst is potassium ferrite (KFeO2), which resides on the surface of a bulk magnetite phase and potassium polyferrite (K2Fe22O 34). This dehydrogenation reaction is typically performed in excess steam and the catalyst is known to experience short-term deactivation when the steam-to-hydrocarbon molar ratio (S/EB) is lowered. While possible causes for the deactivation phenomena are coking or reduction of the reactive site, the ...


Flux Analysis In Central Carbon Metabolism In Plants: 13c Nmr Experiments And Analysis , Ganesh Sriram Jan 2004

Flux Analysis In Central Carbon Metabolism In Plants: 13c Nmr Experiments And Analysis , Ganesh Sriram

Retrospective Theses and Dissertations

Metabolic flux analysis is crucial in metabolic engineering. This research concentrated on improvements in 13C labeling-based flux analysis, a powerful flux quantification method, particularly oriented toward application to plants. Furthermore, systemic 13C flux analyses were performed on two model plant systems: Glycine max (soybean) embryos, and Catharanthus roseus hairy roots.;The concepts 'bond integrity', 'bondomer' and the algorithm 'Boolean function mapping' were introduced, to facilitate efficient flux evaluation from carbon bond labeling experiments, and easier flux identifiability analysis.;13C labeling experiments were performed on developing soybean (Glycine max) embryos and C. roseus hairy roots. A computer program, NMR2Flux, was developed ...


Engineering Bioerodible Polymers With Tailored Micro/Nanostructure For Vaccine Delivery , Matthew J. Kipper Jan 2004

Engineering Bioerodible Polymers With Tailored Micro/Nanostructure For Vaccine Delivery , Matthew J. Kipper

Retrospective Theses and Dissertations

This work describes the investigation of bioerodible polyanhydrides as controlled drug delivery vehicles. The polymers studied are based on the 1,6-bis(p-carboxyphenoxy)hexane (CPH) and sebacic acid (SA) monomers. These two materials erode at vastly different rates and can be combined in random copolymers or blends to achieve tailored erosion kinetics. The hydrophobic nature of these materials offers the potential to stabilize proteins, and their mutual incompatibility and semicrystallinity provide an interesting phase behavior, which can be exploited to aid in tailoring the release kinetics. Theoretical and experimental description of the microstructure of polyanhydride copolymers reveals the details of ...


Combinatorial Synthesis And Screening Of Fuel Cell Catalysts , Shrisudersan Jayaraman Jan 2004

Combinatorial Synthesis And Screening Of Fuel Cell Catalysts , Shrisudersan Jayaraman

Retrospective Theses and Dissertations

Polymer electrolyte membrane fuel cells (PEMFCs) are compact power sources that can operate with high efficiencies and low emission of environmentally harmful gases. One of the major barriers impeding the development of PEMFCs as a competitive energy source is the inability of existing anode catalysts to oxidize fuels other than hydrogen at sufficient levels due to catalyst deactivation by carbon monoxide (CO) and other partial oxidation products. The focus of this research is the development and application of combinatorial strategies to construct and interrogate electrooxidation (anode) catalysts pertaining to PEMFCs to discover catalysts with enhanced performance in catalyst deactivating environments ...


Theoretical And Experimental Explorations In Atomic Force Microscopy , Anil Gannepalli Jan 2004

Theoretical And Experimental Explorations In Atomic Force Microscopy , Anil Gannepalli

Retrospective Theses and Dissertations

Nanotechnology is the capability to build by controlling the arrangement of individual atoms and molecules. Such a technology would be founded on the ability to control, manipulate and investigate matter at the atomic scale. The invention of atomic force microscope (AFM) and the advances in micro-cantilever based scanning probe technology have significantly enhanced the experimental capability to probe and modify matter at the nanoscale. However, it is still severely limited in achieving the necessary bandwidth, sensitivity and resolution. To further the advances in this field an in-depth understanding of the nature and effects of the tip-sample interactions is imperative. A ...


Particle Size Prediction In Reactive Precipitation Processes , Jesse Ted Pikturna Jan 2004

Particle Size Prediction In Reactive Precipitation Processes , Jesse Ted Pikturna

Retrospective Theses and Dissertations

The aqueous reductive precipitation of palladium metal has been studied in semibatch stirred tank reactors. Scale-up from the bench scale to the 20 gallon scale has been studied using empirical scale-up rules. Some of the well-known difficulties and benefits associated with empirical methods for scale-up have been observed in this system.;In order to accurately predict particle size distributions (PSDs) in precipitation reactions, local variations in hydrodynamics, degree of supersaturation, and particle size distributions need to be accurately tracked. Discretized Population Balance (DPB) methods can accurately model precipitation process in well-mixed, uniform shear environments, but the implementation of DPB routines ...


Tnt Phytotransformation In Native And Genetically Modified Species: Significance Of Aromatic Hydroxylamines In The Metabolic Pathway , Murali Subramanian Jan 2004

Tnt Phytotransformation In Native And Genetically Modified Species: Significance Of Aromatic Hydroxylamines In The Metabolic Pathway , Murali Subramanian

Retrospective Theses and Dissertations

The presence and role of hydroxylamines in TNT transformation were ascertained in two axenic (microbe-free) hydroponic plant systems through high TNT feeding experiments and the use of different analytical detection schemes. Hydroxylamines were formed within 10 hours of TNT addition, and usually disappeared within 20 hours, displaying a high turnover rate due to their instability and high reactivity. Hydroxylamines were shown to add biomolecules to their functional group and thereby directly form conjugates. The 4-isomer of the hydroxylamine was detected in very high levels (up to 30% of initial TNT added) in a transgenic tobacco system that had a bacterial ...


Computational Fluid Dynamics Simulation Of Precipitation Processes , Liguang Wang Jan 2004

Computational Fluid Dynamics Simulation Of Precipitation Processes , Liguang Wang

Retrospective Theses and Dissertations

First, an experimental and computational investigation of the effects of local fluid shear rate on the aggregation and breakage of ~10 mum latex spheres suspended in an aqueous solution undergoing laminar and turbulent Taylor-Couette flow was carried out, according to the following program. First, computational fluid dynamics (CFD) simulations were performed and the flow field predictions were validated with data from particle image velocimetry experiments. Subsequently, the quadrature method of moments (QMOM) was implemented into the CFD code to obtain predictions for mean particle size that account for the effects of local shear rate on the aggregation and breakage. These ...


Analysis And Design Of A Calcium-Based Sulfur Sorbent For Applications In Integrated Gasification Combined Cycle Energy Systems , David Johann Ludwig Hasler Jan 2003

Analysis And Design Of A Calcium-Based Sulfur Sorbent For Applications In Integrated Gasification Combined Cycle Energy Systems , David Johann Ludwig Hasler

Retrospective Theses and Dissertations

The reactivity of various Ca-based sorbent materials in pelletized form with H2S or CO2 was investigated at high temperatures (750--880°C). An extensive study was conducted to compare the performance of sorbent pellets derived from plaster of Paris and limestone. Multicycle absorption and regeneration tests showed that plaster-based pellets out performed the limestone-based pellets primarily due to a higher surface area and mesoporosity.;The effect of pore-modifiers on the reactivity of limestone with H 2S was investigated by incorporating additives such as cornstarch, graphite and polyvinylalcohol (PVA) in the sorbent. Multicycle sulfidation and regeneration tests of the modified sorbent showed ...


Hybrid Finite-Volume/Transported Pdf Method For The Simulation Of Turbulent Reactive Flows , Venkatramanan Raman Jan 2003

Hybrid Finite-Volume/Transported Pdf Method For The Simulation Of Turbulent Reactive Flows , Venkatramanan Raman

Retrospective Theses and Dissertations

A novel computational scheme is formulated for simulating turbulent reactive flows in complex geometries with detailed chemical kinetics. A Probability Density Function (PDF) based method that handles the scalar transport equation is coupled with an existing Finite Volume (FV) Reynolds-Averaged Navier-Stokes (RANS) flow solver. The PDF formulation leads to closed chemical source terms and facilitates the use of detailed chemical mechanisms without approximations. The particle-based PDF scheme is modified to handle complex geometries and grid structures. Grid-independent particle evolution schemes that scale linearly with the problem size are implemented in the Monte-Carlo PDF solver. A novel algorithm, in situ adaptive ...


Behavior Of Interfacial Micro-Defects In Aluminum And Its Oxide Film , Renchun Huang Jan 2003

Behavior Of Interfacial Micro-Defects In Aluminum And Its Oxide Film , Renchun Huang

Retrospective Theses and Dissertations

In this dissertation, numerical simulation was used to calculate the equilibrium solution composition of cylindrical nanopores in surface oxide films as a step to evaluate their possible roles in the breakdown of protective oxide films. The solution composition of such nanopores is of interest from a corrosion perspective, since they play prominent roles in several mechanisms for the breakdown of passive films leading to pitting corrosion. The chemical composition of the pore solution would determine whether rapid dissolution at the pore could initiate. The effects of nanoscale pore dimensions, solution pH and adsorbing ion borate concentration on the solution composition ...


Biomass Reburning For Control Of Nitrogen Oxides , Jeffrey James Sweterlitsch Jan 2002

Biomass Reburning For Control Of Nitrogen Oxides , Jeffrey James Sweterlitsch

Retrospective Theses and Dissertations

Fuel-Lean Gas Reburning(TM) (FLGR) is a method of controlling NO x emissions produced during coal combustion in utility boilers by injecting natural gas into the boiler downstream of the primary combustion zone. Whereas traditional reburning requires 10%--20% of the total energy input from the reburn fuel followed by over-fire air to complete combustion of fuel fragments, FLGR uses only 5%--10% of the total energy input from the reburn fuel. Because less fuel is used, the overall environment in the boiler remains fuel-lean, with only localized eddies that are fuel-rich, where the NOx reduction takes place. FLGR does ...


Development Of Novel Polymeric Materials For Gene Therapy And Ph-Sensitive Drug Delivery: Modeling, Synthesis, Characterization, And Analysis , Brian Curtis Anderson Jan 2002

Development Of Novel Polymeric Materials For Gene Therapy And Ph-Sensitive Drug Delivery: Modeling, Synthesis, Characterization, And Analysis , Brian Curtis Anderson

Retrospective Theses and Dissertations

The aim of this work was to obtain a fundamental understanding of drug release mechanisms from polymers that undergo thermoreversible gelation and to synthesize new polymers based on these that exhibit both pH and temperature sensitivity. Novel block and random copolymers with cationic character have been developed for drug delivery and gene therapy applications. The development of these materials began with a study of the mechanism of drug release from poly(ethylene oxide) (PEO) and poly(propylene oxide) (PPO) block copolymers. This study revealed the release rates of drugs from water-soluble hydrogels composed of the PEO-PPO-PEO block copolymer PluronicRTM F127 ...


Recovery Of Recombinant Proteins From Transgenic Plants: Strategies In Implementation And Optimization , Todd J. Menkhaus Jan 2002

Recovery Of Recombinant Proteins From Transgenic Plants: Strategies In Implementation And Optimization , Todd J. Menkhaus

Retrospective Theses and Dissertations

Strategies to optimize the recovery of recombinant proteins from plant hosts have been investigated. The use of different crops, different product molecules, and different separation techniques was studied to suggest modes of operation that reduce the overall processing cost for obtaining purified recombinant proteins from plants.;Polyelectrolyte precipitation of beta-glucuronidase (GUS), an acidic model protein, with polyethyleneimine (PEI), a polycatonic polymer, from the extracts of canola, corn germ, soybean, and pea met with varying degrees of success. Precipitation from canola required the least amount of PEI and was capable of providing the highest enrichment. In contrast, soy and pea allowed ...