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Engineering Commons

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Theses/Dissertations

Chemical engineering

Materials Science and Engineering

1999

Articles 1 - 2 of 2

Full-Text Articles in Engineering

Characterizing Growth Rates And Growth Rate Dispersion Of Secondary Nuclei As A Function Of Supersaturation , Christopher Michael Jones Jan 1999

Characterizing Growth Rates And Growth Rate Dispersion Of Secondary Nuclei As A Function Of Supersaturation , Christopher Michael Jones

Retrospective Theses and Dissertations

The growth rates of secondary crystal nuclei determine the number of crystals that will reach a size large enough to contribute to the final product mass in a crystallization process. Contact nuclei were chosen for study because crystals nucleated under conditions of low supersaturation are formed by contact or secondary nucleation. High supersaturation levels are required for primary nucleation to occur. Growth rate dispersion complicates the prediction of growth rates and product size distributions. Having growth rate dispersion in a crystallizing system means that despite the population of crystals having all been nucleated and grown under identical conditions, they grow ...


High Pressure Crystallization Of Purafect® Subtilisin , Ruta Yeshwant Waghmare Jan 1999

High Pressure Crystallization Of Purafect® Subtilisin , Ruta Yeshwant Waghmare

Retrospective Theses and Dissertations

The aim of this work was to study the effect of high pressure on kinetics of subtilisin crystallization and to use high pressure as a means to better understand the mechanism of crystallization;It is known that the effect of hydrostatic pressure on conformation varies with the protein being studied. To determine the effect of high pressure on the conformation of subtilisin, activity assays were used in addition to FTIR spectroscopy. Pressures up to 100 MPa were found to cause no irreversible conformational changes in subtilisin;To study the effect of high pressure on subtilisin crystallization, a factorial experiment was ...