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Theses/Dissertations

Chemical engineering

Biochemistry, Biophysics, and Structural Biology

2002

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Full-Text Articles in Engineering

Recovery Of Recombinant Proteins From Transgenic Plants: Strategies In Implementation And Optimization , Todd J. Menkhaus Jan 2002

Recovery Of Recombinant Proteins From Transgenic Plants: Strategies In Implementation And Optimization , Todd J. Menkhaus

Retrospective Theses and Dissertations

Strategies to optimize the recovery of recombinant proteins from plant hosts have been investigated. The use of different crops, different product molecules, and different separation techniques was studied to suggest modes of operation that reduce the overall processing cost for obtaining purified recombinant proteins from plants.;Polyelectrolyte precipitation of beta-glucuronidase (GUS), an acidic model protein, with polyethyleneimine (PEI), a polycatonic polymer, from the extracts of canola, corn germ, soybean, and pea met with varying degrees of success. Precipitation from canola required the least amount of PEI and was capable of providing the highest enrichment. In contrast, soy and pea allowed ...