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Theses/Dissertations

Chemical engineering

Biochemistry, Biophysics, and Structural Biology

1993

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Full-Text Articles in Engineering

Enhancement Of Polyelectrolyte Precipitation Through The Genetic Fusion Of Charged Polypeptides To Enzymes , Mark Quinn Niederauer Jan 1993

Enhancement Of Polyelectrolyte Precipitation Through The Genetic Fusion Of Charged Polypeptides To Enzymes , Mark Quinn Niederauer

Retrospective Theses and Dissertations

The focus of this work was to investigate the enhancement of polyelectrolyte precipitation through the genetic alteration of the target protein. The dissertation is divided into three primary chapters: a literature review of precipitation techniques which display high selectivity, experimental work including the characterization and precipitation of the fusion enzymes, and modeling of the precipitation process;The literature review of selective precipitation techniques focuses on those techniques for which relatively high purifications can be achieved. The techniques include precipitation with polyelectrolytes, affinity ligands, metal ions, and protein-binding dyes. Topics discussed include the mechanisms of selectivity and precipitation, degrees of purification ...