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Full-Text Articles in Engineering
A Platform For Fast Detection Of Let-7 Micro Rna Using Polyaniline Fluorescence And Image Analysis Techniques, Partha P. Sengupta
A Platform For Fast Detection Of Let-7 Micro Rna Using Polyaniline Fluorescence And Image Analysis Techniques, Partha P. Sengupta
Master's Theses
The project describes a new strategy for transducing hybridization events through modulating intrinsic properties of the electroconductive polymer polyaniline (PANI). When DNA based probes electrostatically interact with PANI, its fluorescence properties are increased, a phenomenon that can be enhanced by UV irradiation. Hybridization of target nucleic acids results in dissociation of probes causing PANI fluorescence to return to basal levels. By monitoring restoration of base PANI fluorescence as little as 10-11 M (10 pM) of target oligonucleotides could be detected within 15 minutes of hybridization. Detection of complementary oligos was specific, with introduction of a single mismatch failing to …
Development Of A High Precision Quantum Dot Synthesis Method Utilizing A Microfluidic Reactor And In-Line Fluorescence Flow Cell, William Henry Lafferty
Development Of A High Precision Quantum Dot Synthesis Method Utilizing A Microfluidic Reactor And In-Line Fluorescence Flow Cell, William Henry Lafferty
Master's Theses
Quantum dots show great potential for use as spectral converters in solar cells, lighting applications, and biological imaging. These applications require precise control of quantum dot size to maximize performance. The quality, size, and fluorescence of quantum dots depend on parameters that are difficult to control using traditional batch synthesis processes. An alternative, high precision process was developed for the synthesis of cadmium-selenide quantum dots using a microfluidic reactor and fluorescence flow cell. The process required creating separate cadmium and selenium precursors that were then mixed in a nitrogen environment at 17°C. Using an NE-300® syringe pump, the solution …
The Design And Fabrication Of A Microfluidic Reactor For Synthesis Of Cadmium Selenide Quantum Dots Using Silicon And Glass Substrates, Peter Robert Gonsalves
The Design And Fabrication Of A Microfluidic Reactor For Synthesis Of Cadmium Selenide Quantum Dots Using Silicon And Glass Substrates, Peter Robert Gonsalves
Master's Theses
A microfluidic reactor for synthesizing cadmium selenide (CdSe) quantum dots (QDs) was synthesized out of a silicon wafer and Pyrex glass. Microfabrication techniques were used to etch channels into the silicon wafer. Holes were wet-drilled into the Pyrex glass using a diamond-tip drill bit. The Pyrex wafer was anodically bonded to the etched silicon wafer to enclose the microfluidic reactor. Conditions for anodic bonding were created by exposing the stacked substrates to 300V at ~350oC under 5.46N of force. A syringe containing a room temperature CdSe solution was interfaced to the microfluidic reactor by using Poly (dimethylsiloxane) (PDMS) as an …
Comprehensive Analysis Of Effluent Organic Matter From Five Wastewater Treatment Plants In Connecticut And Comparison To Natural Organic Matter, Matthew L. Quaranta
Comprehensive Analysis Of Effluent Organic Matter From Five Wastewater Treatment Plants In Connecticut And Comparison To Natural Organic Matter, Matthew L. Quaranta
Master's Theses
Natural organic matter is an important driver of biotic and abiotic processes in aquatic environments. Wastewater treatment plants discharge a substantial amount of organic matter into the environment; however effluent organic matter has not been well studied. In this study, traditional organic geochemical techniques were applied to characterize effluent organic matter. Effluent organic matter was isolated by DAX8 (hydrophobic fraction) and XAD4 (transphilic fraction) Amberlite resins. Extraction efficiencies of effluent organic matter by DAX8 resins ranged from 18 to 42 percent as a result of larger content of hydrophilic organic matter than natural organic matter. Average organic matter molecular weights …
Characterization And Implementation Of Low Intensity Pulsed Ultrasound As A Tool To Apply Physical Load To Scaffolds And Bone Cells For Fracture Repair, Scott Frazee
Master's Theses
One current challenge in treating bone fractures is the effective treatment of non-unions and delayed unions. Low Intensity Pulsed Ultrasound (LIPUS) has been approved by the FDA to treat fresh fractures since 1994 and non-unions since 2000 and is an attractive treatment option because it is non-invasive. The mechanism by which it works, however, is not well understood; what is known is largely confined to the resultant changes in chemical output of cells. In this thesis several concepts and techniques were brought together to investigate the following hypothesis: LIPUS produces a measurable physical load that results in measurable deformation and …