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Full-Text Articles in Engineering

Bioluminescence Resonance Energy Transfer (Bret) - Based Nanostructured Biosensor For Detection Of Glucose, Eugene Hwang Nov 2019

Bioluminescence Resonance Energy Transfer (Bret) - Based Nanostructured Biosensor For Detection Of Glucose, Eugene Hwang

Electronic Thesis and Dissertation Repository

Bioluminescence resonance energy transfer (BRET) is a distance dependent, non-radiative energy transfer, which uses a bioluminescent protein to excite an acceptor through resonance energy transfer. In this thesis, BRET technology is incorporated into a sensor comprised of a recombinant protein and quantum dots. The recombinant protein, which includes the bioluminescent protein, Renilla luciferase (Rluc), is used as the donor molecule and cadmium tellurium quantum dots as the acceptor molecules. Separating the donor-acceptor pair is a recombinant protein, glucose binding protein, which changes conformation upon binding glucose and brings the pair closer together, thus allowing BRET to occur. Optimization of the ...


Evaluation Of Biohydrogen Production From Co-Fermentation Of Carbohydrates And Proteins, Emmanuel Andrew Tepari Jr, Sep 2019

Evaluation Of Biohydrogen Production From Co-Fermentation Of Carbohydrates And Proteins, Emmanuel Andrew Tepari Jr,

Electronic Thesis and Dissertation Repository

The aim of this study was to examine volatile fatty acid (VFA) production from a proteinaceous substrate, bovine serum albumin (BSA) for a pH range of 5 – 9, and to further assess its impact on hydrogen production in a co-fermentation process using starch and BSA at different ratios. The established optimum conditions for VFA production from BSA were an initial pH of 8, incubation time of 3 days and an operating temperature of 37 ℃. Using these fermentation conditions, the stoichiometric reactions that describe the anaerobic degradation of BSA were investigated. A methodology that describes organic acid production from BSA by ...


Characterization Of Tetrazolium Salts And Formazans Using Computational Chemistry For Radiochromic Dosimetry, Teshome G. Bedada Jul 2019

Characterization Of Tetrazolium Salts And Formazans Using Computational Chemistry For Radiochromic Dosimetry, Teshome G. Bedada

Electronic Thesis and Dissertation Repository

Computational chemistry is a powerful tool to model different materials and predict their physical and chemical properties. The goal of this thesis was to apply different computational chemistry tools to model the properties of two classes of closely related organic compounds: formazans and tetrazolium salts for ionizing radiation dosimetry in water-based gels, whereby the absorbed dose is registered by optical detection of formazan formation from tetrazolium salts. The structural parameters of phenyl and benzothiazolyl-substituted tetrazolium salts and formazans were computed using the APFD density functional theory (DFT) model and 6-311+G(2d,p) basis set. Conformational searching of stereoisomers (TSCA ...


Anaerobic Digestion Of Aqueous Pyrolysis Condensate, Connie Wen Aug 2018

Anaerobic Digestion Of Aqueous Pyrolysis Condensate, Connie Wen

Electronic Thesis and Dissertation Repository

Lignocellulosic material can be subjected to pyrolysis to yield pyrolysis liquid, biochar, and gas. The pyrolysis liquid resulting from the condensation of vapours can be separated into a rich organic condensate and an acidic “aqueous pyrolysis condensate” (APC) which may be considered a waste. The target of this research is subjecting APC to anaerobic digestion to produce biogas that can be energy purposed, and to reduce the organic load of APC to acceptable levels for possible disposal. From this study, it was found that without any treatment, the inoculum requires 50 to 60 days of adaptation period to reduce the ...


Maintaining And Controlling An Extrinsic Biofilm For Pathogen Removal In Dental Unit Water Lines, Bilal B. Al-Bataina Apr 2018

Maintaining And Controlling An Extrinsic Biofilm For Pathogen Removal In Dental Unit Water Lines, Bilal B. Al-Bataina

Electronic Thesis and Dissertation Repository

The control of biofilm formation is a major concern for industrial, environmental, and public health. Undesirable biofilms can harbor different disease-causing pathogens and shorten the operational life of different equipment. On the other hand, beneficial biofilms are also used in various applications and managing its growth and activity can be desirable. Killing the biofilm does not usually incorporate the removal of the dead biofilm structure that is adhered to the surface. Therefore, the aim of this thesis is to control biofilm formation; to be able to remove, inhibit, and enhance biofilm formation. This thesis investigated the use of norspermidine, D-amino ...


Biobutanol Production From Cellulosic And Sugar-Based Feedstock From The Corn Plant, Reyna Gomez-Flores Apr 2018

Biobutanol Production From Cellulosic And Sugar-Based Feedstock From The Corn Plant, Reyna Gomez-Flores

Electronic Thesis and Dissertation Repository

In this thesis, biobutanol production by biological fermentation was investigated from the corn plant, integrating two approaches. The first one was to utilize corn cobs, a cellulosic-based material. The second, using a new sugar-based material, sugarcorn juice. Utilizing suitable Clostridia strains for each substrate, these approaches converged into a biorefinery concept to produce renewable biofuels in Ontario, Canada. The corn cob pretreatment was carried out by a dilute acid method resulting in temperature as the variable with most significant effect towards glucose liberation. The enzymatic hydrolysis was performed utilizing a very low concentration of an enzymatic stock solution with approximately ...


Hydrothermal Liquefaction Of Microalgae For The Production Of Bio-Crude Oil, Yulin Hu Apr 2018

Hydrothermal Liquefaction Of Microalgae For The Production Of Bio-Crude Oil, Yulin Hu

Electronic Thesis and Dissertation Repository

Due to the depletion of fossil fuels and climate change, extensive research has performed towards renewable energy production from microalgal biomass. Microalgae have several inherent benefits, such as high photosynthetic efficiencies, fast growth rates, and high lipid contents. In addition, microalgae can be cultivated in the non-arable lands (e.g., saline and waste water), thereby no competition with food crops production.

Transesterification is the one of commonly used technologies for converting microalgae into liquid bio-fuels (i.e., biodiesel). Normally, an energy-intensive drying step is required in the transesterification treatment, which accounts for nearly half of the energy input. Hence, if ...


Covalently Crosslinked Organic/Inorganic Hybrid Biomaterials For Bone Tissue Engineering Applications, Dibakar Mondal Feb 2018

Covalently Crosslinked Organic/Inorganic Hybrid Biomaterials For Bone Tissue Engineering Applications, Dibakar Mondal

Electronic Thesis and Dissertation Repository

Scaffolds are key components for bone tissue engineering and regeneration. They guide new bone formation by mimicking bone extracellular matrix for cell recruitment and proliferation. Ideally, scaffolds for bone tissue engineering need to be osteoconductive, osteoinductive, porous, degradable and mechanically competent. As a single material can not provide all these requirements, composites of several biomaterials are viable solutions to combine various properties. However, conventional composites fail to fulfil these requirements due to their distinct phases at the microscopic level. Organic/inorganic (O/I) class II hybrid biomaterials, where the organic and inorganic phases are chemically crosslinked on a molecular scale ...


Bacterial Cellulose Nanocrystals: Production And Application, Isabela Reiniati Aug 2017

Bacterial Cellulose Nanocrystals: Production And Application, Isabela Reiniati

Electronic Thesis and Dissertation Repository

The aims of this study were to investigate the effect of culture conditions on the production of bacterial cellulose (BC) by Komagataeibacter xylinus (K. xylinus), to assess the feasibility of tailoring the surface properties of bacterial cellulose nanocrystals (BCNs) through the culture conditions, and to use the BCNs in an aqueous system for drug adsorption application. BC fibers production improved with increased agitation rates in a stirred tank bioreactor resulting in yields of 0.54 and 1.13 g of BC per litre at agitation rates of 500 rpm and 700 rpm, respectively. Separation and purification of bacterial cellulose were ...


Bubble-Induced Inverse Gas-Liquid-Solid Fluidized Bed, Xiliang Sun Aug 2017

Bubble-Induced Inverse Gas-Liquid-Solid Fluidized Bed, Xiliang Sun

Electronic Thesis and Dissertation Repository

Gas-liquid-solid fluidized beds have been widely applied in wastewater treatment, however, the current method of wastewater process has several limitations. Hence, an improved method is in demand. A 3.5 height and 0.1534m inner diameter column was used to study the hydrodynamic characteristics of a bubble-induced three-phase inverse fluidized bed. Air, water and three types of low-density particles were employed as gas, liquid and solid phases.

The hydrodynamic properties in the bubble-induced three-phase fluidized bed were investigated to provide the basic information for the industrial process, such as flow regime, bed expansion ratio and phase holdups. A flow regime ...


Fabrication And Characterization Of Hybrid Nanocomposites By Matrix Assisted Pulsed Laser Evaporation, Songlin Yang Aug 2017

Fabrication And Characterization Of Hybrid Nanocomposites By Matrix Assisted Pulsed Laser Evaporation, Songlin Yang

Electronic Thesis and Dissertation Repository

Different methods have been applied to deposit hybrid nanocomposites which can be applied in various fields due to their light weight and multifunctional properties. Here, matrix assisted pulsed laser evaporation (MAPLE) equipment with 532 nm Nd:YAG laser is applied to fabricate three types of hybrid nanocomposites on different substrates.

Chemical synthesized FeCo nanoparticles were deposited on graphene sheets by MAPLE technique (laser fluence: 300 mJ/cm2). The effects of deposition time (t) on particle amount, shape and size have been investigated. Yttrium barium copper oxide (YBCO) materials are one type of high-temperature superconductive materials and could be applied ...


Nanostructured Biosensor For Tear Glucose Detection Based On Bioluminescence Resonance Energy Transfer (Bret) Mechanism., Denghuang Zhan Aug 2017

Nanostructured Biosensor For Tear Glucose Detection Based On Bioluminescence Resonance Energy Transfer (Bret) Mechanism., Denghuang Zhan

Electronic Thesis and Dissertation Repository

Bioluminescence Resonance Energy Transfer (BRET), a sensitive, non-destructive and self-illuminated method, has been now commonly used to test protein interactions. Here, we describe a BRET sensor for non-invasively detecting glucose molecules. The sensor is made by the bioconjugation of quantum dots and recombinant protein. The recombinant protein contains the bacterial glucose binding protein (GBP) and a bioluminescent protein, Renilla luciferase (Rluc), used as the donor with the emission peak at 470 nm, which is able to excite the acceptor of BRET sensor made of cadmium tellurium quantum dots ( CdTe QDs) with the emission peak at 570 nm. The distance between ...


Genetic Engineering Studies Of Escherichia Coli And Microalgae For Expression Of Hydrolytic Enzymes And Development Of High Throughput Screening Technique, Shreyas S. Yedahalli Jul 2017

Genetic Engineering Studies Of Escherichia Coli And Microalgae For Expression Of Hydrolytic Enzymes And Development Of High Throughput Screening Technique, Shreyas S. Yedahalli

Electronic Thesis and Dissertation Repository

The field of biochemical engineering has made substantial progress through major advances in genetic and metabolic engineering with applications in various sectors such as energy, food science, pharmaceuticals, etc. The hosts used for this work are constantly broadening. A host particularly important for energy applications are microalgae. The potential to enhance microalgae genetically for energy applications is not well explored and was therefore investigated in this thesis. Non-photosynthetic micro-organisms and photosynthetic microalgae offer a potential approach to enhance sustainable biochemical production. In this study expression vectors for Escherichia coli (E. coli) and Chlorella vulgaris (C. vulgaris) were constructed for the ...


Investigation Of Lutein Production And Recovery From Chlorella Vulgaris Using Phototrophic Cultivation, Mengyue Gong Jul 2017

Investigation Of Lutein Production And Recovery From Chlorella Vulgaris Using Phototrophic Cultivation, Mengyue Gong

Electronic Thesis and Dissertation Repository

Microalgae have been recognized as a natural reservoir of the valuable commercial carotenoid lutein due to its high lutein content and fast growth cycle. However, the production of lutein from microalgal sources is not yet sufficiently cost-effective to compete with traditional marigold flower-based processing (Park et al., 2015). This thesis aims to investigate the factors affecting lutein production and recovery from microalgae using a phototrophic cultivation mode.

The closed photobioreactors can offer controlled conditions for faster microalgae growth. A coiled tubular tree photobioreactor (CTPBR) design was first investigated for cultivating the cold tolerant microalgae, Chlorella vulgaris UTEX 265, under various ...


Bioethanol Production Using Saccharomyces Cerevisae Cultivated In Sugarcorn Juice, Thirumalai Nambi Thiruvengadathan Jun 2017

Bioethanol Production Using Saccharomyces Cerevisae Cultivated In Sugarcorn Juice, Thirumalai Nambi Thiruvengadathan

Electronic Thesis and Dissertation Repository

For the first time, juice extracted from sugarcorn, a new Canadian energy crop, was used for bioethanol production. Physical and chemical characteristics of sugarcorn juice (SCJ) were determined. SCJ contained a maximum of 145 g/L of carbohydrates, with sucrose, glucose and fructose together contributing 80%. Effect of autoclaving and carbon filtration on juice sugars were investigated.

Shake flask fermentations using Saccharomyces cerevisiae grown in yeast extract supplemented SCJ produced a maximum of 45.6 g/L ethanol in 72 h. Bioreactor studies using un-supplemented SCJ achieved 40 g/L ethanol in 26 h, yielding a maximum of 0.46 ...


Magnetic Stimulation On The Growth Of The Microalga Nannochloropsis Oculata, Manuella Oliveira Jun 2017

Magnetic Stimulation On The Growth Of The Microalga Nannochloropsis Oculata, Manuella Oliveira

Electronic Thesis and Dissertation Repository

Fossil fuels, our principal sources of energy supply, are non-renewable and research is needed on alternatives that are renewable and potentially more environmentally friendly. Microalgae have been investigated as a future feedstock alternative to petroleum but the technology is still expensive and improvements are needed. Reduction in costs might be achieved by increasing algal biomass and lipid productivity. The lipids can be used to produce biofuels such as biodiesel and biojet fuel. The marine microalga Nannochloropsis oculata grows well and can accumulate high lipid content. In this study, the effects of static magnetic field stimulation (SMF) of 0 (control), 5 ...


Disinfection By-Products Formation After Coagulation Of Algal Extracellular And Intracellular Organic Matters, Tianyiru Chen Feb 2017

Disinfection By-Products Formation After Coagulation Of Algal Extracellular And Intracellular Organic Matters, Tianyiru Chen

Electronic Thesis and Dissertation Repository

Algal organic matter (AOM) is found in high concentration during algal bloom season in drinking water systems, which is generally categorized into extracellular organic matter (EOM) and intracellular organic matter (IOM). These compounds are not well removed in traditional water treatment methods such as coagulation, and are the precursors of subsequent disinfection by-products (DBPs) during chlorination of water. In this study, EOM and IOM content of four different algae were quantified measuring dissolved organic carbon (DOC), UV absorbance at 254 nm and turbidity. Coagulation using alum (Al2(SO4)3•16H2O)was used to remove the algal ...


Co-Substrate Fermentation Of Jerusalem Artichoke Tubers And Crude Glycerol To Butanol With Integrated Product Recovery, Tahereh Sarchami Nov 2016

Co-Substrate Fermentation Of Jerusalem Artichoke Tubers And Crude Glycerol To Butanol With Integrated Product Recovery, Tahereh Sarchami

Electronic Thesis and Dissertation Repository

Butanol has long been considered a potential advanced liquid biofuel, in addition to its current application as an industrial solvent. It can be produced biologically; however, the conventional ABE fermentation suffers from many limitations, including low butanol titer, high cost of traditional raw materials, end-product inhibition and high butanol recovery costs. Possible solutions are the use of renewable low-cost feedstocks, genetic manipulations of Clostridia spp. to improve the strains’ butanol titer and tolerance, advanced fermentation techniques, and in-situ product recovery technologies.

In order to overcome some of these limitations, the overall goal of this thesis was to develop a process ...


Fabrication And Cross-Linking Of L-Aspartic Acid Functionalized Poly(Ester Amide)-Based Tissue Engineering Scaffolds, Shuyu Liu Sep 2016

Fabrication And Cross-Linking Of L-Aspartic Acid Functionalized Poly(Ester Amide)-Based Tissue Engineering Scaffolds, Shuyu Liu

Electronic Thesis and Dissertation Repository

Scaffold fabrication is essential in tissue engineering. Amino acid based poly(ester amide)s (PEAs) have been investigated as scaffold materials due to their non-toxic degradation byproducts, and easily tunable mechanical and biological properties. However, L-aspartic acid functionalized PEAs showed poor morphological stability when immersed in buffer. This work focuses on the further functionalization of L-aspartic acid based PEAs, scaffold fabrication and cross-linking of the scaffold to improve morphological stability. Photo-cross-linkable and thermally cross-linkable PEAs were synthesized successfully and characterized. Two scaffold fabrication methods were applied: 1) electrospinning was applied to photo-cross-linkable PEAs, followed by UV treatment to cross-link electrospun ...


Abe Fermentation From Low Cost Substrates, Kai Gao Sep 2016

Abe Fermentation From Low Cost Substrates, Kai Gao

Electronic Thesis and Dissertation Repository

The high cost of substrate and product inhibition in the fermentation broth remains two major problems associated with bio-butanol production. This thesis aims to solve these problems by examining abundant lignocellulosic biomass as potential feedstocks and exploring novel substrates such as carbohydrates derived from microalgae for ABE fermentation. The commonly observed toxic effect after pretreatment of lignocelluslosic biomass was removed by resin adsorption, where the resin could also serve as an in-situ butanol recvoery devices.

Corn cobs (an agricultural waste), switchgrass (an energy crop) and phragmites (an invasive plant in North America) were investigated as substrates for ABE fermentation by ...


Biohydrogen And Biomethane Production From Lignocellulosic Biomass, Chinaza Okeoghene Akobi Aug 2016

Biohydrogen And Biomethane Production From Lignocellulosic Biomass, Chinaza Okeoghene Akobi

Electronic Thesis and Dissertation Repository

The main purpose of this study was to investigate the impact of furfural on mixed cultures during fermentative hydrogen production from lignocellulosic biomass. Small batch studies using synthetic lignocellulosic hydrolysate grown on mesophilic mixed cultures, revealed a threshold furfural concentration of greater than 1 g/L with enhancement to the yields (from the control) observed at 0.5 g/L furfural (at initial substrate-to–biomass (S°/X°) ratios of 0.5 and 1 gCOD/gVSS) and at both 0.5 g/L and 1 g/L furfural (at S°/X° of 2 and 4 gCOD/gVSS). This study was scaled-up ...


Direct Conversion Of Wet Microalgae And Oleaginous Yeast To Biodiesel Using Ionic Liquids, Valerie Orr Aug 2016

Direct Conversion Of Wet Microalgae And Oleaginous Yeast To Biodiesel Using Ionic Liquids, Valerie Orr

Electronic Thesis and Dissertation Repository

This thesis examined the development of an oleaginous yeast or microalgae based biorefinery process. First, improvements were made to the Nile Red assay, a high-throughput method for monitoring lipid accumulation in oleaginous organisms resulting in a significantly more reproducible and accurate assay. This assay was then used to optimize lipid production during heterotrophic cultivation of microalgae on glucose using response surface methodology resulting in microalgae with high lipid content (37.6% wt.). In order to improve the renewability of heterotrophic lipid production, both oleaginous yeast and microalgae were cultivated using pyrolytic sugars, produced via fast pyrolysis of pinewood waste. The ...


Hydrothermal Liquefaction Of High-Water Content Biomass And Waste Materials For The Production Of Biogas And Bio-Crude Oil, Laleh Nazari Aug 2016

Hydrothermal Liquefaction Of High-Water Content Biomass And Waste Materials For The Production Of Biogas And Bio-Crude Oil, Laleh Nazari

Electronic Thesis and Dissertation Repository

Growing interest in renewable energies due to shrinking reserves of fossil fuels and climate change concerns have led to extensive research towards gaseous and liquid fuels production from renewable energy resources such as biomass and wastes. Energy generation from municipal and industrial wastes such as wastewater sludge is also environmental friendly way to deal with large volume of waste disposal with the additional advantage of eliminating part of the indirect greenhouse gas emissions from energy crops-derived biofuels.

In this thesis, a novel process for co-production of biogas and bio-crude oil from high-water-content wastewater sludge through hydrothermal liquefaction (HTL) treatments is ...


Studies On Nanocomposite Coating Produced By Laser-Assisted Process To Prevent Silicone Hydrogels From Bio-Fouling, Vishnuvardhana Wuppaladhodi Aug 2016

Studies On Nanocomposite Coating Produced By Laser-Assisted Process To Prevent Silicone Hydrogels From Bio-Fouling, Vishnuvardhana Wuppaladhodi

Electronic Thesis and Dissertation Repository

In this thesis, silver nanoparticles incorporated into polyvinylpyrrolidone (PVP) were deposited on silicone hydrogel to improve the hydrophilicity of the silicone hydrogel and prevent the growth of bacteria. Two different processes were employed to produce Ag nanoparticles: (1) Process-A is a photochemical reduction; (2) Process-B is laser ablation in liquid. Following that, MAPLE process was employed to deposit the Ag-PVP nanocomposites on the surface of silicone hydrogel. A solid-state pulsed laser (Nd:YAG) with a wavelength of 532 nm at a fluence of 50.4 mJ/cm2 was used in the MAPLE system to deposit Ag-PVP nanocomposite coating. Our ...


Fermentative Processes Requiring Low Solubility Feed Gases: An Investigation Into Gas-Dependent Microorganisms, Eric W. Doerr Jul 2016

Fermentative Processes Requiring Low Solubility Feed Gases: An Investigation Into Gas-Dependent Microorganisms, Eric W. Doerr

Electronic Thesis and Dissertation Repository

Two bioprocesses were separately investigated based on their common interest of using gaseous substrates that have low solubility. The first process involved the development of a mixed culture using two organisms capable of utilizing glycerol and carbon monoxide separately to increase biobutanol production, while the second process involved an investigation of different production media used in aerobic xanthan production of Xanthomonas campestris with pressurization effects. It was determined that Clostridium pasteurianum should be used with an organism like Clostridium carboxidivorans or Eubacterium limosum in order for butyrate uptake at 3 g/L to occur with a minimum 0.1 g ...


Minor Whey Protein Purification Using Ion-Exchange Column Chromatography, Naeimeh Faraji Dizaji Apr 2016

Minor Whey Protein Purification Using Ion-Exchange Column Chromatography, Naeimeh Faraji Dizaji

Electronic Thesis and Dissertation Repository

This thesis is concerned with application of mechanistic models for recovery and purification of two minor milk proteins to develop an efficient and robust process. A fundamental and quantitative understanding of the underlying mechanisms assists to evaluate chances and challenges in non-linear chromatography.

The first chapter considers adsorption isotherm data of two minor whey proteins on cation exchanger under various conditions and used as the basis to develop a predictive approach for correlating adsorption behavior using a mechanistic isotherm model. The SMA isotherm model explicitly considers the contributions of protein-adsorbent and protein-protein interactions in the simulation of salt gradients in ...


Recovery Of Valuable Chemicals From Agricultural Waste Through Pyrolysis, Mohammad M. Hossain Feb 2016

Recovery Of Valuable Chemicals From Agricultural Waste Through Pyrolysis, Mohammad M. Hossain

Electronic Thesis and Dissertation Repository

Agricultural crop residues are a source of inexpensive biomass to convert into bioproducts. The recovery of valuable chemicals from plant waste would partly solve the disposal issue and offer a more environmentally friendly alternative to synthetic chemical production. One approach to separate and concentrate valuable chemicals from biomass is pyrolysis using a batch reactor process. A mechanically fluidized reactor (MFR) was developed to pyrolyze biomass from ambient to temperatures near 600 °C, forming gases that are then condensed in an ice-chilled condenser to form a bio-oil. The bio-oil produced by the MFR can be separated within temperature ranges, termed one-dimensional ...


A Study Of Low C/N Ratio Waste Water Treatment Using Fluidized Bed Bioreactor, Kai Li Feb 2016

A Study Of Low C/N Ratio Waste Water Treatment Using Fluidized Bed Bioreactor, Kai Li

Electronic Thesis and Dissertation Repository

In wastewater treatment field, one of the severe challenges is the handling of ammonia-rich wastewater with a low C/N ratio. The failure of nitrogen treatment can lead to adverse ecological and biological effects including depletion of dissolved oxygen, eutrophication, toxicity to aquatic life, and reduction in the suitability of water for reuse. Conventionally, extra organic carbon dosing is required in treating low C/N wastewater. Alternately, partial nitrification is of interest as an emerging technology for its’ lesser need of organic carbon addition and cost savings in aeration. In this study, a lab-scale partial nitrification fluidized bed bioreactor (PNFBR ...


Polymeric Superparamagnetic Nanoparticles For Drug Delivery Applications, Leena Mohammed Feb 2016

Polymeric Superparamagnetic Nanoparticles For Drug Delivery Applications, Leena Mohammed

Electronic Thesis and Dissertation Repository

Drug delivery systems based on magnetic nanoparticles present a promising avenue for controlled targeted therapeutics, especially in cancer therapy. Conventional systematic therapeutics encompasses numerous side effects due to its limited selectivity between healthy and cancerous cells. In this thesis, novel polymeric-metallic hybrid nanoaggregates were developed to address this challenge. Magnetite nanoparticles were synthesized via precipitation of iron oxide and was surface modified using a unique chitosan derivative, glycol chitosan (GC), loaded with progesterone for potential hormonal therapy application for breast cancer. Surface characterizations techniques, in vitro drug release kinetics, investigation of progesterone release mechanism by mathematical modeling, and cell cytotoxicity ...


Polyhydroxybutyrate Production In A Photobioreactor Using Spirulina Platensis, Adrianna Mika Dec 2015

Polyhydroxybutyrate Production In A Photobioreactor Using Spirulina Platensis, Adrianna Mika

Electronic Thesis and Dissertation Repository

Biopolymers and other naturally derived chemicals continue to gain attention over petrochemical based products due to the price volatility of crude oil, increasing need to reduce greenhouse gas emissions and public awareness of environmental issues. Cyanobacterium Spirulina (Arthrospira) platensis, a commercially feasible blue-green alga, is able to synthesize poly(3-hydroxybutyrate) (PHB), a biodegradable and biocompatible polymer, as an energy storage product of photosynthesis directly from CO2. A flat plate photobioreactor (PBR) with LED illumination was designed for the cultivation of S. platensis. Computational fluid dynamics (CFD) software was used to improve airflow uniformity and mixing in the photobioreactor. PHB ...