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Full-Text Articles in Engineering
Human Hair Keratin Protein, Hair Fibers And Hydroxyapatite (Ha) Composite Scaffold For Bone Tissue Regeneration, Samuel Siyum
Human Hair Keratin Protein, Hair Fibers And Hydroxyapatite (Ha) Composite Scaffold For Bone Tissue Regeneration, Samuel Siyum
ETD Archive
The field of tissue engineering aims at promoting the regeneration of tissues or replacement of failing or malfunctioning tissue by means of combining a scaffold material, adequate cells and bioactive molecules. Different materials have been proposed for use as three-dimensional porous scaffolds for bone tissue engineering procedures. Among them, polymers of natural origin are one of the most attractive options mainly due to their similarities with the extracellular matrix (ECM), chemical versatility as well as typically good biological performance. In this study, two biocompatible composite scaffolds were developed from natural polymer by tissue engineering approach and tested in vitro. The …
One-Dimensional Radial Diffusion Of Small Molecules (376 Da) In Bone Tissue, Kurt W. Farrell
One-Dimensional Radial Diffusion Of Small Molecules (376 Da) In Bone Tissue, Kurt W. Farrell
ETD Archive
The flow of nutrients through any biological tissue is important to maintain homeostasis. If the transport process is understood, medical research teams can better design medications, prosthetic implants, and tissue scaffolds. Additionally, transport rates help physicians to better understand disease states and wound healing, including minor injuries such as breaks and sprains, which will aid in better diagnoses. We developed a novel method that measures the rate of diffusion in vitro, of fluorescein sodium salt. Samples were incubated at 37°C in a 5 CO2 atmosphere for various periods of time. Samples were sliced and analyzed using Image-Pro Plus and MATLAB …
Design, Development And Biomechanical Analysis Of Scaffolds For Augmentation Of Rotator Cuff Repairs, Amit Aurora
Design, Development And Biomechanical Analysis Of Scaffolds For Augmentation Of Rotator Cuff Repairs, Amit Aurora
ETD Archive
Rotator cuff tears are a source of debilitating pain that commonly affects more than 40 of our aging population. Despite advances in surgical treatment, the failure rate of rotator cuff repairs is as high as 20-90 . Extracellular matrix (ECM) derived scaffolds have recently been investigated as augmentation devices for rotator cuff repairs, but none has yet demonstrated both the appropriate biological and mechanical properties for mitigating re-tears and enhancing healing. This dissertation proposes to engineer the mechanical properties of allograft fascia lata in a manner that will allow its use as an augmentation device for rotator cuff repairs. This …
Surface Microtopography Modulation Of Biomaterials For Bone Tissue Engineering Applications, Eun Jung Kim
Surface Microtopography Modulation Of Biomaterials For Bone Tissue Engineering Applications, Eun Jung Kim
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The ultimate goal of this project is to develop a biodegradable and implantable scaffold with precise surface topographies that can provide osteoconductive stimuli to connective tissue progenitor cells (CTPs), and subsequently, enhance bone regeneration applications without the complications of autogenous cancellous bone grafts. This dissertation presents the modification of surface microtextures to provide osteoconductive stimuli to CTPs for bone regeneration applications. First, the effect of surface topography on cell proliferation and osteogenic differentiation was validated through experiments using surface post microtextures and CTPs. Post microtextures accelerated CTP growth behaviors compared to smooth polydimethylsiloxane (PDMS) and standard cell culture dishes. Second, …
Imaging Of Tyramine-Substituted Hydrogels For Tissue Replacement, Ediuska V. Laurens
Imaging Of Tyramine-Substituted Hydrogels For Tissue Replacement, Ediuska V. Laurens
ETD Archive
Novel tyramine-based hyaluronan (HA) and collagen hydrogels have been developed in which cross-linking is accomplished via peroxidase-mediated dityramine linkages allowing direct cross-linking in vivo. These TB hydrogels possess advantageous physical properties, which include excellent biocompatibility and the ability to mimic the biological, structural and mechanical properties of normal, healthy tissues, including cartilage, and thus provide for synthetic, implantable biomaterials suitable for a wide range of tissue types. The efficacy of these TB-hydrogels has been previously tested in a number of clinically relevant animal models, which have evaluated their applicability for the repair/replacement of various tissues, including cartilage. Nevertheless, there exists …
Cortical Bone Tissue Engineering;Scaffold Design And Cell Selection, Demin Wen
Cortical Bone Tissue Engineering;Scaffold Design And Cell Selection, Demin Wen
ETD Archive
Cortical bone tissue engineering provides a promising approach to generate graft materials needed to treat the large sized bone defects. The underling premise of tissue engineering is to mimic the in vivo microenvironment as best as possible in vitro culture system. To select an appropriate scaffold material used in this model system, mechanical and hydraulic permeability properties of 316L porous stainless steel and polymethylmethacrylate (PMMA) were studied, as well as their biocompatibility in short and long term cell culture. Results showed that scaffolds made of both stainless steel and PMMA: (1) could be manufactured to have similar permeability as that …