Engineering Commons^™

Investigation Of The Tailoring Steps In Pradimicin Biosynthesis, Kandy L. Napan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research focused on the investigation of the late steps in the biosynthetic pathway of the novel antifungal and antiviral pradimicins A-C. Pradimicins were first isolated from the soil bacterium Actinomadura hibisca. These bioactive molecules are assembled by a type II polyketide biosynthetic pathway. Although the biosynthetic gene cluster of pradimicin has been identified, the functions of the biosynthetic genes and how they work collaboratively to form the final structures of pradimicins remain unknown. This research aims to functionally characterize the enzymes involved in the late steps of the biosynthetic route.

The early biosynthetic steps of pradimicins have been …

Characterization And Application Of Dynamic In Vitro Models Of Human Airway, Hemangkumar J. Patel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In recent years, respiratory diseases have emerged as a leading cause of mortality across the globe. In the United States alone respiratory diseases are the fourth leading cause of deaths annually. Moreover, with the rapid increase of industrialization and urbanization, the occurrences of respiratory diseases are expected to remain high with strong chances of increasing in the future. To ameliorate the epidemic of respiratory disease, it is first important to understand its underlying mechanisms.

Respiratory research studies in animals have elucidated the chronological order of the pathological events and systemic responses inside the lung, but understanding the response of individual …

The Transfer Of Agricultural Water To Municipal And Industrial Usages, Dallin Paul Stephens

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The water that is available for beneficial use in Utah is quickly approaching full appropriation; water that has been claimed is nearing the amount that is available for use. The Division of Water Resources of the State of Utah has organized a three-part plan to "Plan, Conserve, Develop and Protect Utah's Water Resources." One of these three elements has a focus to "provide comprehensive water planning." Such planning is best achieved when current and accurate data on the uses of the state's water are available.

The primary purpose of this thesis was to provide an evaluation, from data collected on …

A Study On The Applications And Toxicity Assessments Of Carbon Nanotubes In Tissue Engineering, Rena Baktur

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Carbon nanotubes (CNTs) are one of the most popular nanomaterials. There has been increasing interest in the development and applications of carbon nanotubes due to their huge potential in industrial and medical applications. Recent applications of carbon nanotubes include development of scaffolds and drug delivery systems. Despite rapidly emerging applications of CNTs, little is known about the impact of CNTs on cellular processes, especially mesenchymal stem cell (MSC)'s differentiation. Also, the effects of nanoparticle exposure under different conditions on cellular responses have not been well characterized yet.

To characterize the effects of CNTs on creating nanoscale scaffolds for tissue engineering, …

The Hypericum Perforatum Herb As An Antimycobacterial Agent And Its Implications As An Additional Tuberculosis Medication, Trent W. Mortensen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An immediate demand exists for new tuberculosis (TB) antibiotics due to the ever-increasing spread of drug-resistant strains. The drug-development process goes through four phases, the first (Phase 0) of which is to demonstrate and investigate drug effectiveness and toxicity. This research investigated the effectiveness of the Hypericum perforatum herb (commonly St. John's wort (SJW)) in its growth inhibition of mycobacteria and its viability effect on human lung cells.

Organic-solvent SJW extracts were effective at inhibiting every nonpathogenic genetically sequenced Mycobacterium isolate currently available (six isolates) in preliminary studies. Quantitative studies of five Mycobacterium isolates showed an order of concentration sensitivity …

Characterizing Ecologically Relevant Variations In Streamflow Regimes, Kiran J. Chinnayakanahalli

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Maintaining the ecological health of streams is vital for sustainable water resources management. Streamflow is a primary factor influencing the structure and function of ecological communities. A quantitative understanding of how stream biota respond to variation in streamflow is required for stream bioassessment. This dissertation focuses on quantifying relationships between streamflow regime and stream macroinvertebrate richness and composition. The contribution comprises statistical models that predict stream macroinvertebrate class from streamflow regime and predict streamflow regime from watershed attributes, and a tool that helps derive watershed attribute variables used in these models.

The dissertation is a collection of three papers. In …

Transesterification And Recovery Of Intracellular Lipids Using A Single Step Reactive Extraction, Daniel R. Nelson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A single-step, extractive reaction for extraction of lipids such as biodiesel components, omega-3 fatty acids, or other triglycerides from microbial cells was examined. Conventional methods for lipid extraction use toxic solvents, and require multiple steps and long processing times. When the goal is to produce fatty acid methyl esters or FAMEs, the extracted lipids are subjected to a separate transesterification reaction with simple alcohols in the presence of an acid or base catalyst. A simplified, single-step reactive extraction method can be applied that combines the sequential extraction followed by transesterification using acidified alcohols - a process known as in situ …

A Synthetic Biological Engineering Approach To Secretion- Based Recovery Of Polyhydroxyalkanoates And Other Cellular Products, Elisabeth Linton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The costs associated with cellular product recovery commonly account for as much as 80% of the total production expense. As a specific example, significant recovery costs limit commercial use of polyhydroxyalkanoates (PHA), which comprise a class of microbially-accumulated polyesters. PHAs are biodegradable compounds that are of interest as a sustainable alternative to petrochemically-derived plastics. Secretion-based recovery of PHAs was studied to decrease PHA production costs. Type I and II secretory pathways are commonly used for the translocation of recombinant proteins out of the cytoplasm of E. coli. Proteins were targeted for translocation using four signal peptides (HlyA, TorA, GeneIII, …

Designing An Instrument Based On Native Fluorescence To Determine Soil Microbial Content At A Mars Analog Site, Heather D. Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

For this research project we designed an instrument to detect bacteria via biomolecular fluorescence. We introduce the current understanding of astrobiology, our knowledge of life beyond Earth, and the commonality of Earth life as it pertains to the search for life on Mars. We proposed a novel technique for searching for direct evidence of life on the surface of Mars using fluorescence. We use the arid region of the Mojave Desert as an analog of Mars. Results indicate the fluorescence of the biotic component of desert soils is approximately as strong as the fluorescence of the mineral component. Fluorescence laboratory …

Inducing A Normal Phenotype In Breast Epithelial Cells Using A Three-Dimensional Basement Membrane Extract Culture System: A Study On The Reversion Of Cancer, Ross H. Booth

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Experimentally, traditional developmental models and transgenic animals consistently underscore the importance of studying cell behavior in the correct tissue context. However, live animal experimentation is inherently complex, and systematic assessment of the effects of individual variables, such as cell shape and matrix compliance on cell behavior, is extremely difficult at best. Two-dimensional monolayer culture of key individual cell types has provided abundant, fundamental information on cell response, but cannot be used to show the normal phenotype of breast epithelial cells. Furthermore, their results often fail to translate into in vivo and clinical studies. It has been previously established that normal …

Dechlorinating And Iron Reducing Bacteria Distribution In A Trichloroethene Contaminated Aquifer, Carmen Lourdes Yupanqui Zaa

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Operable Unit 5 (OU 5) area of Hill Air Force Base currently has two trichloroethene-contaminated groundwater plumes underneath residential areas in Sunset and Clinton, Utah. Bioremediation by biological reductive dechlorination can be an important mechanism for the removal of chlorinated compounds from the plumes. The presence of suitable bacteria to carry on reductive dechlorination is the key in the bioremediation process. The goal of this study was to determine the distribution and population density of the 16S rRNA genes of Bacteria, Dehalococcoides ethenogenes, Desulfuromonas michiganensis, Geobacter spp and Rhodoferax ferrireducens-like bacteria, as well as the functional genes trichloroethene reductive …

Estimating Total Phosphorus And Total Suspended Solids Loads From High Frequency Data, Amber Spackman Jones

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Frequently measured turbidity was examined as a surrogate for total phosphorus (TP) and total suspended solids (TSS) loads at two locations in the Little Bear River, Utah, USA. Using regression techniques, equations were developed for TP and TSS as functions of turbidity. The equations accounted for censored data, and additional explanatory variables to represent hydrological conditions were considered for inclusion in the equations. By using the resulting surrogate relationships with high frequency turbidity measurements, high frequency estimates of TP and TSS concentrations were calculated. To examine the effect of sampling frequency, reference loads were determined from the concentration records for …

Evapotranspiration Using A Satellite-Based Surface Energy Balance With Standardized Ground Control, Ricardo Trezza

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study evaluated the potential of using the Surface Energy Balance Algorithm for Land (SEBAL) as a means for estimating evapotranspiration (ET) for local and regional scales in Southern Idaho. The original SEBAL model was refined during this study to provide better estimation of ET in agricultural areas and to make more reliable estimates of ET from other surfaces as well, including mountainous terrain. The modified version of SEBAL used in this study, termed as SEBAL_ID (lD stands for Idaho) includes standardization of the two SEBAL "anchor" pixels, the use of a water balance …

Spectral Signature Studies For Application In Deer Census Using Remote Sensing Techniques, Maran C. Pate

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was performed to determine the spectral signatures of deer and their natural background elements for censusing purposes. Consideration was given to atmospheric transmittance, acceptable flying weather, and terrain. Possible spectral bands between 0.3 and 14.0 μm were obtained (over a pathlength of 1500 feet at an altitude of 5000 feet) based upon atmospheric transmittance using the LOWTRAN 3B computer program. They are: 0.30 - 1.33, 1.49 - 1.79, 2.00 - 2.50, 3.00 - 3.16, 3.38 - 4.10, 4.59 - 5.05, and 8.00 - 13.33 μm, for transmittance greater than 75%. Weather conditions are favorable for flying and taking …

An Evaluation Of In-Stream Structures Designed To Provide Fish Habitat, Charles H. Call Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In-stream concrete structures were studied through model tests and river tests. The model studies indicated that four designs provided good habitat in the model stream. These structures were the inverted weir, the "V" structure, the slab with legs and the cylinder. Through the river studies it was determined that these structures did not influence enough of the total river area to be effective in providing good fish habitat. Also an appreciable amount of yearly maintenance would be required to free the structures from silting in, debris, and vandalism. The slab with legs was the only promising structure.