Engineering Commons^™

Two Approaches For Cell Retention In Perfusion Culture Systems, Zhaowei Wang

ETD Archive

A lack of efficient, economical, and reliable cell retention devices has limited the application of perfusion culture systems in the biopharmaceutical industry. Two types of cell retention devices were developed in this work for long-term perfusion culture systems: a modification of an inclined gravity settler and a variation of an ultrasonic filter. Both bench-top and large-scale tests showed that the gravity settler can effectively retain viable cells and preferentially remove nonviable cells in perfusion culture systems. The viable cell retention rate can be maintained well above 90 during long-term perfusion culture period while the nonviable cell retention rate is 20-30 …