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Theses/Dissertations

Biomedical Engineering and Bioengineering

Cleveland State University

2009

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Articles 1 - 11 of 11

Full-Text Articles in Engineering

Two Approaches For Cell Retention In Perfusion Culture Systems, Zhaowei Wang Jan 2009

Two Approaches For Cell Retention In Perfusion Culture Systems, Zhaowei Wang

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A lack of efficient, economical, and reliable cell retention devices has limited the application of perfusion culture systems in the biopharmaceutical industry. Two types of cell retention devices were developed in this work for long-term perfusion culture systems: a modification of an inclined gravity settler and a variation of an ultrasonic filter. Both bench-top and large-scale tests showed that the gravity settler can effectively retain viable cells and preferentially remove nonviable cells in perfusion culture systems. The viable cell retention rate can be maintained well above 90 during long-term perfusion culture period while the nonviable cell retention rate is 20-30 ...


Cortical Bone Tissue Engineering;Scaffold Design And Cell Selection, Demin Wen Jan 2009

Cortical Bone Tissue Engineering;Scaffold Design And Cell Selection, Demin Wen

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Cortical bone tissue engineering provides a promising approach to generate graft materials needed to treat the large sized bone defects. The underling premise of tissue engineering is to mimic the in vivo microenvironment as best as possible in vitro culture system. To select an appropriate scaffold material used in this model system, mechanical and hydraulic permeability properties of 316L porous stainless steel and polymethylmethacrylate (PMMA) were studied, as well as their biocompatibility in short and long term cell culture. Results showed that scaffolds made of both stainless steel and PMMA: (1) could be manufactured to have similar permeability as that ...


Flow Measurements In Turbulent Flow Fields With Magnetic Resonance Phase Velocity Mapping, Navneeth Sagar Reddy Lakkadi Jan 2009

Flow Measurements In Turbulent Flow Fields With Magnetic Resonance Phase Velocity Mapping, Navneeth Sagar Reddy Lakkadi

ETD Archive

Arterial stenosis (narrowing) is due to vascular disease that, if untreated, leads to death. The stenosis causes the development of complex flow which results in high energy losses and great forces between blood and tissue. Traditionally, turbulent flows lead to signal loss in magnetic resonance (MR) image acquisitions, resulting in loss of diagnostic information. With the advancement in MR hardware and software, some of the limitations of MR in turbulent flow fields may have been reduced or eliminated. This study evaluated the potential of MR phase velocity mapping (MRPVM), a clinical velocimetry technique, under turbulent flow conditions such as those ...


The Synthesis And Characterization Of Novel Elastin-Like Polypeptides Containing An Oligomerization Domain, James T. Cole Jan 2009

The Synthesis And Characterization Of Novel Elastin-Like Polypeptides Containing An Oligomerization Domain, James T. Cole

ETD Archive

Elastin like polypeptides (ELPs) are an emerging class of biomaterials due to their unique group of chemical, physical and biological properties. We have constructed a library of ELPs based on the pentapeptide amino acid repeat sequence (GVGVP). ELPs exhibit lower critical solution temperature (LCST) transition behavior from a soluble phase below the transition temperature (Tt) to an insoluble two phase system above the transition temperature. This process is completely reversible so that ELPs can become soluble again upon lowering the temperature. This temperature can be tuned in many different ways at the molecular level by changing the composition of the ...


Application Of Dual-Energy Computed Tomography To The Evalution Of Coronary Atherosclerotic Plaque, Mitya M. Barreto Jan 2009

Application Of Dual-Energy Computed Tomography To The Evalution Of Coronary Atherosclerotic Plaque, Mitya M. Barreto

ETD Archive

Atherosclerotic coronary artery disease is responsible for around 50 of cardiovascular deaths in USA. Early detection and characterization of coronary artery atherosclerotic plaque could help prevent cardiac events. Computed tomography (CT) is an excellent modality for imaging calcifications and has higher spatial resolution than other common non-invasive modalities (e.g MRI), making it more suitable for coronary plaque detection. However, attenuation-based classification of non-calcified plaques as fibrous or lipid is difficult with conventional CT, which relies on a single x-ray energy. Dual-energy CT (DECT) may provide additional attenuation data for the identification and discrimination of plaque components. The purpose of ...


Subject Specific Computational Models Of The Knee To Predict Anterior Cruciate Ligament Injury, Bhushan S. Borotikar Jan 2009

Subject Specific Computational Models Of The Knee To Predict Anterior Cruciate Ligament Injury, Bhushan S. Borotikar

ETD Archive

Knee joint is a complex joint involving multiple interactions between cartilage, bone, muscles, ligaments, tendons and neural control. Anterior Cruciate Ligament (ACL) is one ligament in the knee joint that frequently gets injured during various sports or recreational activities. ACL injuries are common in college level and professional athletes especially in females and the injury rate is growing in epidemic proportions despite significant increase in the research focusing on neuromuscular and proprioceptive training programs. Most ACL injuries lead to surgical reconstruction followed by a lengthy rehabilitation program impacting the health and performance of the athlete. Furthermore, the athlete is still ...


Development Of A Focused Broadband Ultrasonic Transducer For High Resolution Fundamental And Harmonic Intravascular Imaging, Chaitanya K. Chandrana Jan 2009

Development Of A Focused Broadband Ultrasonic Transducer For High Resolution Fundamental And Harmonic Intravascular Imaging, Chaitanya K. Chandrana

ETD Archive

Intravascular ultrasound (IVUS) is increasingly employed for detection and evaluation of coronary artery diseases. Tissue Harmonic Imaging provides different tissue information that could additionally be used to improve diagnostic accuracy. However, current IVUS systems, with their unfocussed transducers, may not be capable of operating in harmonic imaging mode. Thus, there is a need to develop suitable transducers and appropriate techniques to allow imaging in multi modes for complementary diagnostic information. Focused PVDF TrFE transducers were developed using MEMS (Micro-Electro-Mechanical-Systems) compatible protocols. The transducers were characterized using pulse-echo techniques and exhibited broad bandwidth (110 at -6dB) with axial resolutions of Such ...


Altered Cortico-Cortical Brain Connectivity During Muscle Fatigue, Zhiguo Jiang Jan 2009

Altered Cortico-Cortical Brain Connectivity During Muscle Fatigue, Zhiguo Jiang

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Traditional brain activation studies using neuroimaging such as functional magnetic imaging (fMRI) have shown that muscle fatigue at submaximal intensity level is associated with increased brain activity in various cortical regions from low- to high-order motor centers. However, how these areas might interact remain unclear since previous activation studies related to motor control could not reveal information of between-area interaction. This issue can be addressed by evaluating brain activation data using the framework of connectivity analysis. Three types of brain connectivity, functional connectivity (FC), effective connectivity (EC) and structural connectivity (SC) have been examined to investigate the effect of voluntary ...


Imaging Of Tyramine-Substituted Hydrogels For Tissue Replacement, Ediuska V. Laurens Jan 2009

Imaging Of Tyramine-Substituted Hydrogels For Tissue Replacement, Ediuska V. Laurens

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Novel tyramine-based hyaluronan (HA) and collagen hydrogels have been developed in which cross-linking is accomplished via peroxidase-mediated dityramine linkages allowing direct cross-linking in vivo. These TB hydrogels possess advantageous physical properties, which include excellent biocompatibility and the ability to mimic the biological, structural and mechanical properties of normal, healthy tissues, including cartilage, and thus provide for synthetic, implantable biomaterials suitable for a wide range of tissue types. The efficacy of these TB-hydrogels has been previously tested in a number of clinically relevant animal models, which have evaluated their applicability for the repair/replacement of various tissues, including cartilage. Nevertheless, there ...


Semi-Automatic Segmentation Of Normal Female Pelvic Floor Structures From Magnetic Resonance Images, Xiaolong Li Jan 2009

Semi-Automatic Segmentation Of Normal Female Pelvic Floor Structures From Magnetic Resonance Images, Xiaolong Li

ETD Archive

Stress urinary incontinence (SUI) and pelvic organ prolapse (POP) are important health issues affecting millions of American women. Investigation of the cause of SUI and POP requires a better understand of the anatomy of female pelvic floor. In addition, pre-surgical planning and individualized treatment plans require development of patient-specific three-dimensional or virtual reality models. The biggest challenge in building those models is to segment pelvic floor structures from magnetic resonance images because of their complex shapes, which make manual segmentation labor-intensive and inaccurate. In this dissertation, a quick and reliable semi-automatic segmentation method based on a shape model is proposed ...


Slurry Based Coatings On Silicon Based Ceramics, Muralidhar Challarapu Jan 2009

Slurry Based Coatings On Silicon Based Ceramics, Muralidhar Challarapu

ETD Archive

Silicon carbide/silicon carbide (SiC/SiC) composites are the leading candidates for advanced high temperature structural components in gas turbine engine application. When SiC surface reacts with O2, protective SiO2 scales are formed, which are the basis for the corrosion resistance of SiC. Their main draw back in terms of usage in a gas-turbine engine is the volatilization of the protective silica scale because of the water vapor which is a byproduct of fuel combustion. The purpose of this project was to develop environmental barrier coatings (EBCs) based on ceramic powders to provide resistance to such moisture damage. In this ...