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Full-Text Articles in Engineering
Roadmap On Biological Pathways For Electronic Nanofabrication And Materials, Reza M. Zadegan
Roadmap On Biological Pathways For Electronic Nanofabrication And Materials, Reza M. Zadegan
Materials Science and Engineering Faculty Publications and Presentations
Conventional microchip fabrication is energy and resource intensive. Thus, the discovery of new manufacturing approaches that reduce these expenditures would be highly beneficial to the semiconductor industry. In comparison, living systems construct complex nanometer-scale structures with high yields and low energy utilization. Combining the capabilities of living systems with synthetic DNA-/protein-based self-assembly may offer intriguing potential for revolutionizing the synthesis of complex sub-10 nm information processing architectures. The successful discovery of new biologically based paradigms would not only help extend the current semiconductor technology roadmap, but also offer additional potential growth areas in biology, medicine, agriculture and sustainability for the …
Availability: A Metric For Nucleic Acid Strand Displacement Systems, Xiaoping Olson, Shohei Kotani, Jennifer E. Padilla, Natalya Hallstrom, Sara Goltry, Jeunghoon Lee, Bernard Yurke, William L. Hughes, Elton Graugnard
Availability: A Metric For Nucleic Acid Strand Displacement Systems, Xiaoping Olson, Shohei Kotani, Jennifer E. Padilla, Natalya Hallstrom, Sara Goltry, Jeunghoon Lee, Bernard Yurke, William L. Hughes, Elton Graugnard
Materials Science and Engineering Faculty Publications and Presentations
DNA strand displacement systems have transformative potential in synthetic biology. While powerful examples have been reported in DNA nanotechnology, such systems are plagued by leakage, which limits network stability, sensitivity, and scalability. An approach to mitigate leakage in DNA nanotechnology, which is applicable to synthetic biology, is to introduce mismatches to complementary fuel sequences at key locations. However, this method overlooks nuances in the secondary structure of the fuel and substrate that impact the leakage reaction kinetics in strand displacement systems. In an effort to quantify the impact of secondary structure on leakage, we introduce the concepts of availability and …
Enhanced Dna Sensing Via Catalytic Aggregation Of Gold Nanoparticles, Herbert M. Huttanus, Elton Graugnard, Bernard Yurke, William B. Knowlton, Wan Kuang, William L. Hughes, Jeunghoon Lee
Enhanced Dna Sensing Via Catalytic Aggregation Of Gold Nanoparticles, Herbert M. Huttanus, Elton Graugnard, Bernard Yurke, William B. Knowlton, Wan Kuang, William L. Hughes, Jeunghoon Lee
Materials Science and Engineering Faculty Publications and Presentations
A catalytic colorimetric detection scheme that incorporates a DNA-based hybridization chain reaction into gold nanoparticles was designed and tested. While direct aggregation forms an inter-particle linkagefrom only one target DNA strand, catalytic aggregation forms multiple linkages from a single target DNA strand. Gold nanoparticles were functionalized with thiol-modified DNA strands capable of undergoing hybridization chain reactions. The changes in their absorption spectra were measured at different times and target concentrations and compared against direct aggregation. Catalytic aggregation showed a multifold increase in sensitivity at low target concentrations when compared to direct aggregation. Gelelectrophoresis was performed to compare DNA hybridization reactions …
Programmable Periodicity Of Quantum Dot Arrays With Dna Origami Nanotubes, Hieu Bui, Craig Onodera, Carson Kidwell, Yerpeng Tan, Elton Graugnard, Wan Kuang, Jeunghoon Lee, William B. Knowlton, Bernard Yurke, William L. Hughes
Programmable Periodicity Of Quantum Dot Arrays With Dna Origami Nanotubes, Hieu Bui, Craig Onodera, Carson Kidwell, Yerpeng Tan, Elton Graugnard, Wan Kuang, Jeunghoon Lee, William B. Knowlton, Bernard Yurke, William L. Hughes
Materials Science and Engineering Faculty Publications and Presentations
To fabricate quantum dot arrays with programmable periodicity, functionalized DNA origami nanotubes were developed. Selected DNA staple strands were biotin-labeled to form periodic binding sites for streptavidin-conjugated quantum dots. Successful formation of arrays with periods of 43 and 71 nm demonstrates precise, programmable, large-scale nanoparticle patterning; however, limitations in array periodicity were also observed. Statistical analysis of AFM images revealed evidence for steric hindrance or site bridging that limited the minimum array periodicity.