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Evaluation Of Swine-Specific Pcr Assays Used For Fecal Source Tracking And Analysis Of Molecular Diversity Of Swine-Specific "Bacteroidales" Populations, Regina Lamendella, Jorge W. Santo Domingo, Anthony C. Yannarell, Shreya Ghosh, Giovanni George Di, Roderick Ian Mackie, Daniel B. Oerther
Evaluation Of Swine-Specific Pcr Assays Used For Fecal Source Tracking And Analysis Of Molecular Diversity Of Swine-Specific "Bacteroidales" Populations, Regina Lamendella, Jorge W. Santo Domingo, Anthony C. Yannarell, Shreya Ghosh, Giovanni George Di, Roderick Ian Mackie, Daniel B. Oerther
Civil, Architectural and Environmental Engineering Faculty Research & Creative Works
In this study, we evaluated the specificity, distribution, and sensitivity of Prevotella strain-based (PF163 and PigBac1) and methanogen-based (P23-2) PCR assays proposed to detect swine fecal pollution in environmental waters. The assays were tested against 222 fecal DNA extracts derived from target and nontarget animal hosts and against 34 groundwater and 15 surface water samples from five different sites. We also investigated the phylogenetic diversity of 1,340 "Bacteroidales" 16S rRNA gene sequences derived from swine feces, swine waste lagoons, swine manure pits, and waters adjacent to swine operations. Most swine fecal samples were positive for the host-specific Prevotella-based PCR assays …