Open Access. Powered by Scholars. Published by Universities.®

Engineering Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 5 of 5

Full-Text Articles in Engineering

3d-Printed Biomimetic Bioactive Glass Scaffolds For Bone Regeneration In Rat Calvarial Defects, Krishna C. R. Kolan, Yue-Wern Huang, Julie A. Semon, Ming-Chuan Leu Apr 2020

3d-Printed Biomimetic Bioactive Glass Scaffolds For Bone Regeneration In Rat Calvarial Defects, Krishna C. R. Kolan, Yue-Wern Huang, Julie A. Semon, Ming-Chuan Leu

Biological Sciences Faculty Research & Creative Works

The pore geometry of scaffold intended for the use in the bone repair or replacement is one of the most important parameters in bone tissue engineering. It affects not only the mechanical properties of the scaffold but also the amount of bone regeneration after implantation. Scaffolds with five different architectures (cubic, spherical, x, gyroid, and diamond) at different porosities were fabricated with bioactive borate glass using the selective laser sintering (SLS) process. The compressive strength of scaffolds with porosities ranging from 60% to 30% varied from 1.7 to 15.5 MPa. The scaffold's compressive strength decreased significantly (up ...


Bioprinting With Human Stem Cell-Laden Alginate-Gelatin Bioink And Bioactive Glass For Tissue Engineering, Krishna C. R. Kolan, Julie A. Semon, Bradley Bromet, D. E. Day, Ming-Chuan Leu Jul 2019

Bioprinting With Human Stem Cell-Laden Alginate-Gelatin Bioink And Bioactive Glass For Tissue Engineering, Krishna C. R. Kolan, Julie A. Semon, Bradley Bromet, D. E. Day, Ming-Chuan Leu

Biological Sciences Faculty Research & Creative Works

Three-dimensional (3D) bioprinting technologies have shown great potential in the fabrication of 3D models for different human tissues. Stem cells are an attractive cell source in tissue engineering as they can be directed by material and environmental cues to differentiate into multiple cell types for tissue repair and regeneration. In this study, we investigate the viability of human adipose-derived mesenchymal stem cells (ASCs) in alginate-gelatin (Alg-Gel) hydrogel bioprinted with or without bioactive glass. Highly angiogenic borate bioactive glass (13-93B3) in 50 wt% is added to polycaprolactone (PCL) to fabricate scaffolds using a solvent-based extrusion 3D bioprinting technique. The fabricated scaffolds ...


Near-Field Electrospinning Of A Polymer/Bioactive Glass Composite To Fabricate 3d Biomimetic Structures, Krishna C. R. Kolan, Jie Li, Sonya Roberts, Julie A. Semon, Jonghyun Park, D. E. Day, Ming-Chuan Leu Jan 2019

Near-Field Electrospinning Of A Polymer/Bioactive Glass Composite To Fabricate 3d Biomimetic Structures, Krishna C. R. Kolan, Jie Li, Sonya Roberts, Julie A. Semon, Jonghyun Park, D. E. Day, Ming-Chuan Leu

Biological Sciences Faculty Research & Creative Works

Bioactive glasses have recently gained attention in tissue engineering and three-dimensional (3D) bioprinting because of their ability to enhance angiogenesis. Some challenges for developing biological tissues with bioactive glasses include incorporation of glass particles and achieving a 3D architecture mimicking natural tissues. In this study, we investigate the fabrication of scaffolds with a polymer/bioactive glass composite using near-field electrospinning (NFES). An overall controlled 3D scaffold with pores, containing random fibers, is created and aimed to provide superior cell proliferation. Highly angiogenic borate bioactive glass (13-93B3) in 20 wt.% is added to polycaprolactone (PCL) to fabricate scaffolds using the NFES ...


Solvent Based 3d Printing Of Biopolymer/Bioactive Glass Composite And Hydrogel For Tissue Engineering Applications, Krishna Kolan, Yong Liu, Jakeb Baldridge, Caroline Murphy, Julie A. Semon, D. E. Day, Ming-Chuan Leu Jul 2017

Solvent Based 3d Printing Of Biopolymer/Bioactive Glass Composite And Hydrogel For Tissue Engineering Applications, Krishna Kolan, Yong Liu, Jakeb Baldridge, Caroline Murphy, Julie A. Semon, D. E. Day, Ming-Chuan Leu

Biological Sciences Faculty Research & Creative Works

Three-dimensional (3D) bioprinting is an emerging technology in which scaffolding materials and cell-laden hydrogels may be deposited in a pre-determined fashion to create 3D porous constructs. A major challenge in 3D bioprinting is the slow degradation of melt deposited biopolymer. In this paper, we describe a new method for printing poly-caprolactone (PCL)/bioactive borate glass composite as a scaffolding material and Pluronic F127 hydrogel as a cell suspension medium. Bioactive borate glass was added to a mixture of PCL and organic solvent to make an extrudable paste using one syringe while hydrogel was extruded and deposited in between the PCL ...


3d Bioprinting Of Stem Cells And Polymer/Bioactive Glass Composite Scaffolds For Bone Tissue Engineering, Caroline Murphy, Krishna Kolan, Wenbin Li, Julie A. Semon, D. E. Day, Ming-Chuan Leu Jan 2017

3d Bioprinting Of Stem Cells And Polymer/Bioactive Glass Composite Scaffolds For Bone Tissue Engineering, Caroline Murphy, Krishna Kolan, Wenbin Li, Julie A. Semon, D. E. Day, Ming-Chuan Leu

Biological Sciences Faculty Research & Creative Works

A major limitation of using synthetic scaffolds in tissue engineering applications is insufficient angiogenesis in scaffold interior. Bioactive borate glasses have been shown to promote angiogenesis. There is a need to investigate the biofabrication of polymer composites by incorporating borate glass to increase the angiogenic capacity of the fabri-cated scaffolds. In this study, we investigated the bioprinting of human adipose stem cells (ASCs) with a polycaprolac-tone (PCL)/bioactive borate glass composite. Borate glass at the concentration of 10 to 50 weight %, was added to a mixture of PCL and organic solvent to make an extrudable paste. ASCs suspended in Matrigel ...