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Use Of Ion Exchange Membranes For Selective Recovery Of Aspergillus Awamori Glucoamylase And Phage T4 Lysozyme, Deborah K. Stafslein, Jeff M. Hunter, Craig E. Forney
Use Of Ion Exchange Membranes For Selective Recovery Of Aspergillus Awamori Glucoamylase And Phage T4 Lysozyme, Deborah K. Stafslein, Jeff M. Hunter, Craig E. Forney
Journal of the Iowa Academy of Science: JIAS
Our research examined whether enhanced separation and purification of mutant enzymes could be obtained on ion-exchange membranes. Solutions of three mutants of Aspergillus awamori glucoamylase were passed through an anionic exchange membrane, as well as one mutant of T4 lysozyme through a cationic exchange membrane. The mutant enzymes were modified by adding "charged fusions'', polypeptides of either aspartic acid residues to increase the overall negative charge of the enzyme or arginine residues to increase the overall positive charge. The effect of the mutations on the purification of glucoamylase from a "modified" fermentation broth were examined at two different elution pHs, …
Selective Recovery Of Β-Galactosidase With Charged Fusion Tails Using Ion-Exchange Membranes, Erika A. Thiem, Meng H. Heng
Selective Recovery Of Β-Galactosidase With Charged Fusion Tails Using Ion-Exchange Membranes, Erika A. Thiem, Meng H. Heng
Journal of the Iowa Academy of Science: JIAS
We explored the feasibility of attaching charged tails to a target protein, in this case β-galactosidase, for selective recovery. In this process, an ion-exchange membrane was used for selective binding and release of β-galactosidase with the attached purification fusions. Strength of binding and purity of eluate increased with increasing tail length. In addition, activity yield was improved with the implementation of an intermediate partial elution recycle procedure.