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1991

Journal of the Iowa Academy of Science: JIAS

Characteristics and purification; GA biosynthesis and metabolism; steroid receptors; nuclei

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In Search Of A Gibberellin Receptor, Lalit M. Srivastava, Konrad A. Sechley Jan 1991

In Search Of A Gibberellin Receptor, Lalit M. Srivastava, Konrad A. Sechley

Journal of the Iowa Academy of Science: JIAS

Use of tritiated gibberellins ([3H] GA1, [3H] GA4) of high specific activity and purity has allowed the determination of GA-specific binding sites in several plant tissues both in vivo and in vitro. In cucumber hypocotyls and pea epicotyls which have been most investigated the binding of [3H] GA; occurs to soluble proteins. This binding is saturable ('n' =about 30 pmol. Mg-1 soluble protein), of high affinity (KD= about 70 nM), and is competed for by other GA, and derivatives in proportion to their biological activity in these tissues in vivo. Size exclusion and ...