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Exacerbation Of Tgf-Β-Induced Cataract By Fgf-2 In Cultured Rat Lenses, Anna Cerra, Kylie Mansfield, Coral Chamberlain
Exacerbation Of Tgf-Β-Induced Cataract By Fgf-2 In Cultured Rat Lenses, Anna Cerra, Kylie Mansfield, Coral Chamberlain
Faculty of Social Sciences - Papers (Archive)
Purpose: Culturing rat lenses with transforming growth factor-beta (TGFβ) results in the formation of anterior, opaque subcapsular plaques which exhibit many of the features of human subcapsular cataract. The present study was undertaken to determine whether this process is influenced by the presence of fibroblast growth factor (FGF), a normal component of the lens environment in situ.
Methods: Rat lenses were cultured for 4-8 days with TGFβ-2, alone or in combination with FGF-2, PDGF-AA, or the growth factor inhibitors poly(4-styrenesulfonic acid) (PSS) and suramin. Responses were assessed by monitoring opacification, by routine histology and immunolocalization of markers for fibrotic change …