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Full-Text Articles in Education
Pkc Inhibition Increases Gap Junction Intercellular Communication And Cell Adhesion In Human Neuroblastoma, M. Morley, C. Jones, M. Sidhu, V. Gupta, S. Bernier, W. Rushlow, Daniel Belliveau
Pkc Inhibition Increases Gap Junction Intercellular Communication And Cell Adhesion In Human Neuroblastoma, M. Morley, C. Jones, M. Sidhu, V. Gupta, S. Bernier, W. Rushlow, Daniel Belliveau
Daniel J. Belliveau
Abstract Gap junction intercellular communication and cell–cell adhesion are essential for maintaining a normal cellular phenotype, including the control of growth and proliferation. Loss of either cell–cell adhesion or communication is common in cancers, while restoration of function is associated with tumor suppression. Protein kinase C (PKC) isozymes regulate a broad spectrum of cellular functions including growth and proliferation, and their overexpression has been correlated with carcinogenesis. Consequently, PKC inhibitors are currently undergoing clinical trials as an anti-cancer agents although the precise cellular alterations induced by PKC inhibitors remain to be elucidated. In the current study, the effects of PKC …
Viral Shedding And Biodistribution Of G207, A Multimutated, Conditionally Replicating Herpes Simplex Virus Type 1, After Intracerebral Inoculation In Aotus, T. Todo, F. Feigenbaum, S. Rabkin, F. Lakeman, J. Newsome, P. Johnson, E. Mitchell, Daniel Belliveau, J. Ostrove, R. Martuza
Viral Shedding And Biodistribution Of G207, A Multimutated, Conditionally Replicating Herpes Simplex Virus Type 1, After Intracerebral Inoculation In Aotus, T. Todo, F. Feigenbaum, S. Rabkin, F. Lakeman, J. Newsome, P. Johnson, E. Mitchell, Daniel Belliveau, J. Ostrove, R. Martuza
Daniel J. Belliveau
G207 is a multimutated, conditionally replicating herpes simplex virus type 1 (HSV-1) that is currently in clinical trial for patients with malignant glioma. G207 exhibits an efficient oncolytic activity in tumor cells, yet minimal toxicity in normal tissue when injected into the brains of HSV-susceptible mice or nonhuman primates. In this study, we evaluated the shedding and biodistribution of clinical-grade G207 after intracerebral inoculation (3 × 107 pfu) in four New World owl monkeys (Aotus nancymae). Using PCR analyses and viral cultures, neither infectious virus nor viral DNA was detected from tear, saliva, or vaginal secretion samples at any time …
Transgenic Mice Expressing The Intracellular Domain Of The P75 Neurotrophin Receptor Undergo Neuronal Apoptosis, M. Majdan, C. Lachance, A. Gloster, R. Aloyz, C. Zeindler, S. Bamji, A. Bhakar, Daniel Belliveau, J. Fawcett, F. Miller, P. Barker
Transgenic Mice Expressing The Intracellular Domain Of The P75 Neurotrophin Receptor Undergo Neuronal Apoptosis, M. Majdan, C. Lachance, A. Gloster, R. Aloyz, C. Zeindler, S. Bamji, A. Bhakar, Daniel Belliveau, J. Fawcett, F. Miller, P. Barker
Daniel J. Belliveau
We have asked whether p75NTR may play a role in neuronal apoptosis by producing transgenic mice that express the p75NTR intracellular domain within peripheral and central neurons. These animals showed profound reductions in numbers of sympathetic and peripheral sensory neurons as well as cell loss in the neocortex, where there is normally little or no p75NTR expression. Developmental loss of facial motor neurons was not observed, but induced expression of the p75NTR intracellular domain within adult animals led to increased motor neuron death after axotomy. Biochemical analyses suggest that these effects were not attributable to a p75NTR-dependent reduction in trk …
In Vivo Growth Of C6 Glioma Cells Transfected With Connexin43 Cdna, C. Naus, K. Elisevich, D. Zhu, Daniel Belliveau, R. Del Maestro
In Vivo Growth Of C6 Glioma Cells Transfected With Connexin43 Cdna, C. Naus, K. Elisevich, D. Zhu, Daniel Belliveau, R. Del Maestro
Daniel J. Belliveau
In order to examine the possible role of intercellular communication via gap junctions in the control of tumor growth, we have transfected C6 glioma cells with connexin43 cDNA. We obtained several clones with variable expression of connexin43. The growth rate of these clones in culture was inversely related to the degree of expression of the transfected cDNA. To examine the growth of these transfected cells in vivo, cells were grown in spinner culture flasks to form spheroids 250–300 µm in diameter. Spheroids of nontransfected C6 cells produced large gliomas. Immunohistochemical and in situ hybridization analyses revealed relatively high levels of …