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Articles 1 - 3 of 3
Full-Text Articles in Arts and Humanities
Mapping Adenines, Guanines, And Pyrimidines In Rna, Helen Donis-Keller, Allan Maxam, Walter Gilbert
Mapping Adenines, Guanines, And Pyrimidines In Rna, Helen Donis-Keller, Allan Maxam, Walter Gilbert
Helen Donis-Keller
The positions of adenines, guanines, and pyrimidines can be determined by partial nuclease digestion of a terminally labeled RNA molecule. In urea, at elevated temperatures, RNase T1 generates a pattern reflecting cleavage at guanines while RNase U2 cleaves only at adenine. A limited alkaline hydrolysis provides a continuum of fragments derived from breaks at every phosphodiester bond. The reaction products are electrophoretically fractionated by size in adjacent lanes of a polyacrylamide gel. An autoradiograph of the gel displays the sequence up to 100 nucleotides from the end of the molecule, although uracil cannot as yet be distinguished from cytosine. These …
The Decline Of History, Rowan Cahill
The Decline Of History, Rowan Cahill
Rowan Cahill
Written at a time when the teaching of 'History' was declining in Australian secondary schools (1970s), this is a view from the classroom by a classroom teacher. The author trenchantly defends the place of 'History' as a subject in Secondary schools, and opposes its teaching by non-history trained teachers, as well as the introduction of 'thematic' approaches. Instead he defends a broad 'History' curriculum, the exploration of cause and effect, and for Senior students, their introduction to the notion of 'historiography'.
The Decline Of History, Rowan Cahill
The Decline Of History, Rowan Cahill
Rowan Cahill
The author addresses the contemporary (1970s) loss of confidence, and interest, in history as a subject amongst Australian secondary school students and educational administrators. He mounts a defence of the teaching of the subject in schools, and argues for its complexities. Strategies to increase the appeal of the subject and its perceived relevance are suggested.