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Effect Of Histatin- And Statherin-Derived Engineered Salivary Peptides On Streptococcus Mutans Adhesion And On Enamel Demineralization Provoked By Cariogenic Biofilms, Lina Maria Marin
Electronic Thesis and Dissertation Repository
Dental biofilm is formed onto dental surfaces covered by a layer of specific salivary proteins and peptides named acquired enamel pellicle (AEP). It was previously demonstrated that the statherin- and histatin-derived engineered salivary peptides DR9-DR9 and DR9-RR14 were able to reduce enamel demineralization and displayed antimicrobial activity against Streptococcus mutans, respectively. However, these studies were carried under experimental conditions that did not mimic caries development induced by biofilm and sucrose exposure, the most cariogenic dietary sugar. In this thesis we assessed the effect of the engineered salivary peptides on 1) the adherence of S. mutans to hydroxyapatite (HAp), and …
Mass Spectrometry-Based Proteomics Analysis Of Bioactive Proteins In Emd That Modulate Adhesion Of Gingival Fibroblast To Improve Bio-Integration Of Dental Implants, David Zuanazzi Machado Jr
Mass Spectrometry-Based Proteomics Analysis Of Bioactive Proteins In Emd That Modulate Adhesion Of Gingival Fibroblast To Improve Bio-Integration Of Dental Implants, David Zuanazzi Machado Jr
Electronic Thesis and Dissertation Repository
Titanium implants are used in dental practice to replace damaged or lost teeth. The implant needs to integrate with the surrounding gingiva to protect it against bacterial invasion that leads to implant loss. The biointegration is dependent on the implant surface that interacts with proteins from biological fluids to modulate tissues response. Tailoring the surface with specific proteins from the enamel matrix derivative (EMD) would be beneficial to improve the implant-gingiva interface since EMD can affect various cells including gingival fibroblasts. A surface-affinity approach using three different titanium surfaces and saliva was utilized as a model in combination with tandem …
Identification Of The Salivary Proteome In Children Throughout The Course Of Dental Eruption, Nada Tabbara
Identification Of The Salivary Proteome In Children Throughout The Course Of Dental Eruption, Nada Tabbara
Electronic Thesis and Dissertation Repository
The salivary proteome is recognized as a valuable source of potential oral and systemic disease biomarkers. Major efforts in salivary research have been dedicated to identify and characterize salivary proteins present in saliva using both classical biochemical methods and proteomics approaches in adults. Despite considerable research on the salivary proteome, little attention has been given to the changes in the salivary proteome occurring in children, specifically from 0-3 years of age. Through the use of anionic PAGE, SDS PAGE, HPLC and MS/MS, salivary protein profiles in children before, during and after dental eruption were compared with edentulous adult controls. We …