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Articles 2942611 - 2942640 of 4735627
Full-Text Articles in Entire DC Network
Eng 1002c-010-031-050: Composition And Literature, Debra Valentino
Eng 1002c-010-031-050: Composition And Literature, Debra Valentino
Spring 2000
No abstract provided.
Eng 1000-001: Fundamental English, Denise Fitzer, Julie Perenchio
Eng 1000-001: Fundamental English, Denise Fitzer, Julie Perenchio
Spring 2000
No abstract provided.
Eng 4760-001: Writing For The Professions, Jerie Weasmer
Eng 4760-001: Writing For The Professions, Jerie Weasmer
Spring 2000
No abstract provided.
Eng 5006-001: Recent British Drama, David Radavich
Eng 1002c-036: Composition And Literature, Spear
Eng 1002c-034: Composition And Literature, Michael Kuo
Eng 1002c-034: Composition And Literature, Michael Kuo
Spring 2000
No abstract provided.
Eng 1002c-051-060: Composition And Literature, Robert Zordani
Eng 1002c-051-060: Composition And Literature, Robert Zordani
Spring 2000
No abstract provided.
Eng 3001-004: Advanced Composition, Mcdonough
Eng 2007-001, John Kilgore
Eng 3009c-011: Myth And Culture, Jyoti Panjwani
Eng 2205-003: Introduction To Literary Studies, Stephen Swords
Eng 2205-003: Introduction To Literary Studies, Stephen Swords
Spring 2000
No abstract provided.
Eng 1002c-023-043-055: Composition And Literature, Jay Prefontaine
Eng 1002c-023-043-055: Composition And Literature, Jay Prefontaine
Spring 2000
No abstract provided.
Eng 3009c-004: Myth And Culture, Carol Stevens
Eng 1092c-096: Composition And Literature, Mary Maddox
Eng 1092c-096: Composition And Literature, Mary Maddox
Spring 2000
No abstract provided.
Eng 3001-003: Advanced Composition, Gregg Hecimovich
Eng 3001-003: Advanced Composition, Gregg Hecimovich
Spring 2000
No abstract provided.
Eng 1092c-098, Janelle Carey
Eng 3010c-002: Literary Masterworks, John Allison
Eng 2009c-001, Linda Coleman
Eng 3001-006: Advanced Composition, Jerie Weasmer
Eng 3701-002: American Romanticism, P A. Boswell
Eng 3009c-002-009: Myth And Culture, William Miller
Eng 4762-001: Creative Writing: Poetry, Guzlowski
Eng 4850-115: Literature Of Vietnam, Martone
Eng 3604-001: Special Topics (The Literature Of The City: New York, London, Paris), Richard Sylvia
Eng 3604-001: Special Topics (The Literature Of The City: New York, London, Paris), Richard Sylvia
Spring 2000
No abstract provided.
Eng 3703-002-115, W D. Miller
Eng 3808-001: 20th-Century British Literature, Michael Leddy
Eng 3808-001: 20th-Century British Literature, Michael Leddy
Spring 2000
No abstract provided.
Eng 3901-001: Language And Linguistics, Rosemary Buck
Eng 3901-001: Language And Linguistics, Rosemary Buck
Spring 2000
No abstract provided.
Eng 3808-002, Christhilf
Use Of Biotin-Labeled Nucleic Acids For Protein Purification And Agarose-Based Chemiluminescent Electromobility Shift Assays., Joseph Rodgers, Pinky Patel, Jason Hennes, Sara Bolognia, David Mascotti
Use Of Biotin-Labeled Nucleic Acids For Protein Purification And Agarose-Based Chemiluminescent Electromobility Shift Assays., Joseph Rodgers, Pinky Patel, Jason Hennes, Sara Bolognia, David Mascotti
David P. Mascotti
We have employed biotin-labeled RNA to serve two functions. In one, the biotin tethers the RNA to streptavidin–agarose beads, creating an affinity resin for protein purification. In the other, the biotin functions as a label for use in a modified chemiluminescent electromobility shift assay (EMSA), a technique used to detect the formation of protein–RNA complexes. The EMSA that we describe avoids the use not only of radioactivity but also of neurotoxic acrylamide by using agarose as the gel matrix in which the free nucleic acid is separated from protein–nucleic acid complexes. After separation of free from complexed RNA in agarose, …
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