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Structural Analysis Of Cytokine Signaling Modulators, Cristina Ileana Silvescu
Structural Analysis Of Cytokine Signaling Modulators, Cristina Ileana Silvescu
Doctoral Dissertations
The N-linked glycans attached to cytokine receptors modulate signal transduction by interacting with galectin-3 and generating a cell surface lattice. This opposes constitutive endocytosis and reduces the thresholds for cytokine signaling. The preferred ligands for galectin-3 are the poly-N-acetyl lactosaminylated tetra-antennary glycans which are synthesized by Mgat 5 and are present at high levels in tumor cells. A null mutation in Mgat 5 inhibits lattice formation and cancer progression in cells with an oncogenic background. GlcNAc feeding reestablishes lattice formation, cytokine signaling and tumorigenesis.
A MALDI-TOF analysis was employed to identify the N-glycans involved in modulating signal transduction in wild …
Regulation Of Rod Photoreceptor Phosphodiesterase (Pde6) By The Glutamic Acid -Rich Protein 2 (Garp2), Dana C. Pentia
Regulation Of Rod Photoreceptor Phosphodiesterase (Pde6) By The Glutamic Acid -Rich Protein 2 (Garp2), Dana C. Pentia
Doctoral Dissertations
Rod photoreceptor phosphodiesterase (PDE6), the central enzyme of visual transduction in vertebrate photoreceptors, associates with the disk membranes of the rod outer segment (ROS) of the photoreceptor cell. This association insures the high efficiency of activation by the G-protein, transducin, and the precise control of its inactivation. In addition to binding to transducin during visual excitation, PDE6 is hypothesized to be regulated by other interacting proteins.
The first aim of this research was to isolate and identify the proteins that interact with PDE6 during various stages of the visual signaling pathway. We evaluated methods for solubilizing PDE6 and its binding …
Analysis Of The Role Of Poly(A) -Binding Protein (Pab1) In The Mrna Degradation Process In Yeast, Gang Yao
Analysis Of The Role Of Poly(A) -Binding Protein (Pab1) In The Mrna Degradation Process In Yeast, Gang Yao
Doctoral Dissertations
The mRNA deadenylation process influences multiple aspects of protein synthesis and is known to be the major factor controlling mRNA decay rates. My data demonstrates that yeast PAB1 plays both positive and negative roles in controlling deadenylation, and I have identified particular regions of PAB1 involved in controlling different aspects of the mRNA degradative process. I have found that yeast PAB1 does not play a simple, obstructionist role in regulating CCR4 deadenylation. Instead, PAB1-PAB1 protein interactions, as mediated by the PAB1 proline-rich region (P domain) and the RRM1 domain, are required for the CCR4 deadenylase activity. The P and RRM1 …