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Microvascular Endothelial Cells Of The Bovine Corpus Luteum: A Comparative Study Of The Estrous Cycle And Pregnancy, Jessica A. Cherry

Master's Theses and Capstones

Endothelial cells derived from the corpus luteum (CLENDOs) exhibit a diverse array of characteristics that seemingly complement their wide-ranging properties in luteal function and fate. Reproductive status of the animal (i.e., non-pregnant vs. pregnant) may contribute to the phenotypic/physiologic diversity of microvascular endothelial cells, but information in this regard is limited. Here, structural and functional attributes of CLENDOs derived from the estrous cycle (Days 9-12) were compared with those of pregnancy (Day 60). Initially, lectin binding properties were examined in the cells. Using fluorescent-tagged lectins for Bandeiraea simplicifolia I or concanavalin A, flow cytometric analysis indicated that lectin-binding on CLENDOs …

The Expression Of Heat Shock Protein 70, Heat Shock Protein 90 And Heat Shock Factor 1 Within The Bovine Midcycle Corpus Luteum After Stimulation With Temperature In Vitro And Pgf2alpha In Vitro And In Vivo, Melissa N. Kopka

Master's Theses and Capstones

Environmental and biological stressors affect cellular function. Heat shock proteins (HSPs) are molecular chaperones that safeguard cells against these stressors by protecting endogenous proteins from degradation. Heat shock factors (HSF) are their transcriptional regulators. In the present study, we evaluated whether heat (Experiment 1) or prostaglandin F2alpha (PGF2alpha; Experiments 2 and 3) induces the expression of HSP 70, HSP 90 and HSF1 in the bovine corpus luteum (CL). In summary, in vitro heat treatment elicited a later response in luteal cells by increasing HSP 70 expression at 24 hrs, while in vivo PGF2alpha treatment leads to an earlier response by …

The Effects Of Photoperiod And Water Temperature On The Reproductive Development Of Black Sea Bass, Centropristis Striata, Katie Strait

Master's Theses and Capstones

Black sea bass are a potential candidate for marine aquaculture due to their high market value, high demand, and limited seasonal supply. One of the most significant impediments to commercial production of black sea bass is the lack of a year-round supply of eggs and larvae. Further, control of reproductive development is poorly understood in this species. Black sea bass are protogynous hermaphrodites that change sex between 2-5 years of age. It is unclear why adult black sea bass undergo sex change in the wild. Therefore, the first part of study sought to determine if density is involved in sex …

Temporal Expression Of Protein Mediators During Pgf2alpha-Induced Luteolysis In Sheep, Bryon F. Ricketts

Master's Theses and Capstones

To study dynamic in vivo biochemical changes within the corpus luteum (CL) during luteolysis, a physiological model mimicking the onset of natural luteolysis was established, in which sheep received sequential systemic pulses of PGF2alpha (20ug/min/lhr) at mid-luteal phase of the estrous cycle. We previously demonstrated that after one pulse of PGF2alpha, the protein levels of extracellular matrix (ECM) regulators, tissue inhibitors of metalloproteinases (TIMPs) -1 and -2 decreased dramatically within one hour of PGF2alpha infusion, while matrix metalloproteinase (MMP)-2 activity increased 4 hrs post infusion. In summary, the early and dramatic decrease in TIMP-1 and TIMP-2 proteins, accompanied by an …

Patterns And Processes Of Soil Carbon Dynamics In A Northeastern United States Forest, Sarah K. Silverberg

Master's Theses and Capstones

Forest soils represent a substantial component of the terrestrial carbon cycle and are an important research area for a number of carbon cycle science initiatives. Whereas patterns of aboveground productivity have been relatively well measured and are increasingly included in regional-scale model analyses, belowground estimates are still highly uncertain and progress has been hampered by methodological difficulties. The lack of data poses a problem because belowground measurements are needed to create complete carbon budgets for terrestrial ecosystems at local, regional and global scales. Ecosystem carbon balances will help identify how and where carbon is being stored, as well as how …

Characteristics And Dynamics Of A Moose Population In Northern New Hampshire, Anthony Richard Musante

Master's Theses and Capstones

Abundance indices suggest that the moose (Alces alces) population in northern New Hampshire has stabilized despite favorable habitat and conservative harvest. Natural mortality of unknown cause is presumed a primary reason although little is known about moose reproduction and survival in New Hampshire. This study (2002-2005) was designed to investigate the dynamics that impact this population.

Analysis of harvest reproductive data (1988-2004) indicated that average field-dressed weight of adult cows increased although corpora lutea count declined from ∼1.4- 1.2/cow in the study area and statewide. Yearling ovulation rate (∼42%) and average weight (<211 kg) declined about 25% and 4%, respectively. Parturition of radio-marked cows ranged from 8 May-13 July (median=19 May) with 78% of births from 13-27 May. Calving rate of yearlings and adults (>2 yr) averaged 30 and 85%, respectively, …

Assessing The Accuracy Of The Modis Lai 1-Km Product In Southeastern United States Loblolly Pine Plantations: Accounting For Measurement Variance From Ground To Satellite, John Shepherd Iiames Jr.

Doctoral Dissertations

Leaf area index (LAI), defined here as one-half of the total leaf area per unit ground surface area (Chen, 1996), has been estimated at a global scale from spectral data processed from the Moderate Resolution Imaging Spectroradiometer (MODIS) sensor aboard two NASA EOS-AM spacecraft, Terra (launched in 1999) and Aqua (launched in 2002). The MOD15A2 LAI product is a 1 km global data product composited over an 8-day period and is derived from a three-dimensional radiative transfer model driven by an atmosphere corrected surface reflectance product (MOD09), a land cover product (MOD12) and ancillary information on surface characteristics. The United …

Gonadal Histology And Reproductive Steroidogenesis Of Anurans Exposed To Potential Endocrine Disrupting Contaminants, Emily May La Fiandra

Doctoral Dissertations

The prevalence of malformed amphibians and the association of high incidences of malformation with the presence of environmental contaminants have raised questions about the effects of environmental contaminants on the development of larval amphibians. The potential endocrine disrupting effects of pesticides suggest that the reproductive and developmental abnormalities observed in larval anurans may be due at least in part to impacts on the hypothalamic-pituitary-gonadal axis. The present studies investigate the effects of exposure to environmental contaminants including DDT and atrazine on the development, gonadal histology and reproductive steroidogenesis of larval anurans.

As previous monitoring at Great Bay National Wildlife Refuge …

Patterns And Process During The Diversification Of The Cichlid Fishes In Lake Malawi, Africa, Michael R. Kidd

Doctoral Dissertations

The 500-1000 cichlid species endemic to Lake Malawi constitute one of the most rapid and extensive radiations of vertebrates ever discovered. The objective of this dissertation was to test the assumptions and predictions of several recently published models of cichlid speciation. First, a novel assay of female choice was used to evaluate the role of visual cues during conspecific recognition. The results of this experiment demonstrate that females are able to identify conspecific mates using only visual cues. Second, the assumptions of a recent model of speciation via intrasexual selection were tested by comparing aggressive behavior during territorial contests among …

The Development And Utilization Of Est (Expressed Sequence Tag) Resources In The Diploid Strawberry Model System, Robin Leigh Brese

Master's Theses and Capstones

Enhancement of genomic resources is needed for the cultivated octoploid strawberry Fragaria x ananassa (2n=8x=56). A diploid (2n=2x=14) relative, Fragaria vesca, has been used as a model system for strawberry genomics because of its small (~200 Mb) basic genome size, ancestry to the octoploid strawberries and availability of inbred lines, among other favorable features.

In this study, a cDNA library was constructed from developing flower buds of Fragaria vesca ssp. vesca cv. 'Yellow Wonder'. A cDNA library of 7680 clones was created using the CLONTECH SMART cDNA Library Construction kit. Sequencing and bioinformatic analyses of 3298 clones resulted in 2717 …

Studies Of Flower Color In Anagallis Monelli L, Andrea Quintana Pinilla

Master's Theses and Capstones

One of the main goals for plant breeders in the floriculture industry is to develop plants with new attributes. Characteristics such as flower size, growth habit, resistance to pests and diseases, flower size, and particularly flower color are among the most important traits to breed and improve. One of the best ways to make a plant popular in the market is to offer a wide variety of flower colors. A. monelli is an ornamental plant used for hanging baskets and as a bedding plant, and until recently, cultivars available were only blue- or orange-flowered. Recent breeding research on this species …

Abiotic Retention Of Nitrogen And Dissolved Organic Matter By Forest Mineral Soils, Naoko Watanabe

Master's Theses and Capstones

The effect of NO3- deposition on NH4 +, DON, and DOC retention as well as abiotic NO3 - retention via the hypothesized "ferrous wheel" mechanism was examined in oxic and anoxic tropical and oxic temperate soils using sorption isotherm experiments. Adsorption of NH4+, DON, and DOC by mineral soils was examined in forest floor extracts with DOC levels of 0-50 mg/L. Experimental treatments included no added NO3-, 4mg/L of added NO3-, or 4 mg/L of NO3 - and 2 mg/L of Fe3+. Ferric iron was added since it would likely increase DOC retention in the context of podzolization, and it …

An Experimental Approach To Understanding The Impact Of Vernal Pool Buffer Size On Wood Frogs (Rana Sylvatica), Nicole Alexis Freidenfelds

Master's Theses and Capstones

Strategies to conserve vernal pool-dependent amphibians emphasize the maintenance of adequate areas (buffers) of terrestrial habitat surrounding the pool. I conducted a large-scale field study that specifically examined the effects of buffer width manipulation on adult and metamorph wood frogs at eleven vernal pools in a managed forest in central Maine. Buffer width treatments were 30 m or 100 m forest buffer surrounded by a 100 m clearcut, or no cut (reference). I encircled pools with drift fences and monitored wood frogs from April--November, 2004 and 2005 using pitfall traps and radio-telemetry. Buffer width had no effect on the number …

Effects Of Stz Induced Hyperglycemia And Hyperlipemia On Ppargamma And Abca1 In A Hamster Model Of Atherogenesis, Craig Lamarca

Master's Theses and Capstones

Diabetes mellitus accelerates atherogenesis, but the exact mechanisms involved are unknown. This study examined the effects of hyperglycemia on the inflammatory transcription factor peroxisome proliferator activated receptor gamma (PPARgamma), known to be involved in atherogenesis, and the ATP binding cassette A1 (ABCA1) protein that is a downstream product of PPARgamma. ABCA1 is a membrane bound transporter that is involved in reverse cholesterol transport via high density lipoprotein cholesterol (HDL-C). Hamsters were divided into two groups; hyperlipemic animals fed a high fat/high cholesterol control diet (C) and hyperlipemic/hyperglycemic animals that received the high fat diet and were administered streptozotocin. Half of …

Effects Of Essential Oil Supplementation Of A Corn Silage Based Diet Fed To Lactating Holstein Dairy Cows, Carly Ann Crawford

Master's Theses and Capstones

Thirty multiparous early lactation Holstein cows were used in a randomized complete block design to determine whether the addition of VERTAN (a specific blend of essential oils, major components: thymol, eugenol, vanillin and limonene) to corn silage based diet would increase alter protein metabolism in the rumen and increase milk yield and alter milk composition. Dietary treatments were 0 (control) and 0.08% VERTAN in diet DM. The pretreatment diet (fed 0 to 20 DIM) contained (DM basis) 32.1% corn silage, 14% grass silage, 7.7% alfalfa hay, 0.53% grass hay, 19.4% finely-ground corn, 1.8% beet pulp, 1.8% citrus pulp, 3.6% soybean …

Understanding Changes In Aboveground Biomass And Height With Elevation At Hubbard Brook Experimental Forest, Mariya Schilz

Master's Theses and Capstones

A better understanding of carbon stocks and fluxes is a priority of many national and international agencies, including NASA, NACP, IPCC, and IGBP. (Asrar et al. 2001, IGBP Annual Report, 2003, IPCC Third Assessment Report: Climate Change 2001). Patterns of carbon stocks and fluxes on the land surface are important for studies of the carbon cycle, climate change, and terrestrial ecology. One source of heterogeneity is associated with elevation. In systems around the world, patterns in carbon stocks, fluxes, and the factors that underlie them have well documented relationships with elevation (Korner 1999, Schwarz et al. 2003, Waide et al. …

Effects Of Forest Clear Cutting On Spotted Salamander ( Ambystoma Maculatum) Migration, Jessica Susannah Veysey

Master's Theses and Capstones

Upland buffer zones are a proposed management tool for vernal-pool-breeding amphibians. Substantial validation of buffers, via experimental upland habitat disturbance, is lacking. Specifically, no studies have examined immediate effects of clear cutting on spotted salamander (Ambystoma maculatum ) migration. I used clear cutting to experimentally manipulate upland buffer widths at 11 vernal pools. I then radiotracked 40 adult spotted salamanders at these pools, and modeled their migration with mixed-effects regression. Mean maximum distance from the pool was 106.0 +/- 15.4 m (range = 1.6 to 427.6 m). At clear cut-treatment pools, mean percent of time in the cut was 27.2 …

Environmental Effects On Early Life Stages Of American Shad (Alosa Sapidissima) And Rainbow Smelt (Osmerus Mordax ), Kristen Fuda

Master's Theses and Capstones

Recruitment of American shad (Alosa sapidissima) and rainbow smelt (Osmerus mordax) has steadily declined over the last few decades, possibly due to the construction of physical impediments to migration and increases in anthropogenic pollution. In order to elucidate environmental parameters influencing early life stages of anadromous fish, both laboratory and field studies were conducted. The effects of abiotic factors, including dissolved oxygen (DO), pH, salinity, nitrate, and phosphate, on hatch and survival of larval and juvenile American shad and rainbow smelt were examined in laboratory studies. Field studies on shad emigration were conducted in the Exeter River, and studies on …

An Evaluation Of The Effect Of Terrain Normalization On Classification Accuracy Of Landsat Etm+ Imagery, Jesse B. Bishop

Master's Theses and Capstones

More than 60% of land in New Zealand has been converted from native forests to residential areas, agriculture, or forest plantations. Settlers brought many species of plants and animals to New Zealand. Many native species were unable to protect themselves from these new predators, causing numerous extinctions. In light of this rapid decline in biodiversity, the New Zealand government has attempted to mitigate the destruction of endemic flora and fauna through both new environmental policies and intensive land management. Land management techniques include the restoration of developed land and the protection of remaining areas of native forest. Monitoring of restoration …