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Theses/Dissertations

1970

Loma Linda University Electronic Theses, Dissertations & Projects

Microbiology

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Infectivity And Development Of Trichinella Spiralis In Germ-Free, Monocontaminated And Conventional Mice, Daulat Sitanggang Aug 1970

Infectivity And Development Of Trichinella Spiralis In Germ-Free, Monocontaminated And Conventional Mice, Daulat Sitanggang

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of the experiment was to compare the development of Trichinella spiralis in germ-free, Lactobacillus casei monocontaminated, Escherichia coli monocontaminated and conventional mice. The criteria used in the experiments were the recovery of adults and larvae of T. spiralis after 72 hours and one month post-infection, respectively. which gave an indication of the effects of no bacteria or only a certain, single species of bacterium on the development of this nematode in mice.

Germ-free T. spiralis larvae were obtained by washing them several times with sterile normal saline, and treatment with antibiotics. The gavaging procedure was done without anesthetization …


The Effect Of Immune Igm And Igg On The Infectivity Of Trichinella Spiralis In Mice, Clare K. Kwan Aug 1970

The Effect Of Immune Igm And Igg On The Infectivity Of Trichinella Spiralis In Mice, Clare K. Kwan

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]


Immune Blocking Of Fluorescent Antibody Staining Of Streptococcus Group A Compared With Anti-Streptolysin O, George K. Peck Jun 1970

Immune Blocking Of Fluorescent Antibody Staining Of Streptococcus Group A Compared With Anti-Streptolysin O, George K. Peck

Loma Linda University Electronic Theses, Dissertations & Projects

The fluorescent antibody blocking antibody (FABA) test has been discribed and compared with the conventional antistreptolysin 0 antibody (ASOA) test in the detection of Streptococcus pyogenes group A antibodies in sera samples.

A slide inhibition test was used to detect the presence of FABA in the following manner; (a) fix group A streptococcal cells to a slide, (b) incubate these cells with the patient's serum for thirty minutes, (c) wash off serum with buffered saline and dry, (d) incubate these same cells with group A streptococcal fluorescent tagged antibody for thirty minutes, (e) wash and dry as before, and (f) …


The Effectiveness Of Camphorated Paramonochlorophenol, Metacresyl Acetate, And Prednisolone In Combination As An Antimicrobial Agent, Maurice Cutler Jan 1970

The Effectiveness Of Camphorated Paramonochlorophenol, Metacresyl Acetate, And Prednisolone In Combination As An Antimicrobial Agent, Maurice Cutler

Loma Linda University Electronic Theses, Dissertations & Projects

Post-treatment discomfort following endodontic procedures can be caused by inflammation due to injudicious use of intracanal medicaments. Camphorated paramonochlorophenol, metacresyl acetate, and prednisolone in combination is advocated to reduce the inflammatory potential of the germicide and thus reduce the occurrence of post-operative pain. It is imperative that a medicament retain antimicrobial activity while reducing inflammatory potential.

This investigation was undertaken toward determining on an in vitro quantitative bactericidal versus bacteriostatic basis by use of the broth dilution test tube method whether camphorated paramonochlorophenol, metacresyl acetate, and prednisolone in combination retains antimicrobial activity comparable to selected other commonly used nonspecific germicides. …