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More Than The Sum Of Their Parts: Building A Framework For Understanding Host-Microbe Interactions In Medicago Sativa, Katherine Mackenzie Moccia
More Than The Sum Of Their Parts: Building A Framework For Understanding Host-Microbe Interactions In Medicago Sativa, Katherine Mackenzie Moccia
Doctoral Dissertations
This dissertation seeks to understand plant-microbe interactions in the agriculturally relevant plant Medicago sativa from three distinct vantage points within microbiology. Within the plant microbiome, we examine how primer usage and the application of peptide nucleic acids impacts 16S and 18S rRNA gene sequencing. In doing so, we design a novel peptide nucleic acid, PNA, and test its impact using multiple primers and sequencing protocols. Once microbial sequencing methodology is established, we generate a synthetic consortium of bacterial isolates from M. sativa leaves and modulate nitrogen levels to better understand microbial structure. Drop out communities, where we remove one member …
Extracting Detailed Metabolic Information And Connections From Mammalian Gut Microbiomes Via Metaproteomics, Jose A. Blakeley-Ruiz
Extracting Detailed Metabolic Information And Connections From Mammalian Gut Microbiomes Via Metaproteomics, Jose A. Blakeley-Ruiz
Doctoral Dissertations
A diverse community of bacteria populates the mammalian gastrointestinal tract. These populations exist in a balance with the host assisting with key functions, particularly metabolism of intractable fibers and immune modulation. Disruption of this balance can lead to diseases such as infection, inflammatory bowel syndrome, and obesity. Common symptoms include chronic pain, chronic inflammation, and altered metabolism. Several taxonomic classifications of bacteria have been associated with these diseases, but Recent studies have indicated that these finding are not always statistically valid. An explanation for this is that microbial communities between individuals and even across time can vary substantially even when …
Microbial And Metabolomic Biomarkers Of Feed Efficiency In Angus Cattle, Brooke Ashley Clemmons
Microbial And Metabolomic Biomarkers Of Feed Efficiency In Angus Cattle, Brooke Ashley Clemmons
Doctoral Dissertations
In Tennessee and the United States, cattle account for a significant proportion of the livestock agricultural sector. In the beef cattle industry, 40-70% of the total cost of production comes from feed costs, resulting in billions of dollars going towards feeding cattle. Thus, identifying methods for improving feed efficiency is imperative for reduced feed input costs and more effective use of environmental and economic resources. The rumen microbiome contributes a vast amount of nutrients for the host ruminant, including approximately 70% of energy precursors as well as vitamins, proteins, and other nutrients. These microbes produce metabolites that are then available …
It’S All In The Mix: Physiological Effects Of Fatty Acid Supplementation On Enterococcus Faecalis, William Thomas Brewer V
It’S All In The Mix: Physiological Effects Of Fatty Acid Supplementation On Enterococcus Faecalis, William Thomas Brewer V
Masters Theses
Enterococcus faecalis is a Gram-positive bacterium that normally exists as an intestinal commensal in humans. Additionally, it can survive in the environment for extended periods and cause infections in immunocompromised hosts, making E. faecalis a leading cause of nosocomial infections. Previous work noted that specific fatty acids found in serum contribute to tolerance of membrane damaging agents, including the drug daptomycin. However, not all fatty acids found in serum were able to induce such protection. Herein, we measured a wide array of physiological responses after supplementation with combinations of protective (i.e., induced daptomycin tolerance) and non-protective fatty acids. When supplementing …