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Development Of Cryopreservation Methods For Sperm Of Pinto Abalone (Haliotis Kamtschatkana) For Conservation Aquaculture., Caitlin O'Brien
Development Of Cryopreservation Methods For Sperm Of Pinto Abalone (Haliotis Kamtschatkana) For Conservation Aquaculture., Caitlin O'Brien
WWU Graduate School Collection
The pinto abalone, Haliotis kamtschatkana, is an ecosystem engineer with cultural and ecological significance. Due to the dramatic 98% decline in populations, a ten-year recovery plan was enacted to bring levels of pinto abalone back from the brink of extirpation in the San Juan Archipelago, Washington. The focus of this study was to assist restoration efforts in creating self-sustaining populations by developing methods of cryopreservation for male pinto abalone sperm. My study evaluated three commonly used cryoprotectants, a series of freeze/thaw temperatures, and developed methods of quality assessment specific to pinto abalone. Animals were provided and cared for by …
Optimizing Abalone Outplant Strategies Using Larval Haliotis Kamtschatkana In The San Juan Archipelago, Washington, Katherine Mills-Orcutt
Optimizing Abalone Outplant Strategies Using Larval Haliotis Kamtschatkana In The San Juan Archipelago, Washington, Katherine Mills-Orcutt
WWU Graduate School Collection
Prior studies testing the use of Haliotis kamtschatkana larvae as a means of stock enhancement in the San Juan archipelago have shown no success in inducing larvae to settle in densities required for successful reproduction. I conducted an experiment to test the effectiveness of new methods for outplanting larval abalone in hopes of creating a protocol Puget Sound Restoration Fund could use to restore wild populations. Using two sites within the San Juan archipelago, I outplanted abalone into pre-constructed larval abalone modules (LAMs) and sampled over four months, testing retention strategies. Three types of LAMs were used at each site …