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Understanding Crx-Associated Retinopathies Using Animal Models, Nicholas Minh Abell Tran

All Theses and Dissertations (ETDs)

Cone-rod homeobox: CRX) protein is a "paired-like" homeodomain transcription factor that is essential for regulating rod and cone photoreceptor transcription. Mutations in human CRX are associated with the dominant retinopathies Retinitis Pigmentosa: RP), Cone-Rod Dystrophy: CoRD) and Leber Congenital Amaurosis: LCA), with variable severity. The goal of my dissertation project was to develop and characterize animal models to understand genetic mechanisms of phenotypic diversity in CRX-associated disease. Heterozygous Crx Knock-Out: KO) mice: "+/-") have normal vision as adults and fail to model the dominant human disease.

We generated two Crx Knock-IN: K-IN) mouse models: CrxE168d2: "E168d2") and CrxR90W: "R90W"), which …

Population Demographics And Genetic Structure Of Black Bears In Coastal Louisiana, Jesse Charles Troxler

Masters Theses

The range and abundance of the Louisiana black bear (Ursus americanus luteolus) were greatly diminished during the 20th century. This subspecies was reduced to 3 small, isolated subpopulations in Louisiana as bottomland hardwood habitat was converted to agriculture. These bears were listed as threatened by the U.S. Fish and Wildlife Service in 1992 and a recovery plan was published in 1995. Recovery requires estimates of population parameters to evaluate current population status and future viability. I conducted a mark-recapture study from 2010 to 2012 to estimate demographic parameters of the coastal population of Louisiana black bears. Because inbreeding …

Insights Into The Function Of The Fatc Domain Of Saccharomyces Cervisiae Tra1 Via Mutation And Suppressor Analysis, Samantha A. Pillon

Electronic Thesis and Dissertation Repository

The regulation of transcription is an important cellular function because it is the first step in gene regulation. In Saccharomyces cerevisiae, two protein complexes, SAGA and NuA4, act as regulators of transcription. A common protein shared between these two complexes, called Tra1, regulates transcriptional activation through its interaction with gene specific transcriptional activators. Tra1 is a member of the PIKK family of proteins, which are characterized by FAT, PI3K and FATC domains. The FATC domain encompasses the terminal 33-35 residues of the protein. Two mutations within the FATC domain, tra1-L3733A and tra1-F3744A, result in slow growth under stress …

The Ecology And Genetics Of Schoenoplectus Maritimus, An Important Emergent Macrophyte, Across Diverse Hydrologic Conditions—Implications For Restoration, Amanda Clare Sweetman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Wetlands in the Intermountain West are typically dominated by large monotypic stands of emergent wetland plants, are highly productive, and support millions of migratory birds as important stops along the Pacific Flyway. In systems with low species diversity, such as these, diversity within a species (intraspecific diversity) can play an important role in population fitness and ecosystem functioning and can impact restoration success. Our research was designed to inform future restoration and management activities by studying the pattern of diversity within and among natural plant populations, and by studying how hydrology and plant materials used in restoration (source and diversity …

Apoptotic And Epigenetic Induction Of Embryo Failure Following Somatic Cell Nuclear Transfer, Aaron Patrick Davis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The cloning of domestic species has tremendous potential, as the technology can be used in selective breeding, conservation, and the production of transgenic animals. The technique of cloning involves the transplant of DNA from a cell to a recipient gamete. Following transfer to a surrogate, the cloned embryo may successfully complete development to a live offspring. Despite intensive research, the success rate of cloning remains prohibitively low, and the potential benefits of cloning have not yet been realized. Embryos produced from cloning suffer from high rates of embryo degradation, implantation failure, and abortion. The goal of this dissertation research project …

Parentage Analysis And Conservation Genetics Educational Material For The Eastern Hellbender Salamander, Cryptobranchus Alleganiensis Alleganiensis, Sarah A. Chudyk

Master's Projects

Populations of the Eastern hellbender salamander, Cryptobranchus alleganiensis alleganiensis, are declining, making this a species of special concern in New York State and under consideration for Federal Endangered Species listing. As a result of this decline, the New York State Department of Environmental Conservation and the Buffalo Zoo initiated a headstarting program with an egg mass found in the Allegheny River drainage. The juveniles being raised by the Zoo will be released back into the watershed and so understanding the genetic diversity and parentage of these hellbenders will inform the reintroduction efforts. Furthermore, in order to determine how to …

Molecular Technologies In The Science And Policy Of Florida Largemouth Bass Micropterus Floridanus Management In Florida, Josh Sakmar

USF Tampa Graduate Theses and Dissertations

Advances in molecular technologies have provided conservation biologist with the opportunity to quantify the genetic structure of a population and, in turn develop management guidelines and policies aimed at preserving the genetic diversity of fish stocks challenged by human activities. This thesis examines the status of genetics as applied to the management of freshwater fisheries by state natural resource agencies with a purpose of understanding the keys to a successful genetics program. An online survey was used to investigate the breadth of molecular marker application to freshwater fisheries management by state natural resource departments. Seven questions were posed to 50 …