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De Novo Transcriptome Analysis Of The Marine Sponge Cinachyrella Spp: A Potential Model Organism For Oil And Dispersant Ecotoxicology, Emily Smith
HCNSO Student Theses and Dissertations
In order to study the potential effects of an oil spill on coral reef organisms, the marine sponge, Cinachyrella spp. was investigated. In this study, Cinachyrella spp. was placed in a closed aquaculture system and exposed to sub-lethal water-accommodated fractions (WAFs) of Macondo crude oil and chemically-enhanced water accommodated fractions (CE-WAFs) of the dispersant, Corexit 9500, over a 24-hour time course, in order to model the BP Deepwater Horizon oil spill and oil spill sponge response. Illumina RNA sequencing and gene expression analysis utilizing hierarchical clustering, principal component analysis, and KEGG bioinformatic database generated 34,147 unique transcripts with …
Study Of Secondary Metabolite Gene Expression In Marine Microbial Co-Cultures Using Quantitative Real-Time Pcr, Crystal A. Conway
Study Of Secondary Metabolite Gene Expression In Marine Microbial Co-Cultures Using Quantitative Real-Time Pcr, Crystal A. Conway
HCNSO Student Theses and Dissertations
Interactions among microbial organisms often cannot be observed directly, but they can be inferred genetically using new molecular techniques. The analysis of secondary metabolite gene expression produced by co-cultured marine microbial species allows us to see how these organisms interact with one another when kept in the same environment. Co-cultures of three different strains of marine bacteria, P. aeruginosa PAO1, Roseobacter denitrificans OCH114, and Salinispora arenicola CNS-205 were grown in a laboratory setting, and using the Real-Time qPCR method gene expression levels of two different secondary metabolite producing genes from each organism was accessed across three time points. P. aeruginosa …