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Effect Of Guanosine Tetraphosphate On Growth Rate And Stable Rna Synthesis In Escherichia Coli, Heather Anne Quintanilla
Effect Of Guanosine Tetraphosphate On Growth Rate And Stable Rna Synthesis In Escherichia Coli, Heather Anne Quintanilla
Theses and Dissertations - UTB/UTPA
Guanosine tetraphosphate (ppGpp) is a good candidate for a stable RNA synthesis regulator at different growth rates because: high ppGpp levels present during the stringent response are known to inhibit stable RNA synthesis, and there is an inverse correlation between ppGpp concentration and stable RNA synthesis at different growth rates. A system was constructed allowing the user to control intracellular ppGpp levels within the range characteristic of exponentially growing strains, while not allowing cells to adjust ppGpp in response to environmental changes. E. coli strains were transformed with a plasmid containing a truncated relA allele to provide ppGpp. Results show …
Ocelot Distribution In The Lower Rio Grande Valley National Wildlife Refuge, Kevin James Shinn
Ocelot Distribution In The Lower Rio Grande Valley National Wildlife Refuge, Kevin James Shinn
Theses and Dissertations - UTB/UTPA
Weaver hair-snaring surveys were conducted at 125 sites on 27 Refuge tracts to obtain information about the distribution and population status of the endangered ocelot ( Leopardus pardalis ) in the Lower Rio Grande Valley National Wildlife Refuge. The surveys resulted in 33.6% of the pads “hit”, including 8 by ocelot and 29 by bobcat. There was no significant difference in the number of ocelot hits between warm and cool seasons or between the sexes. Preferred habitat consists of dense thornscrub brush. Four species, snake-eyes, granjeno, honey mesquite, and colima comprise 50% or more of the total cover at each …
Identification Of A Genetic Locus Involved In Ppgpp Metabolism In Escherichia Coli, Roxanne Enid Garza
Identification Of A Genetic Locus Involved In Ppgpp Metabolism In Escherichia Coli, Roxanne Enid Garza
Theses and Dissertations - UTB/UTPA
Two genes involved in the metabolism of ppGpp are relA and spoT . A relX mutant has altered ppGpp levels, so relX may be a regulator of the RelA or SpoT enzymes. The growth of the relX mutant has been reported to be both temperature and leucine sensitive. It was discovered in this work that this relX mutant is not temperature sensitive, however it is leucine sensitive. The original goal was to study effects of the relX gene on ppGpp metabolism. During the process of mapping the relX gene, it was discovered that the leucine sensitivity was caused by some …