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Physiological And Biochemical Aspects Of Agrobacterium-Wheat (Triticum Aestivum L.) Interactions, David L. Parrott Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Agrobacterium tumefaciens and A. rhizogenes are the causal agents of gall or hairy root disease, but normally the bacteria do not cause disease in wheat. However, both bacteria grew without inhibition when exposed to intact or wounded wheat roots or embryos, and they colonized wheat root surfaces to levels similar to dicotyledonous plants. A. tumefaciens and A. rhizogenes induced 23% cell death after a 1-h exposure to wheat embryo cells grown in 7.4 mM O₂, while the extent of cell death at 2.1 mM O₂ was 8%. Contact with A. tumefaciens or A. rhizogenes caused cultured wheat …