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Articles 1 - 6 of 6
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Comparative Study Of Hpv 16 And Hpv 18 Antibody Detection In Serum, Cervical Mucus, And Oral Mucosal Transudate, Emily Lauren Blalock
Comparative Study Of Hpv 16 And Hpv 18 Antibody Detection In Serum, Cervical Mucus, And Oral Mucosal Transudate, Emily Lauren Blalock
Biology Theses
Measuring HPV exposure relies on detection of HPV type-specific antibodies, but methods are not standardized. Additionally, there is little information on the best sample type for HPV antibody detection. This study validated pseudovirion neutralization (PVN) assay for HPV antibody detection and compared it to IgG ELISA. Both assays were applied to paired serum and cervical mucus samples. Additionally, PVN assay was utilized to evaluate the feasibility of oral mucosal transudate (OMT) samples to monitor the HPV immune response. Serum was more likely to be positive on PVN assay than on IgG ELISA (p= 0.025). Both assays correlated with HPV-16 DNA …
The Production Of Designed Potential Protein Contrast Agents And Their Encapsulation In Albumin Microspheres, Julian A. Johnson
The Production Of Designed Potential Protein Contrast Agents And Their Encapsulation In Albumin Microspheres, Julian A. Johnson
Biology Theses
Using protein design, a series of metal binding proteins have been designed, allowing the local factors that contribute to metal affinity and thermostability to be studied. Those proteins with the highest metal binding affinities had the lowest apo-form Tm and the largest ÄTm upon metal binding. In this thesis, major steps have been taken toward applying the engineered protein to MR imaging. The progress of magnetic resonance imaging is hindered by low specificity and rapid elimination of FDA-approved MRI contrast agents. The engineered protein contrast agent has been conjugated to a cancer-specific targeting peptide and encapsulated in albumin microspheres to …
Production Of B Virus Glycoprotein D And Evaluation Of Its Diagnostic Potential, Chadi N. Filfili
Production Of B Virus Glycoprotein D And Evaluation Of Its Diagnostic Potential, Chadi N. Filfili
Biology Theses
B virus diagnosis presents a challenge largely complicated by the asymptomatic infection of rhesus macaques, and extremely pathogenic fatal infections in humans. Humoral detection of antibodies is generally performed using whole virus antigen for which preparation requires strict biosafety measures and specialized BSL-4 facilities. As an alternative to utilizing B virus antigen, we describe the production of a truncated form of B virus envelope glycoprotein D, gD 287, in a baculovirus expression system, and evaluate its diagnostic potential as an antigen in recombinant ELISA. After purification and characterization, gD 287 was tested using 22 negative and 72 positive macaque sera …
The C-Phycocyanin/Beta Protein Inhibits Cancer Cell Proliferation, Haizhen Wang
The C-Phycocyanin/Beta Protein Inhibits Cancer Cell Proliferation, Haizhen Wang
Biology Theses
C-Phycocyanin (C-PC) from blue-green algae has been reported to have various pharmacological characteristics, including anti-inflammatory and anti-cancer activities. In this study, the beta-subunit of C-PC (ref to as C-PC/beta) was expressed and purified from bacteria E. coli BL-21. The recombinant C-PC/beta has been demonstrated to have anticancer properties. Under the treatment of 5 microM of the recombinant C-PC/beta, four different cancer cell lines accrued a high proliferation inhibition and apoptotic induction. The C-PC/beta interacts with membrane-associated beta-tubulin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been found. Under the treatment of the C-PC/beta, depolymerization of microtubulin and actin-filament was observed. The cells underwent …
Mapping And Filling Metabolic Pathway Holes, Dipendra Kaur
Mapping And Filling Metabolic Pathway Holes, Dipendra Kaur
Biology Theses
The network-mapping tool integrated with protein database search can be used for filling pathway holes. A metabolic pathway under consideration (pattern) is mapped into a known metabolic pathway (text), to find pathway holes. Enzymes that do not show up in the pattern may be a hole in the pattern pathway or an indication of alternative pattern pathway. We present a data-mining framework for filling holes in the pattern metabolic pathway based on protein function, prosite scan and protein sequence homology. Using this framework we suggest several fillings found with the same EC notation, with group neighbors (enzymes with same EC …
Reinforcing Efficacy Of Amphetamine In Adolescent And Adult Male Rats, Lauren Chantel Payne
Reinforcing Efficacy Of Amphetamine In Adolescent And Adult Male Rats, Lauren Chantel Payne
Biology Theses
Rationale: Amphetamine abuse by adolescents predicts long-term drug dependence. Heightened vulnerability to drug abuse could be due to higher sensitivity to drug’s reinforcing effects. Rodents are used to study age-related sensitivities to drugs. Objective: We compared intravenous amphetamine self-administration between adolescent and adult male rats on an operant schedule of reinforcement measuring the reinforcing efficacy of a drug. Methods: After surgery, adolescent and adult rats acquired lever-pressing behavior reinforced by amphetamine infusions. Results: Both age groups exhibited more infusions per session as dose increased. However, neither the number of infusions per session nor total amphetamine intake differed across age groups. …