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The Measurement Of Angiotensin-Converting Enzyme In Human Serum, Andrew P. Kenny
The Measurement Of Angiotensin-Converting Enzyme In Human Serum, Andrew P. Kenny
Theses
The measurement of angiotensin converting enzyme using the substrate FAPGG was investigated with a view to optimising its performance. It was found that a 100 mnol/l triethanolamine-HCl buffer gave a significantly higher absorbance change then the existing TRIS-HCl in the method. As this buffer has not been previously described in the measurement of ACE it was decided to introduce this buffer into a kinetic procedure and evaluate the modified method. The reference interval for the triethanolamine Method was determined by measuring the ACE activity on 200 serum from blood donors and a significant difference (P < 0.05) in the SACE activity of male and female donors was observed.
The modified method was found …