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The Influence Of Fluorescent Light On The Development Of In Vitro Fertilized Bovine Oocytes, Jared Bunch Aug 1996

The Influence Of Fluorescent Light On The Development Of In Vitro Fertilized Bovine Oocytes, Jared Bunch

Undergraduate Honors Capstone Projects

Advances in in vitro fertilization and in vitro culture techniques have allowed considerable progress in identifying physiological requirements of mammalian embryos. Parrish et al. (11) reported a major breakthrough on in vitro fertilization when his group identified heparin as an important factor for the capacitation of spermatozoa. Capacitation is necessary for fertilization of matured oocytes. During the precoculture era of embryos (prior to 1980), the development of early preirnplantation embryos was vary limited regardless of medium, medium supplement, gas atmosphere, osmolarity or pH used (7). Coculture techniques of fertilized oocytes using somatic cells during in vitro production, particularly of bovine …


No Evidence Of Linkage Between 35 Genetic Markers And The Spider Lamb Syndrome Gene In Sheep, Sarah Thornell South May 1996

No Evidence Of Linkage Between 35 Genetic Markers And The Spider Lamb Syndrome Gene In Sheep, Sarah Thornell South

Undergraduate Honors Capstone Projects

Spider Lamb Syndrome (SLS) is a semi-lethal congenital disorder affecting the Suffolk and Hampshire sheep breeds. The clinical manifestations of the syndrome include severe skeletal and muscular abnormalities with long, fine-boned legs, curvature of the spine and nasal septum, minimal muscling and severe muscle atrophy. The affected animals have a difficult time walking and most lambs do not survive past the first six months of life. A single autosomal recessive gene causes the disorder. Researchers believe the SLS gene arose as a single source mutation in a popular Suffolk blood line in the 1970s. Heterozygous carriers of the SLS gene …


Root Colonization By Pseudomonas Putida: The Role Of Catalase And Superoxide Dismutase Isozymes, Tawnya Lynn Bowles May 1996

Root Colonization By Pseudomonas Putida: The Role Of Catalase And Superoxide Dismutase Isozymes, Tawnya Lynn Bowles

Undergraduate Honors Capstone Projects

The fluorescent soil bacterium Pseudomonas putida is an aggressive root colonizer (1) that offers benefits to the host plant by suppressing soilborne pathogens and enhancing plant growth (12,13). Upon introduction in the field through seed inoculation, certain isolates of P. putida have the potential to act as an effective biological control agent (3). P. putida has also been shown to promote the growth of potatoes, sugarbeets, and radishes (13). Fluorescent pseudomonads are effective in protecting plants against pathogens such as Gaeumannomyces graminis var. tritici, a fungus that causes the root disease of wheat called take-all (13).


Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird May 1996

Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird

Undergraduate Honors Capstone Projects

The cytomegalovirus (CMV) is capable of causing serious illness and death in immunocompromised individuals. The objective of this research is to develop a method of detecting antibodies against CMV. This has lead to the development of an Enzyme linked Immunosorbent Assay (ELISA) that is capable of detecting CMV or anti-CMV antibodies. The process in the development of such an assay involves; 1) the purification of Hyperimmune Rabbit Serum containing anti-CMV antibodies, 2) determination of the appropriate concentrations necessary to provide a clear and concise response to the test, and 3) testing of monoclonal antibodies to determine their specificity towards the …


The Use Of The Xylose Tolerance Test As An Indicator Of Malabsorption In Cryptosporidiosis, James A. Evans May 1996

The Use Of The Xylose Tolerance Test As An Indicator Of Malabsorption In Cryptosporidiosis, James A. Evans

Undergraduate Honors Capstone Projects

Cryptosporidium parvum, a protozoan parasite infecting epithelial cells lining the intestinal tract of animals and humans, causes fulminate diarrhea and malabsorption of essential nutrients following damage to the cellular brush border. The present study was undertaken to evaluate the use of a pentose sugar (D-xylose) in a standardized tolerance test as an indicator of malabsorption due to cryptosporidiosis in the dexamethasone (DEX)-immunosuppressed adult C57BL/6N mouse model. One group containing 14 experimental mice (in addition to appropriate control groups) was immunosuppressed using daily intraperitoneal injections of DEX (125 μg/mouse). On day 7 postimmunosuppression, mice were inoculated orally with 104 C. …


Environmental Mediation: An Assessment And Case Study Of The Logan Canyon Highway Controversy, Jared Glenn Parkinson May 1996

Environmental Mediation: An Assessment And Case Study Of The Logan Canyon Highway Controversy, Jared Glenn Parkinson

Undergraduate Honors Capstone Projects

For a little more than twenty years, alternative methods of dispute management have been employed to address various environmental conflicts in order to avoid the more traditional method of litigation. Mediation is a voluntary process which involves face-to-face negotiation among parties and the use of a neutral third-party negotiator. Environmental mediation has helped manage disputes related to land use, natural resource management, use of public lands, water resources, energy development, air quality and toxins (Bingham 1986).


Effects Of Copper On Oxidative Stress And Growth Of Pseudomonas Putida, Tiffany Marshall May 1996

Effects Of Copper On Oxidative Stress And Growth Of Pseudomonas Putida, Tiffany Marshall

Undergraduate Honors Capstone Projects

Oxygen is an essential compound in all aerobic systems; however, it can also take part in toxic reactions involving active oxygen species (AOS) and transition metals. Most of the time, antioxidant enzymes act as defense mechanisms and keep the production of AOS to a minimum. However, if the defense mechanisms are not adequate, damage may occur and possibly lead to the breakdown of biomolecules such as DNA, lipids, and proteins that are vital to both simple and complex organisms. Oxidative damage could also be a contributing factor to many human pathological conditions including, aging, atherosclerosis, carcinogenesis, diabetes mellitus, lung cancer, …