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Use Of Conductance Technology To Determine The Bioactivity Of Oxytetracycline-Hydrochloride, Michael Mc Donagh Jan 1996

Use Of Conductance Technology To Determine The Bioactivity Of Oxytetracycline-Hydrochloride, Michael Mc Donagh

Theses

The biological activity of oxytetracycline-hydrochloride was investigated using the indirect conductivity method which is based on the measurement of metabolically generated CO2. A number of laboratory strains and a fresh natural inoculum were exposed to oxytetracycline in the presence and absence of 12% sterile sediment. Results comparing sediment and sediment free systems for all test cultures revealed elevated minimum inhibitory concenrations (MIC) of the agent in the presence of sediment; percentage bioactivity results ranged between 0.50 and 2.07%. pH differences betw'een sediment and sediment-free systems were found not to effect MIC results, and the use of different buffers resulted in …


Acidic Marine Biotoxins Implicated In Diarrhetic Shellfish Poisoning, Eoin P. Carmody Jan 1996

Acidic Marine Biotoxins Implicated In Diarrhetic Shellfish Poisoning, Eoin P. Carmody

Theses

An okadaic acid (OA) etiology has been demonstrated for most outbreaks of diarrhetic shellfish poisoning (DSP) in Europe, An isomer of OA, dinophysistoxin-2 (DTX-2), has recently been found in Irish waters and, in 1991 and 1994, was the major toxin during prolonged infestations in cultivated mussels (Mytilus edulis) along the south-west coast of Ireland.

The OA class of DSP toxins have been analysed by HPLC using modified versions of three established fluorescent derivatisation methods. A ‘dual tag’ method for the confirmation of specific toxins has also been developed.

Survey work has been carried out over the past four years on …


Development Of A New Hydroseeding Process For Soil Stabilisation And Vegetation, Eileen T. Keane Jan 1996

Development Of A New Hydroseeding Process For Soil Stabilisation And Vegetation, Eileen T. Keane

Theses

A variety of mechanical and chemical extraction procedures were used to produce crude alginate gels from 3 seaweed species. These viscous hydrocolloidal substances had excellent adhesive properties but did however prove phytotoxic to germinating seeds. Experimental gels were subsequently refined and germination trials were carried out to determine optimum gel types. Pilot field trials were set up to compare the 2 most favourable gels with existing commercial hydroseeding products imported from Norway and Sweden. These tests involved the use of experimental plots on loam and sand substrates with slopes of 0^, 22^ and 45^. A number of biodegradable organic substances …