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Spermiogenesis In Two Male-Sterile Third-Chromosome Mutants Of Drosophila Melanogaster, Richard F. Wilkinson
Spermiogenesis In Two Male-Sterile Third-Chromosome Mutants Of Drosophila Melanogaster, Richard F. Wilkinson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
An ultrastructural study of sperm differentiation was made on two male-sterile mutants of Drosophila melanogaster. The mutations were induced on the third chromosome with the monoalkylating agent ethyl methanesulfonate and were maintained in stock cultures over a balancer chromosome using a system of multiple inversions. The stages of sperm differentiation, a most dramatic form of cellular differentiation, were then studied at the electron microscope level to determine the effect of the mutations on sperm structure and to correlate those changes with normal sperm development.
Studies of the genetic control of cellular differentiation have been hampered by difficulties in devising …
Induction And Initial Characterization Of Male-Sterile Mutants On The Second Chromosome Of Drosophila Melanogaster, Susan M. Graves
Induction And Initial Characterization Of Male-Sterile Mutants On The Second Chromosome Of Drosophila Melanogaster, Susan M. Graves
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Fourteen recessive, male-sterile, autosomal mutants of Drosophila melanogaster were induced using ethyl methanesulfonate. The techniques employed restricted the recovery of mutants to those located on the second chromosome. Eleven of the mutants were male-sterile at 23°C, and the remaining three, though fertile at 23°C, were male-sterile at 28°C. Complementation tests involving all possible pairs of mutations showed each to be located in a unique cistron.
Spermiogenesis in each mutant was studied by light microscopy to determine the stage at which cellular differentiation deviates from normal and to characterize the associated gross abnormalities. Phase contrast microscopy showed that each mutant sterile …