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Connecting Environment And Phenotype: Deciphering Mechanisms Of Diapause Entry And Exit In The Annual Killifish Austrofundulus Limnaeus, Erin Monica Davis
Connecting Environment And Phenotype: Deciphering Mechanisms Of Diapause Entry And Exit In The Annual Killifish Austrofundulus Limnaeus, Erin Monica Davis
Dissertations and Theses
Embryonic development is complex, dynamic, and dependent on environmental factors. Mechanisms of sensing and integrating environmental stimuli are diverse, and understanding these mechanisms in extant species can elucidate how complex phenotypes emerge from genomic information expressed in an environmental context. In Austrofundulus limnaeus, an annual killifish with alternative developmental trajectories, light and temperature are vital factors that determine if an embryo will enter a state of diapause. We hypothesize that embryos of A. limnaeus use the vitamin D3 signaling pathway as a vehicle to incorporate these ecological signals into their developmental programing. Here we provide evidence of …
Transcriptomic Regulation Of Alternative Phenotypic Trajectories In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Amie L. Romney
Transcriptomic Regulation Of Alternative Phenotypic Trajectories In Embryos Of The Annual Killifish Austrofundulus Limnaeus, Amie L. Romney
Dissertations and Theses
The Annual Killifish, Austrofundulus limnaeus, survives the seasonal drying of their pond habitat in the form of embryos entering diapause midway through development. The diapause trajectory is one of two developmental phenotypes. Alternatively, individuals can "escape" entry into diapause and develop continuously until hatching. The alternative phenotypes of A. limnaeus are a form of developmental plasticity that provides this species with a physiological adaption for surviving stressful environments. The developmental trajectory of an embryo is not distinguishable morphologically upon fertilization and phenotype is believed to be influenced by maternal provisioning within the egg based on observations of offspring phenotype …
Developmental Mechanisms That Support Genome Stability And Embryonic Survival In Stress-Tolerant Embryos Of The Annual Killifish Austrofundulus Limnaeus, Josiah Tad Wagner
Developmental Mechanisms That Support Genome Stability And Embryonic Survival In Stress-Tolerant Embryos Of The Annual Killifish Austrofundulus Limnaeus, Josiah Tad Wagner
Dissertations and Theses
In order to complete their life cycles, vertebrates require oxygen and water. However, environments are not always forgiving when it comes to constantly providing these basic needs for vertebrate life. The annual killifish Austrofundulus limnaeus is possibly the most well described extremophile vertebrate and its embryos have been shown to tolerate extremes in oxygen, salinity, and water availability. This phenotype is likely a result of the annual killifish life history, which includes periods of temporary habitat desiccation and oxygen deprivation, and requires the production of stress-tolerant embryos that depress metabolism in a state of suspended animation, known as diapause. Over …
The Effects Of Hypoxia And Temperature On Developing Embryos Of The Annual Killifish Austrofundulus Limnaeus, Skye N. Anderson
The Effects Of Hypoxia And Temperature On Developing Embryos Of The Annual Killifish Austrofundulus Limnaeus, Skye N. Anderson
Dissertations and Theses
Little is known about the physiology or biochemistry of hypoxia (reduced levels of oxygen) tolerance during development in vertebrate embryos. In most species, relatively brief bouts of severe hypoxia are lethal or teratogenic. An exception to such hypoxia intolerance is the annual killifish Austrofundulus limnaeus, in which populations persist in hypoxic environments. This species inhabits seasonal ponds in Venezuela, surviving through the dry season in the form of diapausing embryos. Embedded in the pond sediment, embryos of A. limnaeus are routinely exposed to hypoxia and anoxia (lack of oxygen) as part of their normal development. Here, we exposed embryos to …