Digital Commons Network^™

The Isolation And Characterization Of A Novel Iron Protein From Desulfovibrio Gigas, Alan Kay Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The isolation, purification, and partial characterization of a novel iron-containing protein from the sulfate-reducing anaerobic bacterium, Desulfovibrio gigas, is described. The highly insoluble protein was isolated from the cell debris following osmotic shock of the bacteria. The insoluble fraction consistently contained about 90% of the cell-associated iron. This protein was treated with acid, chelating agents, detergents and proteases in order to study their effect on the iron solubility. The results of elemental analysis of a crude protein preparation were 5.3% iron, 2.9% sulfur and 11 .9% nitrogen. An independent colorimetric iron analysis showed 6.4% iron. The iron could be …

The Characterization Of Carbamate Resistance In The Colorado Potato Beetle, Leptinotarsa Decemlineata (Say), In Comparison With The House Fly, Musca Domestica (L), Randy L. Rose

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Resistant and susceptible populations of the Colorado potato beetle, Leptinotarsa decemlineata (Say), and the house fly, Musca domestica (L), were compared with respect to carbofuran and carbaryl toxicity in the presence and absence of the synergist, piperonyl butoxide. Resistance levels of the New Jersey population when compared with the susceptible Logan population by topical application of carbaryl and carbofuran were > 833 and 820, respectively. A resistance level of 583 was determined from carbofuran bioassays of Rutgers and NAIDM house flies. Similar levels of resistance development between these species suggests the possibility that similar resistance mechanisms may be involved.

Utilization of …

Population Characteristics And Movement Patterns Of Cougars In Southern Utah, Thomas P. Hemker

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Movements of 22 cougars (Felis concolor) were monitored by radio-telemetry between January 1979 and July 1981 in southern Utah. The population, composed of resident, transient, and juvenile cougars, remained relatively constant in size for 3 years. Densities (0.4-0.5 cougars/100 km²) were considerably lower than has been reported elsewhere. Average annual home area size of resident females (685 km²) and a single resident male (826 km²) were substantially larger than other home area sizes reported. Home areas of resident females overlapped and resident male home areas may have overlapped as well. Despite the …

Effect Of Three Methods Of Processing Barley On Intake And Production Of Lactating Cows, Carlos Garcia Jauregui

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Twenty-four lactating cows were randomly assigned to three treatments within eight each 3 x 3 latin squares with three periods of 21 days duration. Three processing treatments of barley were 1) steam-rolled, 2) ground (fine), and 3) soak-rolled (soaked in water for appr. 24 hours, rolled, and fed within 48 hours). All rations were fed ad libitum and were comprised of 24% alfalfa hay, 16% corn silage, 35.5% barley, 12% whole cottonseed, 12% wheat bran, 0.3% salt, and 0.3% dicalcium phosphate on a dry matter (DM) basis. Electronic doors were used to collect individual feed intake data. Rations and feces …

Nutrient Requirements Of Ruminants In Developing Countries, Leonard C. Kearl

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This book was assembled using the latest information for compiling the nutrient requirements of sheep, goats, cattle and water buffalo. Because of the complexity of the interactions between an animal, its diet, its physiological state, and the environment; the values depicting the nutrient requirements are estimates and not absolutes. These values, however, are considered to be adequate to meet the minimum requirements of healthy animals maintained in a thermoneutral environment.

The mean energy and protein values used in the nutrient tables were arrived at from combining values found in the literature and those provided in personal communication with animal nutritionists …

Estimating Esterase And Monooxygenase Detoxication By Bioassay, Electrophoresis And Metabolic Studies In Pea Aphid, Acrythosiphon Pisum (Harris) Populations (Homoptera: Aphididae), Deifalla H. Al-Rajhi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The role of esterases and monooxygenase in the detoxication vii processes of field populations of pea aphids was studied in relation to seasonal changes, developmental stage, and treatment with DEF and piperonyl butoxide. LCSO values for 12 hour exposure periods in glass vials ra nged from 0.04-2.3 micrograms / vial and 820-4871 J.Jg/vial for paraoxon and carbaryl, respectively. Topical bioassays showed the adults to be more tolerant to paraoxon (LDSO of 1. 4-12.3 ng/aphid) toxicity than nymphs (LDSO of 0.13-4. 7 ng/aphid).

Treatment with DEF® (S,S.S-tributylphosphorotrithioate) enhanced paraoxon toxicity against the pea aphid whereas piperonyl butoxide had little …

Evaluation Of Allergic Hypersensitivity To 2,4-D, Malathion, And Captafol In Mice, Janette R. Cushman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

2,4-D-specific IgE antibodies were detected in serum of BALB/c mice using the rat passive cutaneous anaphylaxis (PCA) test following the second intraperitoneal immunization with 1, 10, or 100 ug of 2,4-D0keyhole limpet hemocyanin (KLH) conjugate administered with aluminum hydroxide adjuvant. The groups that received 1 ug of 2,4-D conjugate had the highest antibody titers (geometric mean of 60). A paper radioallergosorbent test (PRAST) was developed for determination of 2,4-D-specific IgE. The PRAST was equally as sensitive as and showed a positive correlation with the PCA assay.

The anhydride of the diacid metabolite of malathion (MMA) coupled to KLH elicited MMA-specific …

A Study Of Cell Dimensions, Amyloplast Position And Certain Physiological Responses During Gravitropic Bending Of Dicot Stems, Julianne E. Sliwinski

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

If a plant is positioned horizontally, the elongating region responds by bending upward within 10 to 12 h until it is vertical, forming a 90 bend with the stem below. If a Xanthium strumarium L. (cocklebur) plant is placed horizontally, but restricted to that position for 48 h and then released, the bend to the vertical usually takes place within 10 s, suggesting that bending energy is stored in restricted stems. Some plants that do not bend completely to 90° within 10 s do so within 5 min, and other plants can overshoot the 90 mark by as much …

Influence Of Phosphorus And Water Management On Mycorrhiza And Rhizobia In Symbiosis With Legumes, David C. Ianson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Mycorrhizal associations are very important to plant growth. This is mainly due to the hyphal stands that are able to utilize concentrations of nutrients that would normally be considered unavailable for plant growth, especially phosphorus. These associations are of great importance in soils that have been disturbed by mineral extraction and are consequently low in available nutrients. The ability of mycorrhizal fungi to form associations on disturbed sites may be dictated by a number of factors: moisture, soil pH, soil fertility (including heavy metal ions), salinity, and plant response.

Two factorial experiments were conducted with the legume Hedysarum boreale cv …

Seed Case Studies On Atriplex Gardneri (Moq) Dietr.: Bracteole Cell Wall Composition And Enzymatic Degradation, Priscilla Woolley Burton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Clarification of the bracteole restraint on germination and the development of an improved seed pre-treatment were the objectives of this study. Bracteole cell walls were isolated and hydrolyzed with 72% sulfuric acid followed by dilution and further hydrolysis. Individual monosaccharide yields were monitored with gas chromaixtography and colorimetry. Lignin was determined as the acid insoluble residue . Cell wall composition on a dry weight basis was 17.0% lignin, 32 . 75% glucose, 28.77% xylose, 7.21% arabinose, 1.11% galactose and 0.35% mannose. Linear xylans and glucans represent a large portion of the cell wall . The quantity of lignified tissue is …

Assay, Purification, And Characterization Of A Pantetheine Hydrolyzing Enzyme From Pig Kidney, Carl Thomas Wittwer

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A microsomal glycoprotein hydrolyzing pantetheine to pantothenate and cysteamine has been solubilized and purified to homogeneity as determined by sodium dodecylsulfate electrophoresis. Four rapid, independent assays of pantetheine hydrolysis are described and compared along with a method for localizing enzymatic activity on polyacrylamide gels. The enzyme is solubilized on exposure to butanol and purified by heat treatment, (NH4)₂SO₄ fractionation, hydrophobic chromatography, and hydroxyapatite chromatography. The glycoprotein, purified 5600-fold in 22% yield, has a specific activity of 14 μmoles pantothenate produced/min/mg of protein, 35 times that previously reported. The enzyme has a pH optimum of 9.0-9.5 and a …

Toxicologic Effects Of Extracts Of Cestrum Parqui L'Héritier In Mice, Tomás A. López

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cestrum parqui L'Héritier is a Solanaceous weed of wide distribution throughout the world. It causes poisoning of animals, particularly cattle and sheep, and constitutes a serious problem for animal production in some countries.

The present study was undertaken to develop a biological test for the detection of toxicity of extracts obtained from the plant. This test will be utilized to assess the toxicity of extracts obtained from the plant in future studies.

The plant is known to induce poisoning in laboratory animals, but there are no precise references on doses to be utilized and of appropriate routes of dosing. Oral …

Cougar Predation And Ecological Energetics In Southern Utah, Bruce Bennet Ackerman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Diet of cougars (Felis concolor) was studied from December 1978 to August 1981, on a 4500 km2 study area near Escalante, Utah. Prey eaten was determined from analysis of 112 animals consumed as prey, and from 239 cougar scats. Mule deer (Odocoileus hemionus) were the major prey item, comprising 81% of biomass consumed. Lagomorphs, large rodents, and smaller predators were also important components. Cattle comprised <1% of the diet, although commonly grazed on the cougar's summer range. Adult male and juvenile (<1yr) mule deer were killed more often than expected, in fall (P<0.10) and in winter (P<0.005). Cougars were a major cause of mortality of adult deer (41% of all adult female deaths, 35% of male), but only 6% of mortality of juveniles. Selection of prey seemed to be a function of prey vulnerability, rather than one of active choice by the predator.

Motion-sensitive radio-transmitters were placed on 15 cougars, from 3 months to 7-9 years of age. Three parameters of the radio signal were used to determine activity levels during 6843 …

Reproductive Behavioral And Physiological Traits In Domestic, Wild, And Hybrid Ovis, Kara-Lynn Crocker-Bedford

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was part of a program to develop new genotypes of sheep (Ovis spp.) and goats (Capra spp.) which are more useful for food and fiber production. The study examined the influence of domestication on behavioral and physiological traits of ewes and lambs, the influence of a single or twin offspring on ewe and lamb behaviors, and general relationships between ewes and lambs during the lambs' first month of life.

Domestication has caused the intensities of observed traits to diverge greatly from the tendencies shown by wild populations. Domestication has produced increases in measurements associated with maternal …

Comparison Of Growth In Preterm, Low-Birth-Weight Infants Fed Human Milk Versus Standard Infant Formula From 40-56 Weeks Postconceptual Age, Laurie Jean Moyer

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to compare growth following hospitalization in preterm, low birth weight infants fed their own mother's milk versus preterm, low birth weight infants of similar weight and gestational age given standard infant formula upon demand. Growth measurements were taken 40, 42, 48 and 56 weeks postconceptual age.

A total of 28 healthy, preterm, low birth weight infants completed the study. Seventeen infants received standard infant formula (Similac) and 11 were breast-fed upon demand. Introduction of solid foods was delayed until the infant was greater than 56 weeks postconceptual age. Weight, length, occiptial frontal circumference, mid-upper …

Associative Growth Of Paired Lactic Streptococci In Ph Controlled Whey-Based Media, Carol Lee Hansen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study evaluated four lactic streptococcal pairs which had been used routinely in cheese plants to determine (1) if strain growth interactions would produce stimulated growth and acid production; (2) final percent strain ratios and generation times after 1/1 initial inocula volumes and (3) whether final strain ratios could be controlled through adjustment of initial inocula volumes. Strains were propagated in fortified and unmodified whey substrate under pH control at 27C for 12 hours. Cell number and acid activity were evaluated on single and paired strains. Phage typing was used to determine generation times and the percent of each strain …

The Relationship Between Milk Composition And Swiss Cheese Yields, Gheyath H. Majeed

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Eighty-five samples of milk and Swiss cheese made from the same milk were collected at Cache Valley Dairy Association, Smithfield, Utah, between August, 1979, and July, 1980. The weights of the milk, Swiss cheese and the trim were carefully recorded. The milk samples were analyzed for fat and protein, and the cheese samples were analyzed for fat, protein and moisture. An attempt was made to predict Swiss cheese yields from the fat and protein content of the milk and the moisture content of the cheese.

The data were analyzed statistically by Gauss-Newton non-linear least squares method of iteration. Three formulas …

A Nutrition Education Componenet For Migrant Children Based On The K-6 Nutrient-Density Curriculum, Sandra Beadleson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A nutrition education component was developed, implemented, and evaluated for integration into the Title I migrant education program. The component was based on an evaluated nutrition education curriculum implemented by Brown in the elementary school. The nutrition education program was developed on the concept of nutrient density which measures the ratio of a food's nutrient contribution to its caloric contribution on a constant caloric basis. The nutrition component was modified to include behavioral objectives and concepts that partially met the needs of a migrant population in Utah.

The program was evaluated in the Title I programs in Corrine and Smithfield, …

Seasonal Variation In The Ability Of Milk And Whey To Support Lactic Culture Growth, Rick Cameron Norton

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Milk samples from two cheese plants with overlapping milk supplies were collected monthly for one year in an attempt to measure seasonal variation in the ability of milk and whey to support lactic culture growth . Treatments to control and minimize variability of milk or whey were evaluated to optimize stability in starter culture performance.

Raw milk samples were tested for somatic cell counts, activity tests (modified Horrall- Elliker), acid degree values, and total plate counts. Activity (modified Horraii-EIIiker) and inhibitory tests were also performed on pasteurized, pasteurized-vacuumized and high heat milk treatments.

Rennet whey (heated and unheated) was collected …

Prediction Of Mozzarella Cheese Yield From Milk Composition, Hamzah M. Abu-Tarboush

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was conducted to develop and evaluate several formulas which predict Mozzarella cheese yield from fat and protein content of milk and moisture content of cheese. During a one month period, 107 samples of milk and cheese were collected at Olympia Cheese Company, Olympia, Washington. Milk samples were analyzed for fat and protein content. Cheese samples were analyzed for fat, protein and moisture content.

Three models were derived to predict the yield of Mozzarella cheese. The three models were statistically fitted to the data by applying the Gauss-Newton non-linear least squares method of iteration. The differences among the three …

Variations In The Coagulation Properties Of Milk From Individual Cows, Leslie M. Okigbo

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Milk samples from fifty individual Holstein cows at the Utah State University Dairy Farm were tested monthly for 10 months for total protein, casein, fat, somatic cells and pH. A new instrument (Foss Formagraph) was used to measure the coagulation properties of the samples. Simulated cheese making procedures were utilized to measure recovery of fat and protein. All the data were statistically analyzed in relation to individual animals, stage of lactation, number of lactation and milk yield.

Significant variations in clotting time and curd firmness were observed in relation to period of lactation, season, individual cow difference, and milk pH. …

The Derivation And Validation Of A Workable Food Listing System For Use In Evaluation Of Food Consumption, Georgia C. Lauritzen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Three separate food grouping systems were derived for diet evaluation by utilizing a computer clustering program. System 1 was based on grouping of foods according to nutrient quality and quantity, system 2 was based on nutrient quality and frequency of consumption and system 3 was based on nutrient quality and kilocalories consumed. Defendable nutritional principles as well as knowledge of present nutritional concerns and eating habits of the U.S. population underpinned the derivation of the three systems. Over 4,000 individual foods were condensed to approximately 325 groups.

The three food grouping systems which were developed were compared for accuracy by …

A Revision Of The North American Species In The Genus Euodynerus (Hymenoptera: Eumenidae), Ali Shallan Moalif

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Twenty-eight species of Euodynerus Dalla Torre are recognized from the Nearctic Region, of which one is Holarctic. Literature on the biology of fourteen species is reviewed and new biological information discussed for four species. The phylogenetic relationships of the genus Euodynerus to other genera and the classification and relationships of the species of Euodynerus are discussed. Seven subgenera are recognized of which four are new: Euodynerus, Pareuodynerus Bluethgen, Leucodynerus Bohart, Annodynerus new subgenus, Gonodynerus n. subgenus, Holodynerus n. subgenus and Plecodynerus n. subgenus. Their comparative morphology, phylogeny, and distrubution are discussed. Larval instars of E. hidalgo (Saussure), E. scutellaris …

A Comparative Study Of Seizure Susceptibility And Serum Calcium, Magnesium And Phosphorus Profiles Of Magnesium Deficient Rats, Nicole H. Bernhard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Magnesium deficiency in rats is known to precipitate audiogenic seizures. An unknown mineral factor in a diet mixture was found to substantially reduce the seizure occurence in magnesium deficient vii animals. This was corrected when the same mineral mixture was remade. Subsequently the faulty mineral mixture was discarded. This research .. as aimed at determining the mineral factor responsible for the observed changes in seizure occurence, and also aimed at investigating the relationships of serum calcium, magnesium and phosphorus concentrations to seizure susceptibility. Treatments to change the serum concentrations of these minerals included dietary manipulation, subcutaneous injection of calcitonin, of …

Western White Pine: The Effect Of Clone And Cone Color On Attacks By The Mountain Pine Cone Beetle, Michael J. Jenkins

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The relationship between clone and cone color in western white pine, Pinus monticola Douglas, to attack by the mountain pine cone beetle, Conophthorus monticolae Hopkins, was studied in the Sandpoint Seed Orchard, Idaho. A positive relationship was shown to exist during a 5 year field evaluation. Cone beetles were found to prefer dark colored cones and to attack certain clones at a higher rate than others.

Laboratory dissections did not indicate that cone color affected oviposition, brood development or brood mortality.

Olfactometer experiments demonstrated that olfactory stimuli are involved in the cone beetle attack sequence. Visual cues relating to cone …

Plant Spacing: A Size Sensitive Model With Implications For Competition, Robert L. Bayn Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An algorithm is presented which partitions space among mapped plants according to their relative sizes and positions using one of eight rules for locating boundaries between individuals. The performance of those rules is examined using several natural and artificial data sets with diverse measures of individual size. The relative performance of the rules was the same for all natural data sets examined. The best rule, as measured by a high correlation between individual size and assigned space, placed the boundary at a distance between neighbors proportional to the relative sizes of neighbors as long as a maximum distance (also a …

Influence Of Photosynthetic Photon Flux Density On Uv-Binduced Photoinhibition In Soybean Leaves: Comparison Of Preconditioning And Concomitant Light Treatments, Charles W. Warner

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Previous studies indicate that the degree of UV-Binduced photosynthetic inhibition may be highly dependent upon the photosynthetic photon flux density (PPFD, total quantum flux in the waveband 400-700 nm) incident on a plant. This study illustrates that Essex soybean leaves (Glycine max) preconditioned under high PPFD suffered less UV-B-induced photoinhibition than when preconditioned under low PPFD. However, sensitivity to UV-B increased when soybean leaves received high-PPFD as a concomitant treatment.

The relative msgnitude of UV-B-induced damage was similar for both light-limited and light-saturated photosynthesis. This probably indicates that UV-B is inhibiting fundamentally different photosynthetic processes.

Soybean leaves preconditioned …

The Pathogenicity Of Blue-Stain Fungi On Lodgepole Pines Attacked By Mountain Pine Beetle, Richard Grant Ballard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In the western regions of North America, mountain pine beetle, Dendroctonus ponderosae Hopk., infestations take a tremendous toll of pines , especially lodgepole pine, Pinus contorta Dougl. var. latifolia Engelm.. Mass attack by the beetles is a devastating event for the trees. As well as girdling the tree, a massive inoculation of blue stain fungus "complex" (composed of several species of Ceratocystis, numerous yeasts and other mycelial fungi) is made beneath the bark. These fungi colonize and destroy the parenchyma tissue system of the host sapwood, primarily the ray parenchyma and resin duct epithelium.

A blue stain is produced …

Efficacy Of Denning In Alleviating Coyote Depredations Upon Domestic Sheep, James A. Till

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bands of domestic sheep lambing on the open range in south central Wyoming were monitored for predator losses prior to and following coyote (Canis latrans) removals. Experimental treatments, including 1) no removal (control), 2) removal of 2 adults and their pups, and 3) removal of pups only, were replicated 15 times each. Number of predation incidents (events) was reduced 98.2% by removing adults and pups. The number of sheep killed was reduced by 98.8%. Removing only litters of pups resulted in a decrease of 87.7% total kills decreased 91.6%. in predation incidents, while Overall, 23 of 30 predation …

Endocrine And Physiological Responses Of The Female Goat During Three Reproductive Phases, Dana Dean Clark

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Female Spanish x Dairy cross goats were divided into three reproductive phases--breeding season or cyclic (20), seasonal anestrus (20), and early postpartum (20). Each group of animals was further divided into three treatments--control (10), Gonadotropin Releasing Hormone (GnRH) treated (5), and pituitary Luteinizing Hormone (LH) (5). During each of the above mentioned reproductive phases, the following were measured: progesterone and LH profiles, serum LH levels following GnRH injection, pituitary LH concentration and ovarian responses following GnRH treatment.

Progesterone levels indicative of luteal development were present only in the breeding season. Progesterone levels during the seasonal anestrous and postpartum periods were …