Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Beef (3)
- Bobcat (2)
- GWAS (2)
- Lynx rufus (2)
- Microbiome (2)
-
- Multidrug resistance (2)
- QTL (2)
- 16S rRNA (1)
- 16S rRNA gene sequencing (1)
- ABC transporters (1)
- ATP-binding cassette transporters (1)
- Agrarian question of labor (1)
- Antioxidant (1)
- Auxin (1)
- Auxin biosynthesis (1)
- Auxin signaling (1)
- Bacillus amyloliquefaciens (1)
- Bacteria (1)
- Badlands National Park Bighorn Sheep Disease Population Genetics Survival (1)
- Beef producer (1)
- Biofuel Policy (1)
- Bioremediation (1)
- Biosensors (1)
- Bison (1)
- Body Composition (1)
- Botanical garden (1)
- Bradyrhizobia (1)
- Branched Chain Amino Acids (1)
- Bud break (1)
- Bud fruitfulness (1)
Articles 1 - 30 of 75
Full-Text Articles in Entire DC Network
Analysis Of Metabolites And Therapeutics For Toxic Inhaled Agent Exposure, Abigail Bemah Donkor
Analysis Of Metabolites And Therapeutics For Toxic Inhaled Agent Exposure, Abigail Bemah Donkor
Electronic Theses and Dissertations
The inhalation of toxic inhaled agents (TIAs) such as methyl isocyanate and cyanide can compromise an individual’s general health due to their extremely toxic nature. Nonetheless, these gases are used extensively in the electronics, chemical, welding, electroplating industries, etc., increasing the risk of exposure to both civilians and industrial workers. It is therefore very crucial to develop analytical methods for TIA metabolites and therapeutic agents. Methyl isocyanate (MIC), an intermediate in the synthesis of carbamate pesticides is a toxic industrial chemical that causes irritation and damage to the eyes, respiratory tracts, and skin. Due to its reactivity with proteins, protein …
Evaluating Impacts Of Tryptophan And Branched Chain Amino Acids In Swine Diets Containing Corn Based Dried Distillers Grains On The Growth Performance And Carcass Characteristics Of Grow-Finish Pigs, David Alan Clizer
Electronic Theses and Dissertations
A total of four studies were conducted in an effort to determine the impact of Trp and the BCAA in swine diets containing DDGS or HPDDG on the growth performance and carcass characteristics of the growing and finishing pig. The first study utilized 1,170 pigs (PIC 800 x PIC, initial BW 38.6 kg) in a 98-d grow-finish study to determine the performance response of pigs fed increasing levels of SID Trp:Lys in swine diets containing 40% DDGS. Treatments consisted of diets containing 40% DDGS with a SID Trp:Lys ratios of 15, 18, 21, or 24% or a corn-SBM diet for …
The Role Of The Auxin Biosynthesis And Signaling In Soybean Root Nodule Development, Mucahid Bozkus
The Role Of The Auxin Biosynthesis And Signaling In Soybean Root Nodule Development, Mucahid Bozkus
Electronic Theses and Dissertations
Nitrogen(N) is one of the most important plant nutrients for plant growth and yield, however, its abundance in the soil is not sufficient for profitable crop production. The use of chemical fertilizers helps address soil N deficiency in agriculture. However, due to the environmental pollution resulting from excessive use of fertilizers, alternative forms of N for agriculture are a necessity. Leguminous plants such as soybean (Glycine max) form a symbiotic association with N-fixing rhizobia to meet their N demands. Legume-rhizobia symbiosis results in the formation of unique structures called nodules where rhizobia convert atmospheric nitrogen into plant usable forms, thus …
Spatial And Temporal Patterns Of Sympatric Bobcats (Lynx Rufus) And Coyotes (Canis Latrans) In An Agricultural Landscape, Marlin M. Dart
Spatial And Temporal Patterns Of Sympatric Bobcats (Lynx Rufus) And Coyotes (Canis Latrans) In An Agricultural Landscape, Marlin M. Dart
Electronic Theses and Dissertations
Bobcat (Lynx rufus) populations experienced declines in the Midwest during the 20th century due to land conversion for agriculture and over exploitation and were practically nonexistent in areas by the 1970-80s. Populations have been recovering following changes in land-use practices and habitat improvement. Eastern South Dakota was closed to bobcat harvest in 1977 but reopened in 2012 to select counties. Bobcats are elusive, have large home ranges, and occur at low densities, making monitoring their populations difficult. Camera trapping is an effective tool for monitoring elusive carnivores but can be burdened by low detection rates. Researchers often employ …
Characterization Of The Rumen Bacterial Communities Of Bison Heifers Fed A Grass-Based Diet Vs A Grain-Based Free-Choice Diet, Anlly Miley Fresno Rueda
Characterization Of The Rumen Bacterial Communities Of Bison Heifers Fed A Grass-Based Diet Vs A Grain-Based Free-Choice Diet, Anlly Miley Fresno Rueda
Electronic Theses and Dissertations
A century ago, the North American grasslands and prairie ecosystems were dominated by bison. At least 30 million bison roamed the Great Plains when the first explorers arrived. By 1900, there were little over a thousand bison remained in the United States and Canada. Recovery efforts has been made since the 20th century to reestablish the herds and increase the bison population. Today, over 500,000 bison are distributed across North America, with more than 90% of the existing bison population under commercial production. Modern conservation strategies are made via the collaborative efforts of conservationist, producers, and researchers, resulting in increased …
Pheasant Ecology In An Agricultural Landscape Of South Dakota, Sprih Harsh
Pheasant Ecology In An Agricultural Landscape Of South Dakota, Sprih Harsh
Electronic Theses and Dissertations
Habitat loss and fragmentation are the greatest threats to wildlife conservation. Grasslands are among the most threatened ecosystems worldwide. The large-scale conversion of North American grasslands to cultivation has been strongly associated with declines of grassland bird populations. The ring-necked pheasant (Phasianus colchicus) is a common grassland bird which is negatively impacted by the conversion of grassland to cropland. Though pheasants are non-native to South Dakota, they have become naturalized in most of the state. However, with increases in agricultural intensification in South Dakota, indices of pheasant abundance from brood route surveys suggest that pheasant populations have declined to historically …
Interrogating The Socio-Ethical Dilemmas Of Precision Agriculture Technologies, Ayorinde Ogunyiola
Interrogating The Socio-Ethical Dilemmas Of Precision Agriculture Technologies, Ayorinde Ogunyiola
Electronic Theses and Dissertations
Farming has moved into a digital age where data and information are available to farmers to make informed agronomic and financial on-farm decisions. The development of precision agriculture (PA), such as big data technologies and machine learning algorithms is transforming the agricultural food production system in diverse ways, economically, environmentally, and socially. Despite benefits afforded by PA to agricultural productivity and environmental sustainability, these technologies can raise unintended societal challenges that can also limit their adoption among farmers. PA is changing how farming is done, reconstructing farmers’ social identities, and influencing relationships between farmers, agronomists, and technology developers. This dissertation …
Comparison Of Software Packages For Detecting Differentially Expressed Genes From Single-Sample Rna-Seq Data, Rong Zhou
Electronic Theses and Dissertations
RNA-sequencing (RNA-seq) has rapidly become the tool in many genome-wide transcriptomic studies. It provides a way to understand the RNA environment of cells in different physiological or pathological states to determine how cells respond to these changes. RNA-seq provides quantitative information about the abundance of different RNA species present in a given sample. If the difference or change observed in the read counts or expression level between two experimental conditions is statistically significant, the gene is declared as differentially expressed. A large number of methods for detecting differentially expressed genes (DEGs) with RNA-seq have been developed, such as the methods …
The Importance Of Care Coordination Of Patients With Multimorbidity: An Evaluation Of The Patient-Centered Medical Home Model, Mary Boksa
Biology and Microbiology Graduate Students Plan B Research Projects
The rise in multimorbidity among various patient populations has made fragmentation of care an increasingly common issue due to the need for specialized care of each chronic condition and complexity of treatment plans. Implementation of care coordination intervention models has been identified as an essential component in combating this fragmented nature of our current health care system. This is done by providing an organized model of care and improving communication between all parties involved in care and treatment plans. This literature review will begin with a brief discussion on the importance of care coordination and components needed for implementation and …
The Relationship Between Land Use Change And Disease Prevalence, Allison Bjornstad
The Relationship Between Land Use Change And Disease Prevalence, Allison Bjornstad
Biology and Microbiology Graduate Students Plan B Research Projects
Zoonotic disease outbreaks are becoming more novel and are increasing in frequency. This increase can be partially attributed to land use changes including deforestation, urbanization, and cultivation. Land use change alters disease prevalence by causing an increase in contact rates between disease hosts and humans. Land use changes also alter species compositions in the area to contain more competent disease hosts. Each pathogen can react to these changes in different ways. The specific reactions that each pathogen has to land use changes should be further researched so proper mitigation steps can be taken. Once the effects of land use change …
Polycystic Kidney Disease: An Examination And Review Of Disease Type, Presentation, Treatment, And Prognosis, Zoey Mann
Biology and Microbiology Graduate Students Plan B Research Projects
Polycystic Kidney Disease (PKD) is a ciliopathy that primarily presents as renal cysts. Inherited as either a dominant (ADPKD) or recessive (ARPKD) mutation, PKD is one of the most commonly inherited kidney diseases. ADPKD is caused by the inheritance of a mutation in either PKD1 or PKD2, which code for the polycystin-1 and -2 proteins, respectively. The less severe form of PKD, ADPKD is typically adult-onset, with the possibility of extremely late-stage presentation. In addition to renal cysts, hepatic and pancreatic cysts are common, as well as other non-cystic symptoms including headache and hypertension. ARPKD is caused by the inheritance …
Current And Future Perspectives In The Treatment Of Fracture Nonunions, Zebadiah Nelson
Current And Future Perspectives In The Treatment Of Fracture Nonunions, Zebadiah Nelson
Biology and Microbiology Graduate Students Plan B Research Projects
Fracture nonunions comprise one of the serious clinical complications in orthopedics. Fracture nonunions result when the time to union is greater than 6 to 9 months. It is estimated that approximately 5% to 10% of all fractures progress to fracture nonunion due to a host of complicating patient variables and risk factors. New scoring systems have emerged which help in directing the treatment strategies. Fracture nonunion management should be directed toward maintaining the diamond concept which introduces osteogenic cells lines, an osteoconductive scaffold for those cells to grow, as well as the necessary growth factors, and a stable mechanical microenvironment. …
Association Between Colorectal Cancer Prevalence And Patients Age, Race And Sex, Arsiema Fisuh
Association Between Colorectal Cancer Prevalence And Patients Age, Race And Sex, Arsiema Fisuh
Biology and Microbiology Graduate Students Plan B Research Projects
Colorectal cancer is one of the top leading causes of cancer deaths affecting people of different ages, sex and races disproportionately. The American Cancer society researchers have studied that lack of physical activities, smoking, consumption of red meat, and heavy alcohol use to be high risk factors for developing colorectal cancer in addition to genetic inheritances. Previous research studies have shown that disparities in cancer screening, socioeconomic status and others impacts on colorectal cancer prevalence and death. The objective of this project is to review the existing literature to better understand the disparities in colorectal cancer prevalence and deaths in …
Exploring Quantitative Trait Loci Mapping For Bud Fruitfulness And Bud Break Traits In Grapevine F2 Population, Seyma Bozkus
Exploring Quantitative Trait Loci Mapping For Bud Fruitfulness And Bud Break Traits In Grapevine F2 Population, Seyma Bozkus
Electronic Theses and Dissertations
Grapevine (Vitis vinifera L. subsp. vinifera) is one of the most important and valuable fruit crops around the world and grape industries in the USA have been growing increasingly regarding the demand for highly productive and quality grapes that can be grown in the cold region around the USA. The development of new cultivars with these features is performed through grape breeding with the help of quantitative trait loci (QTL) mapping, marker-assisted selection (MAS), and and other technologies. In this study, we evaluated grapevine bud fruitfulness by position and bud break in controlled conditions. These traits are multi-genetic and understanding …
Using New And Long-Term Multi-Scale Remotely Sensed Data To Detect Recurrent Fires And Quantify Their Relationship To Land Cover/Use In Indonesian Peatlands, Yenni Vetrita
Electronic Theses and Dissertations
Indonesia has committed to reducing its greenhouse gases emissions by 29% (potentially up to 41% with international assistance) by 2030. Achieving those targets requires many efforts but, in particular, controlling the fire problem in Indonesia’s peatlands is paramount, since it is unlikely to diminish on its own in the coming decades. This study was conducted in Sumatra and Kalimantan peatlands in Indonesia. Four MODIS-derived products (MCD45A1 collection 5.1, MCD64A1 (collection 5.1 and 6), FireCCI51) were initially assessed to explore long-term fire frequency and land use/cover change relationships. The results indicated the product(s) could only detect half of the fires accurately. …
Milk Phospholipids Extraction Using Switchable Solvents, Kaavya Rathnakumar
Milk Phospholipids Extraction Using Switchable Solvents, Kaavya Rathnakumar
Electronic Theses and Dissertations
Phospholipids (PLs) found in milk are of great interest due to their health and nutritional benefits associated with their consumption. By-products generated during the manufacture of dairy products represent a source of PLs with potential for value-added opportunities. Currently, the extraction of PLs from by-products results in overall lowefficiencies, and it involves subsequent solvent separation and lipid recovery. This dissertation studied the extraction of milk PLs from by-product streams with a tertiary anime (N, N-dimethyl cyclohexylamine, CyNMe2) as a switchable hydrophilicity solvent. This type of solvents can be reversibly switched between a hydrophobic and hydrophilic form in the presence or …
A Biocontrol Pesticide Derived From Mycovirus-Infected Sclerotinia Sclerotiorum Can Induce Plant Resistance, Connor Pedersen
A Biocontrol Pesticide Derived From Mycovirus-Infected Sclerotinia Sclerotiorum Can Induce Plant Resistance, Connor Pedersen
Electronic Theses and Dissertations
Soybean leaf-associated gemycircularvirus-1 (SlaGemV-1) is a novel mycovirus discovered through the metagenomic sequencing of soybean leaves which is capable of inducing hypovirulence in the highly pathogenic fungus Sclerotinia sclerotiorum. RNASeq analysis techniques were used to determine the transcriptional changes caused by the infection of virus in S. sclerotiorum, as well as the transcriptional changes in Glycine max caused by the colonization of hypovirulent, SlaGemV-1-infected S. sclerotiorum. RNASeq results indicate that viral infection leading to hypovirulence may attenuate expression of genes relating to cell wall synthesis, microtubule formation, and metabolism of steroids and natural antibiotics. Cytochrome P450-related genes, kinesin domain genes, …
Improving Nutritional Properties And Extractability Of Pea Proteins For Human Consumption Via Fungal Bioprocessing, Camille Massmann
Improving Nutritional Properties And Extractability Of Pea Proteins For Human Consumption Via Fungal Bioprocessing, Camille Massmann
Electronic Theses and Dissertations
Yellow peas are a rich source of protein and phytochemicals. Peas are compatible with many diets restricted by sensitivities, allergies, or personal choice. As a result, peas are gaining popularity in food markets, despite the challenges they present. The biggest challenges for pea proteins in food markets are high content of fiber, starch, and saponins. These components cause processing challenges and create undesirable textures and bitter flavors. Pea proteins are typically extracted with alkaline extraction or air classification methods which result in 80% and 50% protein products respectively. With low protein purity, these products are not competitive with other, more …
An Evaluation Of The Bighorn Sheep Population In Badlands National Park, Austin J. Wieseler
An Evaluation Of The Bighorn Sheep Population In Badlands National Park, Austin J. Wieseler
Electronic Theses and Dissertations
Within the last century, bighorn sheep (Ovis canadensis) in the badlands ecosystem of western South Dakota have been subjected to complete extirpation, reintroduction, disease die-offs, genetic bottlenecking, and population augmentation. Subsequently, the population in Badlands National Park (BNP) appears to have recovered, but it was unknown to what degree past events had influenced the population. From 2017-2019, we conducted research on 5 subherds within 2 management units in BNP to 1) survey for the presence of respiratory pathogens and estimate the prevalence of other potentially infectious diseases; 2) assess adult and lamb survival and cause-specific mortality; 3) estimate population size …
The Effects Of Weather, Body Weight, And Feed Intake On Total And Liquid Water Intake In Beef Cattle, Zachary Mcdaniel
The Effects Of Weather, Body Weight, And Feed Intake On Total And Liquid Water Intake In Beef Cattle, Zachary Mcdaniel
Electronic Theses and Dissertations
Two completely randomized design feedlot experiments were conducted over the course of a year. Both experiments were performed in order to determine the effects of weather, body weight (BW), and feed intake on total daily water intake (TDWI) and liquid daily water intake (LDWI) in beef steers. Experiment 1 utilized Angus weaned steers (n = 27; initial BW = 273 + 21.3 kg). Experiment 2 utilized yearling Bos taurus steers (n = 26; initial BW = 421 + 16.2 kg). Individual feed and water intake were collected using the Insentec (Insentec RIC, Hokofarm, Marknesse, Netherlands) automated feeding and water system. …
Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez
Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez
Electronic Theses and Dissertations
This Master thesis evaluates the impact of general health care and eating habits (Study 1), vision care (Study 2), and mental health status (Study 3) on Latino dairy farmworkers lifestyle and work performance in South Dakota. We hypothesized that the health status of dairy workers has a negative impact on the workers’ job performance. In study 1, data from a total of 70 participants were collected, using a face-to-face survey in Spanish, which allowed us to assess various topics and details related to employees’ daily routine tasks, eating habits, and general health status. Furthermore, recommendations to improve general health care …
Strength And Body Composition Changes Of Junior Hockey Players During The Competitive Season, Dan Marquette
Strength And Body Composition Changes Of Junior Hockey Players During The Competitive Season, Dan Marquette
Electronic Theses and Dissertations
Purpose: This study investigated the body composition and strength changes of NAHL junior hockey players across a competitive season. It was hypothesized that players would lose lean body mass loss from pre-season to end-season. The secondary hypothesis was that players would lose strength in relation to the anticipated loss in lean body mass. Methods: Outcome measurements were taken at pre-season, mid-season, and endseason points of a competitive NAHL junior hockey season (n = 15, age = 19.1 years). Body composition was measured using air plethysmography (BodPod) and strength was measured using isokinetic strength testing (Biodex). Results: No significant changes in …
Implications Of Broadleaf, Grass, And A Blend Of Broadleaf And Grass Cover Crops On Soil Health And Corn Production In South Dakota, Hunter Bielenberg
Implications Of Broadleaf, Grass, And A Blend Of Broadleaf And Grass Cover Crops On Soil Health And Corn Production In South Dakota, Hunter Bielenberg
Electronic Theses and Dissertations
Cover crops have recently gained attention in the U.S. Midwest because of their potential to increase soil organic matter and protect overall soil health. This study was conducted to determine the effects of different cover crop mixtures on soil health measurements and corn grain yield at increasing nitrogen (N) rates. Cover crops were planted in the fall after small grains harvest as a dominantly grass mixture, dominantly broadleaf mixture, or a 50/50 grass and broadleaf mixture with a no cover crop control. Soil and cover crop biomass samples were collected in the fall before winter cold termination and in the …
Soil Organic Carbon, Hydro-Physical Properties, Pore Structure And Greenhouse Gas Fluxes Under Integrated Crop-Livestock System, Jashanjeet Kaur Dhaliwal
Soil Organic Carbon, Hydro-Physical Properties, Pore Structure And Greenhouse Gas Fluxes Under Integrated Crop-Livestock System, Jashanjeet Kaur Dhaliwal
Electronic Theses and Dissertations
Integrated crop-livestock system (ICLS) can improve soil organic carbon (SOC), enhance soil hydro-physical properties, and mitigate greenhouse gas (GHG) emissions. The objectives of this study were to: (i) investigate the influence of grazing, cover crop (CC), and a combination of grazing and CC on labile soil carbon (C) and nitrogen (N) fractions and soil enzyme activity, (ii) assess the impacts of short-term (3-4 years) and long-term (>30 years) ICLS on SOC, bulk density, structural quality and water infiltration, (iii) evaluate soil pore structural properties such as porosity, number of pores, pore size, pore shape, fractal dimension, anisotropy and tortuosity …
Exploration Of Host Health Benefits By A Defined Consortium Of Butyrate-Producing Human Gut Bacteria In Gnotobiotic Mouse Model, Linto Antony
Electronic Theses and Dissertations
Aberrant gut microbiota composition is found to be associated with several human diseases such as inflammatory bowel diseases (IBD). Reduction in butyrate producing bacteria is one of the characteristic features of such dysbiotic bacterial community in the gut. Modulation of gut microbiota to bring the dysbiotic state back to normal healthy state is a promising therapeutic strategy to cure several diseases like recurrent Clostridium difficile infection (rCDI) and IBD where traditional therapies using pharmacological substances fails to make a difference. Restoration of butyrate producers is found to be an effective method of such gut microbiota modulation. The potential to produce …
Bio-Inspired Materials For Electrochemical Sensors, Matthew Joseph Hummel
Bio-Inspired Materials For Electrochemical Sensors, Matthew Joseph Hummel
Electronic Theses and Dissertations
Electrochemical biosensors are a rapidly growing research area that has greatly improved its specificity, accuracy, and precision in the detection of biomolecules in contemporary literature and industry alike. Typically, these systems exist in a three-electrode conformation with a working electrode functioning as the anode, a counter electrode functioning as the cathode, and a reference electrode allowing for the control of potential in the system. The method by which these sensors work is through the sharing of electrons via redox reactions with the target molecule and the working electrode or modifications on its surface. By exploiting the function of biomaterials that …
Management And Dietary Strategies To Mitigate Environmental Stressors In Northern Plains Cattle Feeding Systems, Warren Carl Rusche
Management And Dietary Strategies To Mitigate Environmental Stressors In Northern Plains Cattle Feeding Systems, Warren Carl Rusche
Electronic Theses and Dissertations
Three studies were undertaken to examine strategies to mitigate environmental stress faced by Northern Plains cattle feeders. In the first study, 46 feedlot managers from SD (n = 21), MN (n = 6), and NE (n = 19) were surveyed on use and perceptions of heat stress (HS) mitigation strategies. All respondents avoided cattle handling during heat stress events and incorporated some method of HS mitigation. Buildings or shades were perceived as the most successful strategy (60.9%) and were most commonly named as strategies managers would like to employ (63% for shades and 17.4% for buildings). Increasing dietary corn silage …
Evaluation Of Biotype 4 Aphis Glycines (Hemiptera: Aphididae) Induced Susceptibility On Soybean And Soja, Jakob David Hicks
Evaluation Of Biotype 4 Aphis Glycines (Hemiptera: Aphididae) Induced Susceptibility On Soybean And Soja, Jakob David Hicks
Electronic Theses and Dissertations
Soybean aphids have been a significant pest of soybeans in North America since 2000. Before 2000, soybeans did not face significant insect pest pressure from any arthropods with piercing-sucking mouthparts. It is estimated that economic damage from soybean aphids range from $1billion to $4.7 billion annually. Research efforts focused on the identification of host plant resistance genes in soybean and discovered many resistant to Aphis glycines genes (i.e., Rag genes) in soybean. However, the adoption of commercially released Rag soybean cultivars has been limited. The prospect of management with Rag genes was further complicated by the identification of three virulent …
Traditional And Local Food Procurement Motivators, Barriers, And Desires From Foodservice Directors In South Dakota Schools With High Enrollments Of Native American Students, Anna Barr
Electronic Theses and Dissertations
Purpose/Objectives: The objectives of the study were two-fold: 1) Determine the motivators and barriers to local food and local traditional food procurement among Food Service Directors (FSDs) of South Dakota (SD) schools serving Native American (NA) students, and 2) determine what traditional foods are desired for local procurement by the same population.
Methods: A cross-sectional survey design was used. An online survey was emailed to SD FSDs at 42 schools with high enrollments NA students. Eligibility was determined by 1) participation in National School Lunch Program (NSLP), and 2) >50% enrollment NA students. Motivators and barriers were determined by averaging …
Gene Network And Pathway Analysis Of Transcriptional Signatures Characterizing Sole Ulcer And Digital Dermatitis In Dairy Cows, Roshin Anie Mohan
Gene Network And Pathway Analysis Of Transcriptional Signatures Characterizing Sole Ulcer And Digital Dermatitis In Dairy Cows, Roshin Anie Mohan
Electronic Theses and Dissertations
This study aimed to evaluate the transcriptomic profile of both corium tissue from lactating dairy cows experiencing sole ulcers and skin lesions from lactating dairy cows diagnosed with digital dermatitis (DD). Hoof biopsies were performed in multiparous Holstein dairy cows selected based on their condition as clinically healthy or diagnosed with sole ulcers (n=7/group). Similarly, skin biopsies were taken from the center of active (M2/M4.1) DD lesions or non-active (M0/M4/M1) from multiparous Holstein dairy cows (n = 7/group) to assess the impact of DD on the skin transcriptome via RNA-seq analysis. All RNA samples were sequenced using Illumina, NovaSeq S4 …