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Immunization By Radiotherapy: Enhancing An Immune-Mediated Abscopal Effect, Amy Steffen
Immunization By Radiotherapy: Enhancing An Immune-Mediated Abscopal Effect, Amy Steffen
Biology and Microbiology Graduate Students Plan B Research Projects
Radiotherapy aims to destroy tumors by inducing DNA damage in their cells at the local irradiation site. Its immune-mediated systemic effects, called abscopal effect, has shown to enhance anti-tumor immunity when combined with immune therapies and thus, have gained attention by researchers and clinicians to investigate. Below is a summary of the fundamentals of cancer and the immune system’s response to it, as well as the changes in the phenotype and microenvironment of tumor cells after exposure to radiation. Impacts of the abscopal effect and the induction of effective antitumor immunity with various immune therapy strategies will be outlined. The …
Increasing The Clinical Efficacy Of Radiotherapeutics For Solid Tumors By Inhibition Of Hypoxia Inducible Factor-1a, Austin Levi Walz
Increasing The Clinical Efficacy Of Radiotherapeutics For Solid Tumors By Inhibition Of Hypoxia Inducible Factor-1a, Austin Levi Walz
Biology and Microbiology Graduate Students Plan B Research Projects
Radiotherapy is commonly used in a variety of tumor types and is effective in control of long term progression and may be curative under certain circumstances. Radiotherapeutics have been shown to be the single most effective therapeutic for cancer and only account for 5% of the total cost. However, treatment of cells with radiation causes the tumor microenvironment to undergo changes and leads to cycles of hypoxia inside the tumor which allows for the cell to undergo angiogenesis, metastasize and may lead to repopulation of the tumor. Current systemic chemotherapeutics typically have an additive toxicity due to the unintended targeting …
A Biological Review Of Mental Illness: An Overview Of Genetics And Pathophysiology Of Schizophrenia, Major Depression, And Addiction, Christopher R. Angerhofer
A Biological Review Of Mental Illness: An Overview Of Genetics And Pathophysiology Of Schizophrenia, Major Depression, And Addiction, Christopher R. Angerhofer
Biology and Microbiology Graduate Students Plan B Research Projects
Novel approaches in understanding mental illness present solutions to current issues in treatment and diagnosis. With advancements in genetics and neurology, the etiology and pathophysiology of mental illness is slowly unwrapping. In discovering biomarkers for depression, addiction, and schizophrenia, the precision for preventing, diagnosing, and treating these disorders increases. The aim of this paper is to review current research for addiction, major depression, and schizophrenia in genetics and physiology, while also clarifying the need for reclassifying mental illness as diseases with a pathophysiological basis, not syndromal, idiopathic disorders. Mental illness is dynamic in that it encompasses both environmental and biological …
Transcranial Magnetic Stimulaton: In-Depth Review Of Methods, Efficacy And Future Applications, Matthew Carpenter
Transcranial Magnetic Stimulaton: In-Depth Review Of Methods, Efficacy And Future Applications, Matthew Carpenter
Biology and Microbiology Graduate Students Plan B Research Projects
Transcranial magnetic stimulation (TMS) has been regarded as a novel technique of neuromodulation with applications that span from the treatment of depression or depression related symptoms to the mapping of cerebral regions. This review takes an in depth look into the different forms of magnetic stimulation including repetitive TMS, theta-burst stimulation, and navigated TMS. The efficacy of these different forms of stimulation is addressed as well as comparisons made to current standards being used. Potential clinically relevant future applications of TMS are also discussed from the treatment of obsessive compulsive disorder to improving motor function after stroke. The purpose of …
A Review Of Multiple Sclerosis Treatments: Interferon Beta, Glatiramer Acetate, Fingolimod, And Natalizumab, Ryan Golden
A Review Of Multiple Sclerosis Treatments: Interferon Beta, Glatiramer Acetate, Fingolimod, And Natalizumab, Ryan Golden
Biology and Microbiology Graduate Students Plan B Research Projects
Multiple Sclerosis (MS) is an autoimmune disease in which the body’s own immune system works to demyelinate the myelin sheaths in the central nervous system. The beginning of the review will concentrate on the history of MS while also describing how MS, the disease, differs from that of normal immune responses. The review then shifts to the four different stages of MS and how to differentiate between the stages, since the treatment options are often dependent on the stage of MS that the patient is in. The main focus of this review is to take an in depth look on …
The Influence Of Spore-Forming Microorganisms On The Quality And Functionality Of Cultured Dairy Products, Dipakkumar Mehta
The Influence Of Spore-Forming Microorganisms On The Quality And Functionality Of Cultured Dairy Products, Dipakkumar Mehta
Electronic Theses and Dissertations
Spore formers are observed as the common spoilage-causing microflora in milk and dairy products. Their spore-forming ability increases their survivability during heat treatment which leads to their presence in a final product. Spore-forming bacteria have ability to produce many types of spoilage-causing enzymes which are potential to influence quality and functionality of the final product. Many researchers have studied the ability of spore formers to induce a spoilage in various dairy products but as per our knowledge, their spoilage-causing activities are not described in a detail for cultured dairy products. In our first objective, we have studied the ability of …
Tripartite Interactions Of Legumes With Arbuscular Mycorrhizal Fungi And Rhizobial Bacteria: Insight Into Plant Growth, Seed Yield, And Resource Exchange, Arjun Kafle
Electronic Theses and Dissertations
Under natural conditions, legumes, such as alfalfa (Medicago) and soybean (Glycine max) are colonized with arbuscular mycorrhizal (AM) fungi and rhizobial bacteria forming tripartite interactions. Legumes are important crop species due to their high nutritional and economic values. Most of the previous literatures focused on experiments with an individual symbiont: either AM fungi or rhizobial bacteria, but not with both symbionts at the same time, thus our current understanding of resource exchange in tripartite interactions is limited. It has been reported that AM fungi primarily provide phosphate (P), nitrogen (N), and other nutritional and non-nutritional benefits while rhizobial bacteria solely …
Development Of Vegetation Mapping With Deep Convolutional Neural Network, Sae-Han Suh
Development Of Vegetation Mapping With Deep Convolutional Neural Network, Sae-Han Suh
Electronic Theses and Dissertations
The Precision Agriculture plays a crucial part in the agricultural industry about improving the decision-making process. It aims to optimally allocate the resources to maintain the sustainable productivity of farmland and reduce the use of chemical compounds. [17] However, the on-site inspection of vegetations often falls to researchers’ trained eye and experience, when it deals with the identification of the non-crop vegetations. Deep Convolution Neural Network (CNN) can be deployed to mitigate the cost of manual classification. Although CNN outperforms the other traditional classifiers, such as Support Vector Machine, it is still in question whether CNN can be deployable in …
Gtpase-Activating Protein-Binding Protein 1 Plays An Antiviral Role In Porcine Epidemic Diarrhea Virus (Pedv) Replication, Kabita Pandey
Gtpase-Activating Protein-Binding Protein 1 Plays An Antiviral Role In Porcine Epidemic Diarrhea Virus (Pedv) Replication, Kabita Pandey
Electronic Theses and Dissertations
Porcine Epidemic Diarrhea Virus (PEDV) is a non-segmented virus which uses one-stranded, positive-sense RNA as genetic material. PEDV falls under Coronaviridae family and extensive diarrhea and dehydration in nursing piglets are the major clinical signs. This emerging disease leads to huge economic loss in the pig farming. Very less studies are done towards the role of innate immunity in PEDV infection. The formation of Stress granules (SGs) are seen when cells are introduced to different stressful conditions, which also includes viral infections. SGs are formed when one of the four kinases: doublestranded RNA (dsRNA)-dependent protein kinase (PKR), PKR-like endoplasmic reticulum …
Surveillance Of South Dakota Mosquito Abundance, Infection Rate, And Insecticide Susceptibility, Geoffrey P. Vincent
Surveillance Of South Dakota Mosquito Abundance, Infection Rate, And Insecticide Susceptibility, Geoffrey P. Vincent
Electronic Theses and Dissertations
The aim of this dissertation was to survey and evaluate the abundance, infection rate, and insecticide susceptibility of mosquito fauna present in South Dakota. Mosquito surveillance has been conducted across South Dakota to record and track potential West Nile virus (WNV) vectors from 2004 to 2017. The nuisance mosquito Aedes vexans was found to be the most abundant species overall in the state and most abundant in many of the regions. The WNV vector, Culex tarsalis, was found to be the second most abundant mosquito and the most abundant vector mosquito across the state. However, geospatial variation did exist between …
Map-Based Cloning And Molecular Characterization Of The Seed Dormancy 10 Locus In Rice (Oryza Sativa L.), Wirat Pipatpongpinyo
Map-Based Cloning And Molecular Characterization Of The Seed Dormancy 10 Locus In Rice (Oryza Sativa L.), Wirat Pipatpongpinyo
Electronic Theses and Dissertations
Seed dormancy is a key adaptive trait of both ecological and agricultural importance. Although many quantitative trait loci (QTL) have been associated with seed dormancy in cereal crops or model plants, a majority of them remain unknown for molecular identities and functions. Cereal crops and wild/weedy relatives usually have weak and strong seed dormancy, respectively, such as Asian cultivated rice vs. weedy rice (Oryza sativa L.). Previous research identified a set of seed dormancy QTL, including qSD10, in single plant-derived BC1F2 and BC1F3 populations from the backcross between the weedy rice line SS18-2 …
Dissecting Rna Silencing Pathways In Sclerotinia Sclerotiorum, Pauline Mochama
Dissecting Rna Silencing Pathways In Sclerotinia Sclerotiorum, Pauline Mochama
Electronic Theses and Dissertations
RNA silencing, also known as RNA interference, is an essential mechanism in plants, animals and fungi that functions in gene regulation and defense against foreign nucleic acids. In fungi, RNA silencing has been shown to function primarily in defense against invasive nucleic acids. RNA-silencing- deficient fungi show increased susceptibility to virus infection. Plant pathogenic fungi also utilize RNA silencing to silence plant host immunity genes through the delivery of fungal small RNAs into plants. This cross-kingdom RNA silencing facilitates fungal infection of plants. Overall, these findings demonstrate the significant contributions of fungal RNA silencing pathways to fungal virulence and viral …
Vulnerability Of Protected Areas To Human Encroachment, Climate Change And Fire In The Fragmented Tropical Forests Of West Africa, Francis Kwabena Dwomoh
Vulnerability Of Protected Areas To Human Encroachment, Climate Change And Fire In The Fragmented Tropical Forests Of West Africa, Francis Kwabena Dwomoh
Electronic Theses and Dissertations
The Upper Guinean region of West Africa is home to some of the most globally significant tropical biodiversity hotspots, providing ecosystem services that are crucial for the region’s socio-economic and environmental wellbeing. Nonetheless, following decades of human-caused destruction of natural habitats, protected areas currently remain the only significant refugia of original vegetation relics in landscapes that are highly fragmented. Aside from having strong geographic variation in land use, climate, vegetation, and human population, the region has also experienced remarkable biophysical and socio-economic changes in recent decades. All these factors influence the fire regime and the vulnerability of forests within protected …
Regulation Of Local Auxin Metabolism During Soybean Nodule Development, Suresh Damodaran
Regulation Of Local Auxin Metabolism During Soybean Nodule Development, Suresh Damodaran
Electronic Theses and Dissertations
Legume-rhizobia symbiosis leads to the development of secondary root organs called nodules. Rhizobia bacteria housed inside nodules assimilate atmospheric nitrogen and convert them into plant usable forms thereby reducing the need for fertilizer application in crop legumes like soybean. Nodule development is a coordinated process orchestrated by multiple plant hormones. In soybean, the auxin responsive gene expression was detected in nodule primordia and in the periphery of mature nodules, primarily in nodule vasculature. Auxin hypersensitivity reduces nodule formation in soybean and also polar auxin transport inhibition at the site of nodule development is not crucial for determinate nodule formation. Therefore, …
The Role Of Genomic Versatility In Multi-Niche Preferences Of Escherichia Coli, Gitanjali Nandakafle
The Role Of Genomic Versatility In Multi-Niche Preferences Of Escherichia Coli, Gitanjali Nandakafle
Electronic Theses and Dissertations
Escherichia coli strains are naturally present as either commensals or pathogens in the gastrointestinal tract of mammals and some other vertebrates. Until recently, it was assumed that E. coli are solely associated with the gut and are unable to survive outside of a host for a long period of time, the basis of its use as an indicator organism. Recent reports suggest that E. coli can become naturalized to several tropical, subtropical or temperate soils and aquatic environments, where they have been isolated repeatedly. Several studies have shown that these strains are capable of surviving and proliferating in the environment …
Proteogenomic Study Of Nitrogen-Fixing Cyanobacterium Anabaena Cylindrica, Yeyan Qiu
Proteogenomic Study Of Nitrogen-Fixing Cyanobacterium Anabaena Cylindrica, Yeyan Qiu
Electronic Theses and Dissertations
Cyanobacteria are gram-negative photosynthetic bacteria. In some filamentous cyanobacteria such as Anabaena cylindrica, every 10th to 20th vegetative cells can differentiate into nitrogen fixing heterocysts. Heterocyst can induce the neighboring cells developing into spore-like akinetes. The specialized cell functions and resourceful networks have contributed to the prosperity of cyanobacteria for over 2 billion years, but the genetic mechanisms for multi-cellular differentiation are barely known, especially for akinete formation. The heterocysts, akinetes and vegetative cells of Anabaena cylindrica were isolated for proteomic study. This study identified a total of 1395 proteins, including 664 proteins from akinetes, 751 proteins from …
Effect Of Exogenous Amylase And Protease On Ruminal Metabolism, Nutrient Digestibility, Rumen Microbiome, And Lactation Performance Of Dairy Cows Fed Freshly Ensiled Corn Silage Based Diets, Lauren Kaye Shearer
Effect Of Exogenous Amylase And Protease On Ruminal Metabolism, Nutrient Digestibility, Rumen Microbiome, And Lactation Performance Of Dairy Cows Fed Freshly Ensiled Corn Silage Based Diets, Lauren Kaye Shearer
Electronic Theses and Dissertations
The objective of this study was to examine the effects of feeding exogenous amylolytic and proteolytic enzymes on lactation performance of dairy cows fed freshly ensiled corn silage-based diets. We hypothesized that the addition of these enzymes would improve nutrient utilization and consequently lactation performance. Thirty-six Holstein cows [15 multiparous and 15 primiparous; DIM = 132 ± 48 and 6 cannulated (3 multiparous and 3 primiparous; DIM = 164 ± 50] were blocked by milk yield, DIM, parity, and body weights and used in a 9 wk randomized complete block design study.. Treatments were a 40% (DM basis) corn silage …
Strategies To Improve Calf Performance: Improving Water Quality, Feeding Microbially-Enhanced Soy Protein, And Supplementing Condensed Whey Solubles, Nirosh Dias Senevirathne
Strategies To Improve Calf Performance: Improving Water Quality, Feeding Microbially-Enhanced Soy Protein, And Supplementing Condensed Whey Solubles, Nirosh Dias Senevirathne
Electronic Theses and Dissertations
Six experiments were conducted for this dissertation research to evaluate strategies to improve growth performance and health of calves. Growth performance, nutrient utilization, and health effects were evaluated when calves were offered water treated with reverse osmosis system (RW), fed starter pellets supplemented with microbially-enhanced soy protein (MSP) or supplemented condensed whey solubles (CWS). The first two experiments were conducted to determine drinking preference, growth performance, and health of dairy calves offered RW or by a municipal water treatment plant (MW), compared with local untreated well water (WW). First a sequential elimination study was conducted to observed drinking preference of …
Measuring The Influence Of Bedding On Soil Nitrogen Losses And Corn Crop Nitrogen Characteristics For Fall Applied Solid Beef Cattle Manure In Eastern South Dakota, Mukesh Mehata
Electronic Theses and Dissertations
Nitrogen (N) is a major component of chlorophyll which plays a key role in the photosynthesis process in crops. The N is one of the highest demanded nutrients by all plants for their growth and reproduction. Manure or inorganic fertilizer is often applied to fulfill the crops’ N demand. However, the applied N sources have the potential of N losses in different forms from the soil volume in many ways such as ammonia (NH3) volatilization, aerial nitrous oxide (N2O) loss, nitrate (NO3 --N) leaching, and runoff and/or erosion. Soil fertility, crop yield, water quality, and …
Universal Swine Influenza Antigen And Vaccine, Zhao Wang
Universal Swine Influenza Antigen And Vaccine, Zhao Wang
Electronic Theses and Dissertations
Swine influenza A viruses pose a serious concern for global health and worldwide swine industry considering their evasion from vaccine-induced immune responses. Formulation of effective influenza vaccines can be complicated by both antigenic shift to a different subtype of hemagglutinin (HAs) and antigenic drift within a particular HA subtype. To address these concerns, we employed a systematic approach to determine the antigenic determinants of swine influenza A viruses by focusing on H1 and H3 subtypes. In parallel, we used a cross-disciplinary approach including structural biology and modeling, virology, and immunology to identify swine influenza A virus-derived HA fusion intermediates broadly …
Differences In Walking Mechanics Between A Traditional Walker And The Kb Balance Trainer, Silvia Zanini
Differences In Walking Mechanics Between A Traditional Walker And The Kb Balance Trainer, Silvia Zanini
Electronic Theses and Dissertations
INTRODUCTION: Millions of individuals with ambulatory difficulties rely on walking aids to maintain independence and mobility. However, users of traditional walkers typically exhibit increased forward lean of their trunk while using the assistive device. The KB Balance Trainer is a new posterior walker designed to facilitate a more erect position during gait. PURPOSE: To compare gait mechanics across three walking conditions: unassisted, using a traditional walker, and using the KB Balance Trainer. METHODS: Seven adults with experience using walkers due to ambulatory difficulties participated in the study. The study consisted of one training session and one gait analysis session. The …
Impacts Of Diverse Crop Rotations And Integrated Crop-Livestock System On Soil Quality In South Dakota, Atilla Polat
Impacts Of Diverse Crop Rotations And Integrated Crop-Livestock System On Soil Quality In South Dakota, Atilla Polat
Electronic Theses and Dissertations
Crop diversification can be beneficial in improving soil quality parameters. Diverse crop rotations and integrated crop-livestock systems (ICLS), are two examples of crop diversification used in this study to assess soil quality. The objectives of this study were to: (i) assess the impacts of diverse rotations on soil quality parameters, and (ii) assess the impacts of low and high stocking density on soil quality parameters. Both of the studies were conducted separately and are presented as two different chapters. To accomplish objective (i), a long-term experiment was selected which was established near Brookings, South Dakota, USA at USDA-ARS lab in …
Mild Traumatic Brain Injuries And Cte: A Review Highlighting The Connection Between The Two, Leandre Kennedy
Mild Traumatic Brain Injuries And Cte: A Review Highlighting The Connection Between The Two, Leandre Kennedy
Biology and Microbiology Graduate Students Plan B Research Projects
Chronic Traumatic Encephalopathy (CTE) is a traumatic brain injury based disease that is garnering a lot of attention in the United States. The purpose of this paper is to conduct an extensive review of the information available about the condition. Recently there has been a limited amount of published information and treatment opportunities are rare since the only way of diagnosing has come post mortem. Various literary reviews and experiments were analyzed for appropriate information regarding CTE’s pathology and treatments and only peer reviewed published works were allowed in this paper. This resulted in promising information about the disease and …
Influence Of Production System On Animal Performance, Carcass Characteristics, Meat Quality, Environmental Impacts, Production Economics, And Consumer Preference For Beef, Megan Jean Webb
Electronic Theses and Dissertations
The overall objective of this study was to determine if the level of growth promotant technology used among production systems influence animal and carcass performance, meat quality, production economics, the environmental impact, and determine consumer preferences and perception. Angus � Simmental steer calves (n =120) were stratified by birth date, birth weight, and dam age in a completely randomized design and assigned to one of four treatments: 1) no antibiotics (NA, receiving no technology); 2) non-hormone treated (NHTC, fed monensin and tylosin); 3) implant (IMPL, administered a series of three implants), and 4) implant plus fed a beta-agonist (IMBA, administered …
Impacts On Salmonid Rearing Performance With Use Of Dietary Bioprocessed Plant-Based Protein And Water Velocity, Jill Voorhees
Impacts On Salmonid Rearing Performance With Use Of Dietary Bioprocessed Plant-Based Protein And Water Velocity, Jill Voorhees
Electronic Theses and Dissertations
The inclusion of bioprocessed soybean (Glycine max) meal was evaluated in five experiments using either Rainbow Trout (Oncorhynchus mykiss) or Brown Trout (Salmo trutta). In the first experiment, adult Erwin x Arlee strain Rainbow Trout were fed diets formulated so that bioprocessed soybean meal (BSM) replaced 0, 60, or 80% of the dietary fishmeal. There were no significant differences in gain, percent gain, feed conversion ratio, specific growth rate, intestinal morphology, relative fin lengths, or organosomatic indices. In the second experiment, juvenile Plymouth strain Brown Trout were fed diets formulated so that BSM replaced 0, 60, 80, or 100% of …
A Comprehensive Analysis Of Aromatic-Proton Mediated Hydrogen Bonds, Mona S. Alshamrani
A Comprehensive Analysis Of Aromatic-Proton Mediated Hydrogen Bonds, Mona S. Alshamrani
Electronic Theses and Dissertations
Hydrogen bonds play critical role in folding, structure and recognition of biological macromolecules (e.g., proteins, RNA, DNA). In addition to classical hydrogen bonds (e.g., ─OH---O=, ─OH---O─, ─NH---O─ etc.), structural analysis of protein and nucleic acids, almost a decade ago, showed that hydrogen bonds (e.g., CH---O) with hydrogen atoms on aliphatic carbon atoms (hereafter, aliphatic-protons) also play very important role in the structure and function of biomolecules. Even though, protons of aromatic ring systems (hereafter, aromatic-protons) are more polar than the aliphatic-protons, systematic analysis of hydrogen bonds of aromatic-protons have not been carried out. Therefore, I carried out a systematic analysis …
A Pcr/Sequencing Technique To Identify Species And Genotypes Of Haemonchus From Mixed Trichostrongyle Egg Dna Recovered From Livestock Fecal Samples, Pratiksha Khanal
A Pcr/Sequencing Technique To Identify Species And Genotypes Of Haemonchus From Mixed Trichostrongyle Egg Dna Recovered From Livestock Fecal Samples, Pratiksha Khanal
Electronic Theses and Dissertations
Six primer pairs, each with a pair of genus-specific and universal second internal transcribed spacer (ITS2) primers were evaluated to amplify and sequence the ITS2 region of nuclear ribosomal DNA to identify the species and genotypes of Haemonchus from livestock fecal samples containing mixtures of trichostrongyle nematode eggs. Based upon the use of universal ITS2 primers on Haemonchus adults, the ITS2 region has been shown to contain three fixed single-nucleotide differences between H. placei and H. contortus. It is clinically useful to identify the species and genotypes of Haemonchus infecting livestock, but it has not been possible in fecal …
Weight Management Related Factors And Healthy Behaviors In Adults, Sumadhuri Pamarthi
Weight Management Related Factors And Healthy Behaviors In Adults, Sumadhuri Pamarthi
Electronic Theses and Dissertations
Obesity is an ongoing global pandemic that affects more than one in three adults in the United States. Being overweight or obese also increases the risk of chronic health conditions. Weight management does not only center on immediate weight loss but also on the proper maintenance of an appropriate body weight over the years. Methods of weight management include eating a healthy diet and meeting physical activity guidelines. Such weight management practices are important to ensure that the people suffering from obesity can decrease risks of morbidity or even mortality. Nevertheless, unhealthy weight management strategies might lead to unintended health …
Identification Of Macropinocytosis Regulating Proteins And Signaling From Macropinosomes, Louise Monga
Identification Of Macropinocytosis Regulating Proteins And Signaling From Macropinosomes, Louise Monga
Electronic Theses and Dissertations
Understanding macrophage cell biology is important due to macrophages key roles in human health and diseases including proper immune function, wound healing, atherosclerosis, and cancer. Despite their importance, relatively little is understood about macrophage activation, growth factor signaling, and cytoskeletal regulation. This thesis presents data from investigations into mechanisms of macrophage growth factor signaling and actin polymerization for ruffling and macropinocytosis. Alt-R CRISPR-Cas9 method and dextran uptake assay were used to knock out individual genes (SHP-1, Lyn, Syk, BTK, Vav1) and determine their role in macropinocytosis and CSF-1R signaling. Dextran uptake was disrupted in SHP-1 and Lyn targeted knockout cells, …
Transcriptome Analysis Of Root Development In Wheat Triticum Aestivum Using High Throughtput Sequencing Technologies, Ghana Challa
Transcriptome Analysis Of Root Development In Wheat Triticum Aestivum Using High Throughtput Sequencing Technologies, Ghana Challa
Electronic Theses and Dissertations
Root provides plant water, nutrients and anchorage from soil. Most our knowledge of molecular mechanisms of root development is from the dicot model plant Arabidopsis, but very few studies have done in monocot crop systems like rice, maize, and wheat. We are studying very short root (VSR) phenotype in wheat, and lack of a sequenced reference genome in wheat prompted us to sequence and assemble the root transcriptome of the reference cultivar Chinese Spring (CS). A root transcriptome was assembled from the sequenced reads generated from root tip and the mature root tissues of CS. Approximately 169 million reads were …