Digital Commons Network^™

Developing Methods To Survey, Collect, Process, And Screen Endemic Entomopathogenic Fungi Against The Asian Citrus Psyllid In The Lower Rio Grande Valley, Jonathan Cisneros

Theses and Dissertations

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), vectors “Candidatus Liberibacter spp.”, the causative agent of Citrus Greening Disease. Managing ACP is imperative given the continuous increase in HLB positive trees. An aspect of integrated pest management (IPM) is the use of entomopathogenic fungi for the biological control of D. citri.

In order to identify endemic entomopathogenic fungi actively infecting D. citri, ACP were collected from local residential areas, surface sterilized, and plated on a semi-selective agar medium. Collection of over 9,300 samples from 278 sites throughout the LRGV led to the positive identification of …

Native Plant Allelopathy: A Potential Approach To Limit Invasive Grass Encroachment In Thorn Forest Restoration, Emily A. Mullins

Theses and Dissertations

Less than 5% of the Tamaulipan thorn forest remains in the United States. For this reason, there have been many attempts at restoration of this ecosystem. Oftentimes these attempts are unsuccessful due to the high prevalence of invasive African grasses that outcompete the seedlings. In an effort to improve the efficacy of these restoration efforts, native plant allelopathy has been examined for its ability to exclude invasive guineagrass. Native species were surveyed and selected species were tested for allelopathy in laboratory bioassays. The species that exhibited allelopathy in the laboratory were then evaluated in pot experiments for their ability to …

Discerning Competitive Vs. Facilitative Relationships Of Weeds On Forest Restoration Efforts At La Sal Del Rey, Tx, Mylen A. Arias

Theses and Dissertations

Dryland ecosystems need effective restoration strategies to address severe degradation. It is often assumed that voluntary forb weeds either compete with or have no effect on native seedlings. In contrast, theory and empirical work suggest a potential facilitative role for such forb weeds. We conducted a manipulative weed exclusion experiment at a semi-arid site in South Texas targeted for large scale forest restoration and subsequently dominated by early successional forb weeds to discern the net effect of these weeds on the growth and survival of target thornscrub tree and shrub seedlings. Overall, weed presence did not significantly affect seedling mortality …

Incorporation Of Native Plants For Biodiversity Conservation In South Texas Agroecosystems, Kaitlynn M. Lavallee

Theses and Dissertations

I address the concerning disconnect between food production and regenerative ecological principles. First, understanding the foundational processes of seed-dormancy and germination are essential for successful restoration efforts using native species. We examine four common seed treatments (aerated hydroprime, acid scarification, cold stratification, sand scarification) on twelve commercially available species native to south Texas. Pappophorum bicolor Fourn. (pink pappusgrass) was sown in the field with the aerated hydroprime treatment, D. virgatus (Willd.) B.L. Turner (prostrate bundleflower) was planted after sand scarification treatment, and Ratibida columnifera (Nutt.) Woot. & Standl. (Mexican hat) was seeded without treatment. Small-scale field trials were conducted to …

Exploring Plant-Plant Interactions And Nutrient Manipulation As Strategies For Thorn Scrub And Thorn Forest Restoration, Edgar D. Vasquez

Theses and Dissertations

Restoration of thorn scrub and thorn forest communities in South Texas is hindered by harsh environmental conditions and hypercompetitive invasive grasses. The success of thorn scrub/thorn forest restoration efforts depends on increasing seedling survival and growth by reducing stress after transplantation. Four experiments evaluated initial effects of plant-plant interactions and nutrient manipulation on thorn forest restoration. High density planting along with the use of nurse plants look promising; survival was generally higher under these conditions and growth was unaffected or in the case of high-density planting, promoted. Nutrient sequestration by sorghum had little effect on seedling performance and cover of …

Examining The Efficacy Of Cover Crops As An Integrated Pest Management Tool In Organic Farms In The Lower Rio Grande Valley, Lili M. Martinez

Theses and Dissertations

The Lower Rio Grande Valley (LRGV) consists of a mix in subtropical and costal habitats, a combination that provides a favorable environment for the animals and plants that inhabit it. This is a problem for organic farmers as they refrain from using synthetic chemicals and therefore led them to seek other methods of dealing with insect herbivory. Of the many alternative methods available, we looked specifically at integrated pest management methods (IPM) to determine their efficacy in reducing insect herbivory among cash crops. Chapter II discusses an experiment performed in an organic farm in Edinburg, Texas consisting of a two-year …

Aquatic Stage Mosquito Biocontrol: Effects Of Entomopathogenic Fungi Blastospores And Conidia On Aedes Aegypti Pupae, Ricardo Alberto Ramirez Garcia Rojas

Theses and Dissertations

Fungal entomopathogens present an opportunity to produce mosquito controls on-site, from remote locations to developing communities. Locally produced biopesticides have the potential to empower communities to develop internal disease vector control initiatives to protect local public health. We tested the effectiveness of the conidia solution and blastospore solution of Metarhizium anisopliae E9, Metarhizium anisopliae E1037, Isaria fumosorosea A97, and Isaria fumosorosea 9901 in controlling Aedes aegypti pupae. We observed that entomopathogenic fungi liquid blastospore solution induce mortality in Aedes aegypti pupae at a significantly greater rate than conidia solution and control treatment.

However, further analysis of testing methods used in …

The Application Of Advanced Technologies For Agriculture And Rangeland Management, Matthew D. Kutugata

Theses and Dissertations

This project demonstrates two applications of remote sensing in agricultural and rangeland environments. In the first, an unmanned aerial system (UAS) equipped with a multi-spectral sensor was used to estimate canopy cover across four different cover crop trials at four time periods. In the second, a local database of stationary camera trap images of wildlife was used to train a convolutional neural network to automatically catalogue images by identifying the animal in those images. Both projects aimed to provide an example of how remote sensing platforms and machine learning techniques can facilitate the rapid collection and processing of large-scale field …

Building Food Democracy In The Rio Grande Valley: Exploring The Relationship Between Civic Agriculture And Civic Engagement, Allison P. Kaika

Theses and Dissertations

Civic engagement is an important indicator of social capital in a community. The foundation of a strong democracy is dependent on citizens willingness and ability to engage. To further understand these drivers of social network building, this study utilizes the theory of civic agriculture to measure the impact of food procurement systems on civic engagement. A survey of over 400 residents in the Lower Rio Grande Valley of South Texas measures how involvement in local food systems impacts a participants’ contribution to and perception of his or her community, while considering important third factor variables that also influence food procurement …

Comparing Outdoor Vertical Hydroponic Farming Systems And Conventional Hydroponic Farming Systems With A Focus On Efficiency, Itohan A. Edokpolo

Theses and Dissertations

Combining hydroponics and vertical farming creates unique growing system that reduces the need for excessive water and land usage to grow more food. The main critique of this system is the high cost of setup, run and the potential drawbacks of shading on plant productivity. Five species were grown in three hydroponic designs known as horizontal, vertical and a-frame. The designs were compared in terms of productivity, space and energy. Results showed variability in plant productivity in the designs can be attributed to other factors other than light. Differences in species performance were seen in response to different factors. Horizontal …

Comparing Novel And Traditional Sampling Methodologies To Analyze The Population Status Of The Rio Grande Cooter (Pseudemys Gorzugi), Amy P. Bogolin

Theses and Dissertations

The Rio Grande Cooter, Pseudemys gorzugi, is an understudied aquatic turtle species of conservation concern in the Rio Grande, Pecos, and Devils river systems. Traditional sampling methodologies for aquatic turtle species face numerous challenges, but novel sampling methodologies, such as drone-based surveys and environmental DNA analysis, may address these issues. This study compared novel sampling methodologies to traditional sampling methodologies in mean detections and identifications of aquatic turtle species, developed and implemented an environmental DNA assay to detect P. gorzugi, and characterized P. gorzugi habitat. Following an introductory chapter, each task is addressed by chapter and formatted to …